k-Casein upregulates osteogenic differentiation on bone marrow mesenchymal stem cells cultured on agarose microcarriers

Aiyelabegan, Hammed Tanimowo; Ebadi, Malihe; Kardar, Gholam Ali; Lotfibakhshaiesh, Nasrin; Dorkoosh, Farid Abedin; Ebrahimi‐Barough, Somayeh; Sadroddiny, Esmaeil

doi:10.1080/00914037.2019.1570511

Cited by 4 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fibronectin for example, is commonly used to coat plastic or glass microcarriers to increase cell adhesion in microcarrier cultures, and used in concentrations ranging from 1-50 ug/mL [53][54][55]. On the other hand, compounds such as casein, chitosan, or even PNIPAAm was grafted on the surface of microcarriers to modify its adhesion properties and to provide an easier solution for cell harvesting [51,56,57].…”

Section: Microcarrier In Msc Culturementioning

confidence: 99%

“…An increasing number of studies have highlighted bone formation potential by using microcarrier cultures, for example, a new process developed by Zhang et al fabricated pre-vascularized bone microtissues by integrating microcarrier culture and co-culture with MS and HUVEC [65]. Aside from that, Tanimowo et al fabricated a novel agarose-k-casein microsphere which upregulated the expression of osteogenic differentiation markers in bone marrow MSCs [57]. A titanium phosphate glass microcarrier that enhances bone morphogenic protein-2 (BMP-2) and osteopontin (OPN) expression by h-MSC was introduced.…”

Section: Msc Differentiationmentioning

confidence: 99%

See 1 more Smart Citation

Three dimensional microcarrier system in mesenchymal stem cell culture: a systematic review

et al. 2020

View full text Add to dashboard Cite

Stem cell-based regenerative medicine is a promising approach for tissue reconstruction. However, a large number of cells are needed in a typical clinical study, where conventional monolayer cultures might pose a limitation for scaleup. The purpose of this review was to systematically assess the application of microcarriers in Mesenchymal Stem Cell cultures. A comprehensive search was conducted in Medline via Ebscohost, Pubmed, and Scopus, and relevant studies published between 2015 and 2019 were selected. The literature search identified 53 related studies, but only 14 articles met the inclusion criteria. These include 7 utilised commercially available microcarriers, while the rest were formulated based on different surface characteristics, all of which are discussed in this review. Current applications of microcarriers were focused on MSC expansion and induction of MSCs into different lineages. These studies demonstrated that MSCs could proliferate in a microcarrier culture system in-fold compared to monolayer cultures, and the culture system could simulate a three-dimensional environment which induces cell differentiation. However, detailed studies are still required before this system were to be adapted into the scale of GMP manufacturing.

show abstract

Section: Microcarrier In Msc Culturementioning

confidence: 99%

Section: Msc Differentiationmentioning

confidence: 99%

Three dimensional microcarrier system in mesenchymal stem cell culture: a systematic review

et al. 2020

View full text Add to dashboard Cite

show abstract

“…[71] Aiyelabegan et al investigated mesenchymal stem cell (MSC) cultivation on bone-mimetic scaffolds using agarose microgels coated with k-Casein. [164] The microgels upregulated osteogenic differentiation in the MSCs, and the resulting cells exhibited high viability during the 21-day cultivation period. Wang et al developed a dualloaded system for enhanced bone growth comprising fluorescein-labeled chitosan microgels and rhodamine-labeled PLGA microgels connected by an alginate-coated hydroxyapatite scaffold.…”

Section: Bioactive and Bio-adhesive Monomersmentioning

confidence: 97%

Surface‐Bound Microgels for Separation, Sensing, and Biomedical Applications

Cutright

Harris

Ramesh

et al. 2021

Adv Funct Materials

View full text Add to dashboard Cite

This study presents a comprehensive survey of microgel-coated materials and their functional behavior, describing the complex interplay between the physicochemical and mechanical properties of the microgels and the chemical and morphological features of substrates. The cited literature is articulated in four main sections: i) properties of 2D and 3D substrates, ii) synthesis, modification, and characterization of the microgels, iii) deposition techniques and surface patterning, and iv) application of microgel-coated surfaces focusing on separations, sensing, and biomedical applications. Each section discussesby way of principles and examples -how the various design parameters work in concert to deliver functionality to the composite systems. The case studies presented herein are viewed through a multi-scale lens. At the molecular level, the surface chemistry and the monomer make-up of the microgels endow responsiveness to environmental and artificial physical and chemical cues. At the micro-scale, the response effects shifts in size, mechanical, and optical properties, and affinity towards species in the surrounding liquid medium, ranging from small molecules to cells. These phenomena culminate at the macro-scale in measurable, reversible, and reproducible effects, aiming in a myriad of directions, from lab-scale to industrial applications.

show abstract

“…206,208,211 For example, BMSCs could be expanded on agarose or gelatin-based microspheres and the osteogenic and chondrogenic differentiation potential was enhanced compared with a 2D monolayer culture system. 197,212 Moreover, NK cells were expanded to 387 AE 100-fold in 10 days via a dissolvable polymer-based microsphere platform and maintained ideal cell viability and purity. 213 Similarly, HUMSCs were expanded on degradable CS-based microspheres and could increase 4-fold within 5 days' culture.…”

Section: Cell Expansionmentioning

confidence: 99%