NAA and 6-BA promote accumulation of oleanolic acid by JA regulation in Achyranthes bidentata Bl

Liu, Yanqing; Tang, Li; Wang, Can; Li, Jinting

doi:10.1371/journal.pone.0229490

Cited by 2 publications

(1 citation statement)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GKK1032. It was confirmed further by the isolation of the triterpenoid molecule soyasapogenol B (11) from an endophytic fungus in Achyranthes bidentata that plant endophytes might interact with their hosts' metabolic process and generate analogs [43]. A bioactive sterol compound known as ergosterol ( 12) is frequently present in plants [29], edible mushrooms [44] and microorganisms [45].…”

Section: Discussionmentioning

confidence: 91%

Endophytic Microbes from Medicinal Plants in Fenghuang Mountain as a Source of Antibiotics

Yang,

Hong,

Zhou

et al. 2023

Molecules

View full text Add to dashboard Cite

One of the largest concerns with world health today is still antibiotic resistance, which is making it imperative to find efficient alternatives as soon as possible. It has been demonstrated that microbes are reliable sources for the creation of therapeutic antibiotics. This research intends to investigate the endophytic microorganisms from several medicinal plants in Fenghuang Mountain (Jiangsu Province, China) and to discover new antibiotics from their secondary metabolites. A total of 269 endophytic strains were isolated from nine distinct medicinal plants. Taxonomic analysis revealed that there were 20 distinct species among these endophytes, with Streptomyces being the most common genus. Three of the target strains were chosen for scale-up fermentation after preliminary screening of antibacterial activities and the metabolomics investigation using LC-MS. These strains were Penicillium sp. NX-S-6, Streptomyces sp. YHLB-L-2 and Streptomyces sp. ZLBB-S-6. Twenty-three secondary metabolites (1–23), including a new sorbicillin analogue (1), were produced as a result of antibacterial activity-guided isolation. Through spectroscopic analysis using MS and NMR, the structures of yield compounds were clarified. According to antibacterial data, S. aureus or B. subtilis were inhibited to varying degrees by sorrentanone (3), emodic acid (8), GKK1032 B (10), linoleic acid (14), toyocamycin (17) and quinomycin A (21). The most effective antimicrobial agent against S. aureus, B. subtilis, E. coli and A. baumannii was quinomycin A (21). In addition, quinomycin A showed strong antifungal activity against Aspergillus fumigatus, Cryptococcus neoformans, and two clinical isolated strains Aspergillus fumigatus #176 and #339, with MIC as 16, 4, 16 and 16 µg/mL, respectively. This is the first time that bioprospecting of actinobacteria and their secondary metabolites from medicinal plants in Fenghuang Mountain was reported. The finding demonstrates the potential of endophytic microbes in medical plants to produce a variety of natural products. Endophytic microbes will be an important source for new antibiotics.

show abstract

Section: Discussionmentioning

confidence: 91%

Endophytic Microbes from Medicinal Plants in Fenghuang Mountain as a Source of Antibiotics

Yang,

Hong,

Zhou

et al. 2023

Molecules

View full text Add to dashboard Cite

show abstract

Genome-wide identification and integrated analysis of the FAR1/FHY3 gene family and genes expression analysis under methyl jasmonate treatment in Panax ginseng C. A. Mey.

Jiang,

Zeng,

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

Ginseng (Panax ginseng C. A. Mey.) is an important and valuable medicinal plant species used in traditional Chinese medicine, and its metabolite ginsenoside is the primary active ingredient. The FAR1/FHY3 gene family members play critical roles in plant growth and development as well as participate in a variety of physiological processes, including plant development and signaling of hormones. Studies have indicated that methyl jasmonate treatment of ginseng adventitious roots resulted in a significant increase in the content of protopanaxadiol ginsenosides. Therefore, it is highly significant to screen the FAR1/FHY3 gene family members in ginseng and preliminarily investigate their expression patterns in response to methyl jasmonic acid signaling. In this study, we screened and identified the FAR1/FHY3 family genes in the ginseng transcriptome databases. And then, we analyzed their gene structure and phylogeny, chromosomal localization and expression patterns, and promoter cis-acting elements, and made GO functional annotations on the members of this family. After that, we treated the ginseng adventitious roots with 200 mM methyl jasmonate and investigated the trend of the expression of four genes containing the largest number of methyl jasmonate cis-acting elements at different treatment times. All four genes were able to respond to methyl jasmonate, the most significant change was in the PgFAR40 gene. This study provides data support for subsequent studies of this family member in ginseng and provides experimental reference for subsequent validation of the function of this family member under methyl jasmonic acid signaling.

show abstract

NAA and 6-BA promote accumulation of oleanolic acid by JA regulation in Achyranthes bidentata Bl

Cited by 2 publications

References 48 publications

Endophytic Microbes from Medicinal Plants in Fenghuang Mountain as a Source of Antibiotics

Endophytic Microbes from Medicinal Plants in Fenghuang Mountain as a Source of Antibiotics

Genome-wide identification and integrated analysis of the FAR1/FHY3 gene family and genes expression analysis under methyl jasmonate treatment in Panax ginseng C. A. Mey.

Contact Info

Product

Resources

About