Establishment from an adult leukemic patient of two novel precursor B cell lines with different growth modality

Tarella, Corrado; Pregno, Patrizia; Gallo, Eugenio; Caracciolo, Daniele; Stefani, Stefania; Barletta, C; Lanfrancone, Luisa; Ferrero, Dario

doi:10.1016/0145-2126(90)90047-d

Cited by 5 publications

(1 citation statement)

References 20 publications

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“…PAX5 is also a partner of translocations, and it is the target of point mutations in this disease [28,29]. PC-53, from a 33 year old woman with pre-B ALL at 3rd relapse and the sister cell line PC-53A (at the final, refractory stage) [30], carry the PAX5 R38H mutation (Table 1, Figure 3). This mutation had been described in the context of pre-B ALL (COSM5986423).…”

Section: Mutations and Chromosomal Aberrationsmentioning

confidence: 99%

Molecular Genetics of Pre-B Acute Lymphoblastic Leukemia Sister Cell Lines during Disease Progression

Quentmeier

Pommerenke

Drexler

2021

CIMB

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For many years, immortalized tumor cell lines have been used as reliable tools to understand the function of oncogenes and tumor suppressor genes. Today, we know that tumors can comprise subclones with common and with subclone-specific genetic alterations. We sequenced DNA and RNA of sequential sister cell lines obtained from patients with pre-B acute lymphoblastic leukemia at different phases of the disease. All five pairs of cell lines carry alterations that are typical for this disease: loss of tumor suppressors (CDKN2A, CDKN2B), expression of fusion genes (ETV6-RUNX1, BCR-ABL1, MEF2D-BCL9) or of genes targeted by point mutations (KRAS A146T, NRAS G12C, PAX5 R38H). MEF2D-BCL9 and PAX R38H mutations in cell lines have hitherto been undescribed, suggesting that YCUB-4 (MEF2D-BCL9), PC-53 (PAX R38H) and their sister cell lines will be useful models to elucidate the function of these genes. All aberrations mentioned above occur in both sister cell lines, demonstrating that the sisters derive from a common ancestor. However, we also found mutations that are specific for one sister cell line only, pointing to individual subclones of the primary tumor as originating cells. Our data show that sequential sister cell lines can be used to study the clonal development of tumors and to elucidate the function of common and clone-specific mutations.

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Section: Mutations and Chromosomal Aberrationsmentioning

confidence: 99%