Screening RAPD primers for molecular taxonomy and cultivar fingerprinting in the genus Actinidia

Cipriáni, G.; Bella, R. Di; Testolin, R.

doi:10.1007/bf00023855

Cited by 34 publications

(28 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It produces a large number of easily generated markers without the need of previous knowledge of the genome. The success of RAPD markers for taxonomic and evolutionary studies has been observed in several plant species such as Stylosanthes (Kazan et al, 1993), Malus (Dunemann et al, 1994), Actinidia (Cipriani et al, 1996), Pisum (Hoey et al, 1996), Vigna (Santala et al, 1998) and Ananas (Ruas et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal organization and phylogenetic relationships in Hypochaeris species (Asteraceae) from Brazil

et al. 2005

View full text Add to dashboard Cite

The association of cytogenetic and molecular techniques has contributed to the analysis of chromosome organization and phylogeny in plants. The fluorochrome GC-specific CMA 3 , fluorescent in situ hybridization (FISH) and RAPD (Random Amplified Polymorphic DNA) markers were used to investigate chromosome structure and genetic relationships in Hypochaeris (Asteraceae). Seven species native to South America, and two species introduced from Europe (H. glabra and Hypochaeris sp) were studied. FISH with rDNA probes identified one or two loci of 18S-5.8S-25S rDNA in the South American Hypochaeris species and one locus in the European species. Only one 5S rDNA locus was seen in all species studied. Blocks of GC-rich heterochromatin (CMA-positive bands) associated to 18S-5.8S-25SrDNA loci were detected in all species investigated. Co-location of 5S rDNA and CMA bands was also observed, except for three South American species and Hypochaeris sp. In two South American species, additional CMA bands not related to rDNA were observed on the long arm of chromosome 2, near to the centromere. Hypochaeris glabra exhibited additional CMA-positive signals distributed at pericentromeric regions, on the short arms of all chromosomes. A total of 122 RAPD markers were used to determine the genetic relationships among species. The level of polymorphism was very high, revealing two genetic groups comprising the South American and the European species, thus supporting a previous hypothesis of monophyly of the South American Hypochaeris species. The coefficients of genetic similarity between European and South American species were 0.35, on average. Polymorphism was also high within the two groups. The genetic associations observed with RAPD markers were consistent with chromosome characteristics. Species carrying similar distribution of 45S rDNA loci and CMA-positive signals were included in the same group revealed by RAPDs. Cytogenetic and molecular data support the view that not only chromosome rearrangements, but also changes in DNA sequence took place during the diversification of the South American Hypochaeris species.

show abstract

Section: Introductionmentioning

confidence: 99%

Chromosomal organization and phylogenetic relationships in Hypochaeris species (Asteraceae) from Brazil

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Of these markers, RAPD markers (Wiliams et al, 1990) that result from the PCR amplification of genomic DNA fragments using short oligonucleotides (usually 10-mers) of arbitrary sequence as primers have been widely used for diversity analyses as they are simple to use, cost effective and amplify multiple DNA loci through PCR (Wiliams et al, 1990;Abkenar and Isshiki, 2003;Baig et al, 2009). Other advantages of RAPD include requirement of very small amounts of genomic DNA, elimination of blotting and radio-active detection steps (Cipriani et al, 1996). For these reasons many fruit tree crops have been successfully fingerprinted using RAPD markers, e.g.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Assessment of Acid Lime (Citrus Aurantifolia Swingle) Landraces of Eastern Nepal Using RAPD Markers

Munankarmi

Shrestha²,

Rana

et al. 2014

Int J Appl Sci Biotechnol

View full text Add to dashboard Cite

Acid lime (Citrus aurantifolia Swingle) is an important commercial fruit crop, cultivated from terai to high hill landscapes of Nepal. However, production and productivity is very low due to various reasons including infestations by various diseases and pests, lack of diseases and pests resistant and high yielding varieties. In this context, determination of genetic variation at molecular level is fundamental to citrus breeders for the development of elite cultivars with desirable traits. In the present study, Random Amplified Polymorphic DNA (RAPD) marker technique has been employed to assess genetic diversity in 60 acid lime landraces representing different agro-ecological zones of eastern Nepal. Nine selected arbitrary primers generated 79 RAPD fragments of which 75 were polymorphic (94.94%). Phenogram was constructed by NTSYS-PC ver. 2.21i using UPGMA cluster analysis based on Jaccard's similarity coefficient to deduce overall genetic diversity and relationships of the acidlime genotypes under study. Sixty acid lime landraces formed seven clusters and similarity value ranged from 38% to 98% with an average of 72%. Genetic variation at different agro-ecological zones was assessed using Popgene ver. 1.32 and found 47% to 69.6% polymorphism. Shannon's index and Nei's gene diversity showed highest level of acid lime diversity in Terai zone (PPB, 69.62%; H, 0.213; I, 0.325) followed by mid-hill zone (PPB, 67.09%; H, 0.208; I, 0.317). The results obtained will be useful to citrus breeders for elite cultivar development. The RAPD-PCR technique is found to be the rapid and effective tool for genetic diversity assessment in acid lime landraces of Nepal.

show abstract

“…RAPD assay has the advantage of being easy to use, requiring very small amount of genomic DNA without the need for blotting and radioactive detection (Cipriani et al, 1996;Atienzar et al, 2000), and are moderately reproducible. In this research, an attempt has been made to fingerprint and study their genetic relationship using RAPD markers, which could be very helpful for germplasm management, crop improvement and plant varietals rights.…”

Section: Introductionmentioning

confidence: 99%

Analysis of genetic variation in different banana (Musa species) variety using random amplified polymorphic DNAs (RAPDs)

Jain¹,

Saini²,

Pathak³

et al. 2007

Afr. J. Biotechnol.

View full text Add to dashboard Cite

The banana (Musa acuminata Colla) is considered as an important crop plant due to its high economic value as good dietary source. Here, we analyze the genetic relationship of four different banana varieties that are cultivated in south India. Random amplified polymorphic DNAs (RAPDs) fingerprinting of these banana varieties (Grand Naine, Red Banana, Nendran and Rasthali) carried out by three primers (OPA-19, OPB-18, OPD-16) led to DNA amplification. 43.47% of the amplification products were monomorphic (common to all the genotypes), whereas 30.43% were unique, but only 26.08% revealed the relationship between these genotypes.

show abstract

Screening RAPD primers for molecular taxonomy and cultivar fingerprinting in the genus Actinidia

Cited by 34 publications

References 16 publications

Chromosomal organization and phylogenetic relationships in Hypochaeris species (Asteraceae) from Brazil

Chromosomal organization and phylogenetic relationships in Hypochaeris species (Asteraceae) from Brazil

Genetic Diversity Assessment of Acid Lime (Citrus Aurantifolia Swingle) Landraces of Eastern Nepal Using RAPD Markers

Analysis of genetic variation in different banana (Musa species) variety using random amplified polymorphic DNAs (RAPDs)

Contact Info

Product

Resources

About