Jabuticaba (Myrciaria cauliflora) Seeds: Chemical Characterization and Extraction of Antioxidant and Antimicrobial Compounds

Abstract

This study was aimed to assess the effect of time and temperature on the extraction of antioxidant compounds from jabuticaba seeds (Myrciaria cauliflora cv. Sabará), to optimize the solvent proportion (water, ethyl alcohol, and propanone), and to characterize the extract according to the chemical composition, antioxidant, and antimicrobial properties. Proximal composition, total phenolic content (TPC), antioxidant, and antimicrobial activities were analyzed. The optimized solvent ratio of 60% water and 40% propanone provided a mean TPC of 8.65 g GAE/100 g seeds and the antioxidant activity toward 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 82.79% ± 0.50%. Time and temperature parameters did not influence the yield of TPC. The gross seed extract was partially purified and both exhibited a high antioxidant activity and antimicrobial potential toward Gram-positive and Gram-negative bacteria. The purified jabuticaba seed lyophilized extract contained a higher (P < 0.05) TPC, o-diphenols, flavonols, and antioxidant activity measured by the DPPH assay and total reducing capacity as compared to the gross lyophilized extract. Electrospray ionization coupled with tandem mass spectrometry (ESI-MS/MS) data showed the presence of ellagitannins and ellagic acid in the extracts, which are probably the responsible for the antimicrobial and antioxidant activities.

Polyphenols from Allanblackia floribunda seeds: Identification, quantification and antioxidant activity

“…These findings are consistent with aglycone activities observed in the current study. Overall, the data presented here demonstrates that Allanblackia seeds are an abundant source of highly active antioxidant phenolic components in common with seeds of the fruits of other tropical species including jabuticaba (Myrciaria cauliflora; Hacke et al, 2016) and guaraná (Paullinia cupana; Majhenič, Škerget & Knez, 2007;Marques et al 2016).…”

Antioxidants Distribution in Pulp and Seeds of Black and Red Goji Berries as Affected by Boiling Processing

“…They presented that the TPC in fresh and dried goji fruits are 174.27 and 342.59 gallic acid equivalents/100 g, respectively, using ethanol 80% as extract solvent. Moreover, the other group measured a mean TPC of 8.65 g GAE/100 g seeds in Jabuticaba (Myrciaria cauliflora) seeds [21].…”

Optimizing the Extraction of Anthocyanins from the Skin and Phenolic Compounds from the Seed of Jabuticaba Fruits (Myrciaria jabuticaba (Vell.) O. Berg) with Ternary Mixture Experimental Designs

“…Hacke et al 18 reported 8.65 g GAE 100 g -1 d.w. in "Paulista" jabuticaba seed, a result that is in agreement with this study for "Paulista" jabuticaba harvested in the 2014 and 2015 crops season (7.20 and 11.91 g GAE 100 g -1 d.w., respectively).…”

“…Moreover, the great majority of the studies are based on the skin, and we only found one study that optimized the TPC extraction of the jabuticaba seed. 18 The available protocols for these purposes, even those that approached the optimization of the extraction method, require very long extraction times and processes not accessible to all laboratories.…”