Jacalin, a jackfruit lectin, precipitates IgA1 but not IgA2 subclass on gel diffusion reaction

Kondoh, Hozumi; Kobayashi, Kunihiko; Hagiwara, Keiji; Kajii, Tadashi

doi:10.1016/0022-1759(86)90003-7

Cited by 78 publications

(3 citation statements)

References 9 publications

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“…[32], Roque-Barreira et al . [33] and Kondoh et al [34], were among the first who identified jacalin around 1980. Jacalin had the property of binding human IgA with specificity for the IgA1 subclass [32,33,34].…”

Section: Flowering Plant Lectinsmentioning

confidence: 99%

“…[33] and Kondoh et al [34], were among the first who identified jacalin around 1980. Jacalin had the property of binding human IgA with specificity for the IgA1 subclass [32,33,34]. In the recent years various studies have been carried out on jacalin, revealing its importance as a lectin of diverse applications ranging from the isolation of human IgA1 to HIV research.…”

Section: Flowering Plant Lectinsmentioning

confidence: 99%

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Lectins with Anti-HIV Activity: A Review

Akkouh

Singh

et al. 2015

Molecules

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Lectins including flowering plant lectins, algal lectins, cyanobacterial lectins, actinomycete lectin, worm lectins, and the nonpeptidic lectin mimics pradimicins and benanomicins, exhibit anti-HIV activity. The anti-HIV plant lectins include Artocarpus heterophyllus (jacalin) lectin, concanavalin A, Galanthus nivalis (snowdrop) agglutinin-related lectins, Musa acuminata (banana) lectin, Myrianthus holstii lectin, Narcissus pseudonarcissus lectin, and Urtica diocia agglutinin. The anti-HIV algal lectins comprise Boodlea coacta lectin, Griffithsin, Oscillatoria agardhii agglutinin. The anti-HIV cyanobacterial lectins are cyanovirin-N, scytovirin, Microcystis viridis lectin, and microvirin. Actinohivin is an anti-HIV actinomycete lectin. The anti-HIV worm lectins include Chaetopterus variopedatus polychaete marine worm lectin, Serpula vermicularis sea worm lectin, and C-type lectin Mermaid from nematode (Laxus oneistus). The anti-HIV nonpeptidic lectin mimics comprise pradimicins and benanomicins. Their anti-HIV mechanisms are discussed.

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Section: Flowering Plant Lectinsmentioning

confidence: 99%

Section: Flowering Plant Lectinsmentioning

confidence: 99%

Lectins with Anti-HIV Activity: A Review

Akkouh

Singh

et al. 2015

Molecules

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“…Serum and milk IgA antibody was purified by jacalin adsorption as previously described with some modifications (17). Jacalin is a lectin that binds specifically to the human IgA immunoglobulin of subclass IgA1 (18). Briefly, 100 KL of jacalin-agarose beads (Sigma) was added to 100 KL of each serum samples.…”

Section: Iga Antibody Purificationmentioning

confidence: 99%

Anti‐rotavirus Antibodies in Human Milk

Asensi¹,

Martínez‐Costa²,

Buesa³

2006

J. pediatr. gastroenterol. nutr.

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Viral proteins VP2, VP6, and NSP2 are strongly precipitated by serum and fecal antibodies from children with rotavirus symptomatic infection

et al. 1998

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Rotavirus-specific IgA has been correlated with immune protection against rotavirus reinfection and symptomatic disease. Systemic and mucosal antibody responses were determined by an enzyme-linked immunosorbent assay in 11 infants with severe rotavirus gastroenteritis. Geometric mean titers of antirotavirus serum IgG and IgA antibodies were significantly higher during the convalescence of the disease (P < 0.001 vs. acute-phase titers). Rotavirus-specific fecal sIgA antibodies increased 4 times during the convalescence in 9 (81.8%) children (P < 0.001). The serum IgG and IgA antibody and fecal sIgA antibody responses to individual rotavirus polypeptides were characterized by radioimmunoprecipitation assay (RIPA) using Staphylococcus aureus protein A and the lectin jacalin to precipitate IgG- and IgA-immune complexes, respectively. The main IgG response was directed toward the structural viral proteins VP2, VP4, and VP6 and toward the nonstructural protein NSP2. Serum IgA reactivity was detected by RIPA in all serum samples, with major responses to VP2, VP6, and NSP2. Interestingly, fecal sIgA in convalescent samples reacted strongly toward NSP2 and VP6. These data reinforce the antigenic importance of rotaviral proteins other than VP4 and VP7, such as VP2, VP6, and NSP2, as main targets in the immune response to rotavirus.

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Jacalin, a jackfruit lectin, precipitates IgA1 but not IgA2 subclass on gel diffusion reaction

Cited by 78 publications

References 9 publications

Lectins with Anti-HIV Activity: A Review

Lectins with Anti-HIV Activity: A Review

Anti‐rotavirus Antibodies in Human Milk

Viral proteins VP2, VP6, and NSP2 are strongly precipitated by serum and fecal antibodies from children with rotavirus symptomatic infection

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