Juvenile Capri-Paratuberculosis (JCP) in India: Incidence and characterization by six diagnostic tests

Kumar, Pradeep; Singh, Shoor Vir; Bhatiya, A.K.; Sevilla, Iker A.; Singh, Ajay Vir; Whittington, Richard J.; Juste, Ramón A.; Gupta, Vinod K.; Singh, Pravin Kumar; Sohal, J.S.; Vihan, V. S.

doi:10.1016/j.smallrumres.2006.10.023

Cited by 30 publications

(36 citation statements)

References 22 publications

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“…paratuberculosis (MAP) and is a concern in developed countries 1 . Information on prevalence of JD in India possessing 16.2% (120 million) goat population of the world is limited 2,3,4 . In India, bio-burden of low producing goats and sheep is very high and is taxing the scarce and fragile grazing resources.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of an indigenous ELISA for diagnosis of Johne’s disease and its comparison with commercial kits

Singh¹,

Singh²,

Singh³

et al. 2007

Indian J Microbiol

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Country lacks sensitive and indigenous diagnostic kits for the screening of goats and sheep against Johne's disease. Therefore an indigenous ELISA kit was developed using protoplasmic antigen from native Mycobacterium avium subspecies paratuberculosis 'Bison Type' strain of goat origin (Kit 1). In the present study, kit 1 and two commercial kits (kit 2 and 3) were evaluated with respect to 'Gold Standard' fecal culture in 71 animals (55 goats and 16 sheep). Kit 1 using indigenous antigen (protoplasmic antigen) was sensitive at very low concentration (0.1 μgm / well) as compared to purifi ed commercial protoplasmic antigen (4 μgm / well) used in kit 2, in the Type 1 reactors (strong positive as positive). Screening of 71 animals by fecal culture detected 38.0% animals (goats-40.0%, sheep-31.2%) as positive (clinical shedders of bacilli) from these farm animals. Of the farm animals located at Central Institute for Research on Goats, herds of goat were endemic whereas, sheep fl ocks were comparatively resistant to Johne's disease. The 29.5 and 61.9, 15.4 and 57.7 and 4.2 and 14.0% animals (goats and sheep) were in the category of sero-reactors type 1 and 2 of the ELISA kits 1, 2 and 3, respectively. In the type 1 sero-reactors, sensitivity and specifi city of kit 1, 2 and 3 was 53.7 and 86.0, 17.8 and 86.0 and 3.5 and 94.7%, respectively. Indigenous ELISA test (kit 1) was signifi cantly superior for the screening of goatherds and sheep fl ocks against JD as compared to commercial ELISA kits (Kit 2 and 3).

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Section: Introductionmentioning

confidence: 99%

Evaluation of an indigenous ELISA for diagnosis of Johne’s disease and its comparison with commercial kits

Singh¹,

Singh²,

Singh³

et al. 2007

Indian J Microbiol

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“…However, both the tests (ELISA and AGID) together detected 33.82% (23/68) serum samples as positive ( In the present study, only 14.28% of the youngest animals (<1 year of age) were found sero-positive rest all were above one year of age which means age has a significant role in disease progression. In a similar study, 47.9% of goat kids were found positive by ELISA (Kumar et al, 2007). According to the metaanalysis of Windsor and Whittington (2009) younger animals are susceptible to MAP infection because intestinal mucosa of newborn is highly permeable and thus it allows MAP to penetrate through the mucosal barrier (Sweeney, 1996).…”

Section: Resultsmentioning

confidence: 99%

Sero-Prevalence of Mycobacterium Avium Subspecies Paratuberculosis Infection in Gaddi Goats of Himachal Pradesh

Thakur¹,

Maity²,

Gupta³

2017

Int. J. Livest. Res.

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“…Recent advances in diagnostics, prophylaxis/vaccination alternative therapeutic modalities (Paolicchi et al, 2003;Tripathi et al, 2006;Kumar et al, 2007Kumar et al, , 2014Munjal et al, 2007;Deb et al, 2011; , 2013a,b,c,d, 2014Mahima et al, 2012;Barad et al, 2014) need to be explored fully in the era of emerging antibiotic resistance. Along with these follow up of appropriate/effective preventive and control measures are very crucial for alleviating high financial impacts due to this important pathogen of animals having zoonotic concerns keeping in view One Health, One Medicine approach (Grant, 2005;Tripathi et al, 2002;Momotani, 2012;Sweeney et al, 2012;Dhama et al, 2013e).…”

Section: Vaccinationmentioning

confidence: 99%

Recent Approaches in Diagnosis and Control of Mycobacterial Infections with Special Reference to Mycobacterium Avium Subspecies

Singh¹,

Sohal²,

Kumar³

et al. 2014

Adv. Anim. Vet. Sci.

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Juvenile Capri-Paratuberculosis (JCP) in India: Incidence and characterization by six diagnostic tests

Cited by 30 publications

References 22 publications

Evaluation of an indigenous ELISA for diagnosis of Johne’s disease and its comparison with commercial kits

Evaluation of an indigenous ELISA for diagnosis of Johne’s disease and its comparison with commercial kits

Sero-Prevalence of Mycobacterium Avium Subspecies Paratuberculosis Infection in Gaddi Goats of Himachal Pradesh

Recent Approaches in Diagnosis and Control of Mycobacterial Infections with Special Reference to Mycobacterium Avium Subspecies

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