Isozyme Polymorphism in the Genus Actinidia and the Origin of the Kiwifruit Genome

Testolin, R.; Ferguson, A. R.

doi:10.2307/2419435

Cited by 41 publications

(37 citation statements)

References 21 publications

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“…x 2 -tests were used to determine goodness-of-fit of the segregation ratios among the 40 sexual hybrids. The inheritance of nuclear SSR markers is of polysomic type (see § 3), a finding that is in agreement with the conclusion of Testolin & Ferguson (1997) that A. deliciosa is an autohexaploid. As a consequence, for markers that are only present in the male parent, the probability of allele presence [1] in the male gamete corresponds to random sampling without replacement of three alleles among the six paternal ones.…”

Section: (D ) Statistical Analysis Of the Ssr Datasupporting

confidence: 84%

A one–step organelle capture: gynogenetic kiwifruits with paternal chloroplasts

Chat

Decroocq

Petit³

2003

Proc. R. Soc. Lond. B

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Androgenesis, the development of a haploid embryo from a male nucleus, has been shown to result in the instantaneous uncoupling of the transmission of the organelle and nuclear genomes (with the nuclear genome originating from the male parent only and the organelle genomes from the female parent). We report, for the first time, uncoupling resulting from gynogenesis, in Actinidia deliciosa (kiwifruit), a plant species known for its paternal mode of chloroplast inheritance. After pollen irradiation, transmission of nuclear genes from the pollen parent to the progeny was inhibited, but transmission of the chloroplast genome was not. This demonstrates that plastids can be discharged from the pollen tube into the egg with little or no concomitant transmission of paternal nuclear genes. Such events of opposite inheritance of the organelle and nuclear genomes must be very rare in nature and are unlikely to endanger the longterm stability of the association between the different genomes of the cell. However, they could lead to incongruences between organelle gene trees and species trees and may constitute an alternative to the hybridization/introgression scenario commonly invoked to account for such incongruences.

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Section: (D ) Statistical Analysis Of the Ssr Datasupporting

confidence: 84%

A one–step organelle capture: gynogenetic kiwifruits with paternal chloroplasts

Chat

Decroocq

Petit³

2003

Proc. R. Soc. Lond. B

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“…deliciosa together in one section and A. kolomikta in another (Liang, 1984), as well as with recent phylogenetic studies which propose that A . chinensis, was one of the progenitor species (Crowhurst et al ., 1990 ;Crowhurst & Gardner, 1991) or the sole one (Cipriani & Morgante, 1993 ;Cipriani, 1994;Testolin & Ferguson, 1996) …”

Section: Species-specific Markersmentioning

confidence: 99%

Screening RAPD primers for molecular taxonomy and cultivar fingerprinting in the genus Actinidia

1996

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Eighty ten-base long arbitrary primers were tested for PCR-based DNA amplification of three species of the genus Actinidia (A . deliciosa the kiwifruit, A . chinensis, and A . kolomikta), with the aim of screening species-specific and genotype-specific markers .Of the 80 primers tested, 30 gave an average of 3 .5 bands which were monomorphic within one or two species and absent in the remaining one(s), thus resulting in useful markers for taxonomic and phylogenetic purposes . None of the primers tested produced bands linked to sex . Twenty primers out of the twenty-five selected from a preliminary screening showed high levels of polymorphism, producing two to eleven patterns each from the 13 kiwifruit cultivars examined .We found the Stoffel fragment and the Taq polymerise were both suitable for RAPD analysis, the most noticeable difference being the smaller size of fragments (0.4-1 .2 kb) produced by the former in comparison to the latter (1 .0-3 .4 kb) . We tested also three different annealing temperatures (35, 37, and 39°C) and found the intermediate one best for number of amplified bands and reproducibility of results .

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“…In contrast, Jung et al (2003) placed A. rufa close to A. kolomikta and A. melanandra in the section Leiocarpae because of similarities in the noncoding chloroplast DNA sequences. Recent research based on A. kolomikta, leaf flavonoid composition (Webby et al, 1994) and isozyme patterns (Testolin and Ferguson, 1997) indicated that it was quite distinct from any other species in the section Leiocarpae, although RAPD analysis supports retaining A. kolomikta in Leiocarpae . The results of the crossing experiments undertaken in this study suggest that there is a potentially close relationship between A. kolomikta and A. rufa, although it has been shown that many Actinidia species can be crossed under experimental conditions (Hirsch et al, 2001).…”

Section: Discussionmentioning

confidence: 96%

“…Recent reports describing flavonoid composition (Webby et al, 1994), isozyme patterns (Testolin and Ferguson, 1997), chloroplast and ribosomal DNA sequences (Chat et al, 2004;Cipriani et al, 1998;Li et al, 2002), and Random Amplified Polymorphic DNA (RAPD) Kokudo et al, 2003) analyses support the exclusion of A. rufa from the section Leiocarpae. Analyses of the isozyme patterns (Testolin and Ferguson, 1997) and SSR (simple sequence repeat) molecular markers (Korkovelos et al, 2008) suggest that there is a close relationship between A. rufa and A. hemsleyana in the section Strigosae. In addition, chloroplast and ribosomal DNA sequences (Chat et al, 2004;Li et al, 2002), and RAPD analyses ) also suggest a close relationship between A. rufa and A. hemsleyana in the section Strigosae, and between A. persicina and A. zhejiangensis in the section Stellatae.…”

Section: Discussionmentioning

confidence: 96%