Jolkinolide B from Euphorbia fischeriana Steud Induces Apoptosis in Human Leukemic U937 Cells through PI3K/Akt and XIAP Pathways

Wang, Jiahe; Zhou, Yijun; Bai, Xue; He, Ping

doi:10.1007/s10059-011-0137-0

Cited by 45 publications

(32 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…JB is a diterpenoid component isolated from the dried roots of Euphorbia fischeriana Steud . It has been reported that JB regulates proliferation and induces apoptosis in human leukemic U937 cell7. Another document evaluates the anti-metastatic role and mechanisms of JB on breast cancer MDA-MB-231 cells by the integrin/FAK and ERK pathways in vitro 8.…”

mentioning

confidence: 99%

Jolkinolide B induces apoptosis and inhibits tumor growth in mouse melanoma B16F10 cells by altering glycolysis

Gao

Yan

Wang

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Most cancer cells preferentially rely on glycolysis to produce the energy (adenosine triphosphate, ATP) for growth and proliferation. Emerging evidence demonstrates that the apoptosis in cancer cells could be closely associated with the inhibition of glycolysis. In this study, we have found that jolkinolide B (JB), a bioactive diterpenoid extracted from the root of Euphorbia fischeriana Steud, induced tumor cells apoptosis and decreased the production of ATP and lactic acid in mouse melanoma B16F10 cells. Furthermore, we found that JB downregulated the mRNA expression of glucose transporter genes (Glut1, Glut3 and Glut4) and glycolysis-related kinase genes (Hk2 and Ldha) in B16F10 cells. Moreover, treatment with JB upregulated the mRNA expression of pro-apoptosis genes (Bax), downregulated the mRNA expression of anti-apoptosis genes (Bcl-2, Caspase-3 and Caspase-9), decreased the potential of mitochondrial membrane and increased reactive oxygen species (ROS) levels in B16F10 cells. Finally, intragastric administration of JB suppressed tumor growth and induced tumor apoptosis in mouse xenograft model of murine melanoma B16F10 cells. Taken together, these results suggest that JB could induce apoptosis through the mitochondrial pathway and inhibit tumor growth. The inhibition of glycolysis could play a crucial role in the induction of apoptosis in JB-treated B16F10 cells.

show abstract

mentioning

confidence: 99%

Jolkinolide B induces apoptosis and inhibits tumor growth in mouse melanoma B16F10 cells by altering glycolysis

Gao

Yan

Wang

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…An increasing number of studies indicate that diterpenoids from E. fischeriana Steud, such as 17-hydroxy-jolkinolide B, 17-acetoxyjolkinolide B and jolkinolide B, are able to induce apoptosis in tumor cells, including human leukemic U937, MDA-MB-231 and HepG2 cells, by modulating the IκB kinase, signal transducer and activator of transcription, or phosphoinositide 3-kinase/Akt pathways (10,20,21). Several studies have also suggested that diterpenoid compounds are able to inhibit cell-ECM adhesion (13,22).…”

Section: Discussionmentioning

confidence: 99%

“…Jolkinolide B is a biologically active chemical isolated from Euphorbia fischeriana Steud. Previous studies have revealed that this chemical acts as an anticancer agent (10,11). However, it is not clear whether jolkinolide B exerts anti-metastasis effects by suppressing the expression of adhesion molecules, such as β 1 -integrin.…”

Section: Introductionmentioning

confidence: 99%

Anti-metastatic effect of jolkinolide B and the mechanism of activity in breast cancer MDA-MB-231 cells

et al. 2015

View full text Add to dashboard Cite

Abstract. Tumor metastasis is the main cause of mortality in cancer patients. However, no effective therapies are currently available to prevent metastasis. Cell adhesion to the extracellular matrix (ECM) is crucial in cancer progression and metastasis. Thus, suppression of cell adhesion may be an effective therapeutic strategy for the prevention of metastasis. In the present study, the anti-adhesion and anti-invasion effects of jolkinolide B, a diterpenoid compound from Euphorbia fischeriana Steud, that were exerted through suppression of β 1 -integrin expression and phosphorylation of focal adhesion kinase (FAK) were examined in human breast cancer MDA-MB-231 cells. Jolkinolide B inhibited the adhesion of MDA-MB-231 cells to fibronectin but not to poly-L-lysine. In addition, jolkinolide B inhibited extracellular signal-regulated kinase (ERK) phosphorylation. U0126, an ERK inhibitor, also suppressed the invasion and adhesion of MDA-MB-231 cells. Overall, the present data demonstrated that jolkinolide B is a novel inhibitor of FAK-mediated signaling pathways that is involved in decreasing cell adhesion and invasion. Mitogen-activated protein kinase/ERK kinase may play a critical role in these effects, indicating that jolkinolide B possesses therapeutic potential for the treatment of breast cancer metastasis.

show abstract

“…The cytotoxicity of all 13 compounds was tested by MTT method (Wang et al 2009(Wang et al , 2011. The result showed that compounds 1 and 2 had an intensive effect with IC 50 levels of 23.…”

Section: Cytotoxicitymentioning

confidence: 99%

Two new ent-atisanes from the root of Euphorbia fischeriana Steud.

Wang

Wei

et al. 2015

Natural Product Research

View full text Add to dashboard Cite

Two new ent-atisanes ent-1β,3β,16β,17-tetrahydroxyatisane (1), ent-1β,3α,16β,17-tetrahydroxyatisane (2) together with 11 known diterpenes were isolated from the anti-tumour activity fraction of Euphorbia fischeriana Steud. The compounds were identified by detailed spectroscopic analysis, including extensive 2D-NMR experiments. X-ray analysis was applied to determine the structure of compound 2. All 13 compounds were screened for cytotoxicity in vitro against human tumour MCF-7, HepG-2 and SGC-7901 cell lines. Compounds 1 and 2 showed the inhibitory effects against MCF-7 with IC50 levels of 23.21 and 15.42 μM; simultaneously, compounds 4, 6, 8 and 11 also had definite inhibitory effect against different cell lines.

show abstract

Jolkinolide B from Euphorbia fischeriana Steud Induces Apoptosis in Human Leukemic U937 Cells through PI3K/Akt and XIAP Pathways

Cited by 45 publications

References 25 publications

Jolkinolide B induces apoptosis and inhibits tumor growth in mouse melanoma B16F10 cells by altering glycolysis

Jolkinolide B induces apoptosis and inhibits tumor growth in mouse melanoma B16F10 cells by altering glycolysis

Anti-metastatic effect of jolkinolide B and the mechanism of activity in breast cancer MDA-MB-231 cells

Two new ent-atisanes from the root of Euphorbia fischeriana Steud.

Contact Info

Product

Resources

About