JA-mediated transcriptional regulation of secondary metabolism in medicinal plants

Afrin, Sadia; Huang, Jingjia; Luo, Zhitian

doi:10.1007/s11434-015-0813-0

Cited by 89 publications

(56 citation statements)

References 92 publications

(52 reference statements)

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“…Since AaWRKY1, AaERF1, AaERF2, AaORA, AabZIP1, and AabHLH1 transcript levels are increased by abiotic stress, such as ABA and JA, it is interesting to expand the network on how abiotic stress stimulates transcriptional levels of biosynthetic genes. The phytohormone jasmonates play an important role in regulating plant secondary metabolism, especially in medicinal plants [11,75], such as vinblastine biosynthesis in C. roseus [76], nicotine biosynthesis in N. tabacum [67], terpenoids biosynthesis in A. annua [77], Taxus chinensis [78], and Panax ginseng [75,79]. Nowadays, more and more researches in A. annua show that JA is one of the most effective phytohormones in promoting artemisinin accumulation [77,[80][81][82].…”

Section: Perspectivesmentioning

confidence: 99%

Transcriptional regulation of artemisinin biosynthesis in Artemisia annua L.

Shen

Yan

et al. 2016

Science Bulletin

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Section: Perspectivesmentioning

confidence: 99%

Transcriptional regulation of artemisinin biosynthesis in Artemisia annua L.

Shen

Yan

et al. 2016

Science Bulletin

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“…Several TF families such as MYC, MYB, WRKY, and AP2/ERF are involved in regulating secondary metabolism in different medicinal plants [28]. Therefore, we asked whether DEGs included TFs.…”

Section: Transcription Factors Differentially Expressed In Response Tmentioning

confidence: 99%

“…The JAZ proteins contain a Jas domain that is required for the interaction of both COI1 and a broad array of TFs. In the presence of JA or its bioactive derivatives, JAZ proteins are degraded and freeing TFs for expression of specific sets of JA-responsive genes, regulate enzymes involved in the biosynthesis of secondary metabolites, such as ginsenoside, artemisinin, and vinblastine [28]. We have previously conducted extensive studies on the cultivation, phytochemistry, and pharmacology of A. bidentata and the relationship between plant structure and accumulation of active components.…”

Section: Introductionmentioning

confidence: 99%

NAA and 6-BA promote accumulation of oleanolic acid by JA regulation in Achyranthes bidentata Bl

et al. 2020

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Application of plant growth regulators has become one of the most important means of improving yield and quality of medicinal plants. To understand the molecular basis of phytohormone-regulated oleanolic acid metabolism, RNA-seq was used to analyze global gene expression in Achyranthes bidentata treated with 2.0 mg/L 1-naphthaleneacetic acid (NAA) and 1.0 mg/L 6-benzyladenine (6-BA). Compared with untreated controls, the expression levels of 20,896 genes were significantly altered with phytohormone treatment. We found that 13071 (62.5%) unigenes were up-regulated, and a lot of differentially expressed genes involved in hormone or terpenoid biosynthesis, or transcription factors were significantly upregulated. These results suggest that oleanolic acid biosynthesis induced by NAA and 6-BA occurs due to the expression of key genes involved in jasmonic acid signal transduction. This study is the first to analyze the production and hormonal regulation of medicinal A. bidentata metabolites at the molecular level. The results herein contribute to a better understanding of the regulation of oleanane-type triterpenoid saponins accumulation and define strategies to improve the yield of these useful metabolites. OPEN ACCESSCitation: Liu Y, Tang L, Wang C, Li J (2020) NAA and 6-BA promote accumulation of oleanolic acid by JA regulation in Achyranthes bidentata Bl. PLoS ONE 15(2): e0229490. https://doi.org/10.triterpenoid biosynthetic pathway can be divided into three steps: synthesis of universal terpenoid precursors, formation of carbon skeletons, and modification of triterpenoid skeletons. Triterpenoid precursors are synthesized mainly through the cytoplasmic mevalonate (MVA) pathway. The plastidic 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway is believed to be a minor pathway for triterpenoid biosynthesis [17]. Triterpenoid skeleton synthesis genes include FPS (farnesyl diphosphate synthase), SS (squalene synthase), SE (squalene epoxidase), and OSCs (oxidosqualene cyclases). The cyclization of 2,3-oxidosqualene catalyzed by OSCs is a key step in the biosynthesis of triterpenoid saponins and sterols. β-amyrin synthase (β-AS) and cycloartenol synthase (CAS) catalyze the conversion of 2,3-oxidosqualene to β-amyrin and cycloartenol, which are precursors for oleanolic acid and phytosterols, respectively. A lot is known about several genes involved in modification of the triterpenoid skeleton downstream of the cyclization step are known. Some cytochrome P450 monooxygenases (CYP450s) and glycosyl transferases (GTs) were shown to catalyze modifications of triterpenoid skeletons, including hydroxylation and glycosidation. An increasing number of studies have indicated that the CYP716 family belongs to the CYP85 clan of CYP450s, and is involved in biosynthesis of various triterpenoids including oleanane backbones [18,19]. For example, CYP716A12 is involved in oxidation of the β-amyrin skeleton, which modifies the oleanane backbone [20]. CYP716A52v2 catalyzes the conversion of βamyrin to oleanolic acid in P. ginseng [21]. Howeve...

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“…To this end, we profiled tissues of N. attenuata, N. obtusifolia, N. cavicola and N. sylvestris after treatment with MeJA. MeJA is known to regulate many secondary metabolic pathways at the transcriptional level, and is often used to summarize downstream effects of insect herbivory (Afrin et al, 2015). This plant hormone is well-known to rapidly up-regulate genes of the phenylpropanoid pathway (Gaquerel et al, 2013), as well as those of the shikimate (a) Binary plot indicating the presence (black cells) and absence (open cells) of all putatively identified diterpene glycosides and derivatives based on various sub-groups defined by chemical modifications.…”

Section: Identification Of Conserved Biosynthetic Steps and Their Regmentioning

confidence: 99%

Using the knowns to discover the unknowns: MS‐based dereplication uncovers structural diversity in 17‐hydroxygeranyllinalool diterpene glycoside production in the Solanaceae

et al. 2016

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SUMMARYExploring the diversity of plant secondary metabolism requires efficient methods to obtain sufficient structural insights to discriminate previously known from unknown metabolites. De novo structure elucidation and confirmation of known metabolites (dereplication) remain a major bottleneck for mass spectrometrybased metabolomic workflows, and few systematic dereplication strategies have been developed for the analysis of entire compound classes across plant families, partly due to the complexity of plant metabolic profiles that complicates cross-species comparisons. 17-hydroxygeranyllinalool diterpene glycosides (HGLDTGs) are abundant defensive secondary metabolites whose malonyl and glycosyl decorations are induced by jasmonate signaling in the ecological model plant Nicotiana attenuata. The multiple labile glycosidic bonds of HGL-DTGs result in extensive in-source fragmentation (IS-CID) during ionization. To reconstruct these IS-CID clusters from profiling data and identify precursor ions, we applied a deconvolution algorithm and created an MS/MS library from positive-ion spectra of purified HGL-DTGs. From this library, 251 nonredundant fragments were annotated, and a workflow to characterize leaf, flower and fruit extracts of 35 solanaceous species was established. These analyses predicted 105 novel HGL-DTGs that were restricted to Nicotiana, Capsicum and Lycium species. Interestingly, malonylation is a highly conserved step in HGL-DTG metabolism, but is differentially affected by jasmonate signaling among Nicotiana species. This MS-based workflow is readily applicable for cross-species re-identification/annotation of other compound classes with sufficient fragmentation knowledge, and therefore has the potential to support hypotheses regarding secondary metabolism diversification.

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JA-mediated transcriptional regulation of secondary metabolism in medicinal plants

Cited by 89 publications

References 92 publications

Transcriptional regulation of artemisinin biosynthesis in Artemisia annua L.

Transcriptional regulation of artemisinin biosynthesis in Artemisia annua L.

NAA and 6-BA promote accumulation of oleanolic acid by JA regulation in Achyranthes bidentata Bl

Using the knowns to discover the unknowns: MS‐based dereplication uncovers structural diversity in 17‐hydroxygeranyllinalool diterpene glycoside production in the Solanaceae

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