Just the FACS

Lanier, Lewis L.

doi:10.4049/jimmunol.1401725

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…1). For example, the Coulter counter and the flow cytometer revolutionized high-throughput single-cell analysis 1 , and DNA sequencing in small-bore capillary tubes markedly accelerated the progress of the Human Genome Project 2 .…”

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confidence: 99%

Microfluidics: reframing biological enquiry

Duncombe

Tentori

Herr

2015

Nat Rev Mol Cell Biol

267

202

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The underlying physical properties of microfluidic tools have led to new biological insights through the development of microsystems that can manipulate, mimic and measure biology at a resolution that has not been possible with macroscale tools. Microsystems readily handle sub-microlitre volumes, precisely route predictable laminar fluid flows and match both perturbations and measurements to the length scales and timescales of biological systems. The advent of fabrication techniques that do not require highly specialized engineering facilities is fuelling the broad dissemination of microfluidic systems and their adaptation to specific biological questions. We describe how our understanding of molecular and cell biology is being and will continue to be advanced by precision microfluidic approaches and posit that microfluidic tools — in conjunction with advanced imaging, bioinformatics and molecular biology approaches — will transform biology into a precision science.

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confidence: 99%

Microfluidics: reframing biological enquiry

Duncombe

Tentori

Herr

2015

Nat Rev Mol Cell Biol

267

202

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“…Fluorescent-activated cell sorting is a specialized type of flow cytometry that was developed in the 1960s [66]. It involves suspending and vibrating cells that have been stained with reagents (antibodies and antigens) that were previously tagged with fluorescent molecules.…”

Section: Single B-cell Technologymentioning

confidence: 99%

Bioengineering of Antibody Fragments: Challenges and Opportunities

et al. 2023

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Antibody fragments are used in the clinic as important therapeutic proteins for treatment of indications where better tissue penetration and less immunogenic molecules are needed. Several expression platforms have been employed for the production of these recombinant proteins, from which E. coli and CHO cell-based systems have emerged as the most promising hosts for higher expression. Because antibody fragments such as Fabs and scFvs are smaller than traditional antibody structures and do not require specific patterns of glycosylation decoration for therapeutic efficacy, it is possible to express them in systems with reduced post-translational modification capacity and high expression yield, for example, in plant and insect cell-based systems. In this review, we describe different bioengineering technologies along with their opportunities and difficulties to manufacture antibody fragments with consideration of stability, efficacy and safety for humans. There is still potential for a new production technology with a view of being simple, fast and cost-effective while maintaining the stability and efficacy of biotherapeutic fragments.

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“…FACS was first described in 1969 (Hulett, Bonner, Barrett & Herzenberg, 1969) by Leonard Herzenberg and a team of engineers at Stanford University who developed a machine that could detect fluorescent cells in a liquid stream of droplets (disrupted by a piezocrystal) and sort them by applying an electrical charge (see Figure 3A). Subsequently, with the incorporation of an argon laser, they had achieved a purity of approximately 50% (Julius, Masuda & Herzenberg, 1972; Lanier, 2014). It is now possible to achieve purities of greater than 98% using this method (Harzer, Berger, Conder, Schmauss & Knoblich, 2013).…”

Section: Profiling Transcriptional Activity and Protein-dna Interamentioning

confidence: 99%

Getting Down to Specifics

McClure

Southall

2015

Advances in Genetics

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The majority of multicellular organisms are comprised of an extraordinary range of cell types, with different properties and gene expression profiles. Understanding what makes each cell type unique, and how their individual characteristics are attributed, are key questions for both developmental and neurobiologists alike. The brain is an excellent example of the cellular diversity expressed in the majority of eukaryotes. The mouse brain comprises of approximately 75 million neurons varying in morphology, electrophysiology, and preferences for synaptic partners. A powerful process in beginning to pick apart the mechanisms that specify individual characteristics of the cell, as well as their fate, is to profile gene expression patterns, chromatin states, and transcriptional networks in a cell type-specific manner, i.e. only profiling the cells of interest in a particular tissue. Depending on the organism, the questions being investigated, and the material available, certain cell type-specific profiling methods are more suitable than others. This chapter reviews the approaches presently available for selecting and isolating specific cell types and evaluates their key features.

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Just the FACS

Cited by 6 publications

References 13 publications

Microfluidics: reframing biological enquiry

Microfluidics: reframing biological enquiry

Bioengineering of Antibody Fragments: Challenges and Opportunities

Getting Down to Specifics

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