Isologous IgG-induced tolerance to benzyl penicilloyl

Hapten-specific T-Cell Unresponsiveness Induced by Benzylpenicilloyl Autologous Gamma Globulin Conjugates in Human Lymphocytes In Vitro

“…To resolve these issues, experiments similar to those reported in this paper must be performed using purified subpopulations of T and B cells. However, because in vitro B-cell response is T-dependent, it may be safe to predict that when the techniques are developed to measure human BPO-specific antibody response in vitro, this response will be suppressed by BPO-HGG conjugates in humans as has already been shown in experimental animals (4).…”

“…Onie area of potential therapeutic application of imimiuntillologic tolerance is allergy. For examnple, it has been showni that immuiitnoglobulin (Ig)E antilbody to the major determinanit of penicillin can be suippressedl in primed animals by the adminiistrationi of benzylpenicilloyl conjugated to either isologouis IgG or to a synthetic carrier such as D-glutamic acidl aind D-1)ysinie (4)(5)(6)(7). Before applyinig the concept of carrier-iniduced tolerance to problems in clinical allergy, it is critical to dcemonstrate that unresponsiveness canl be induced in human lymphocytes in vitro.…”

Improvement of specific immunotherapy by human IgG and modified allergens

“…Thus, IgE synthesis is inhibited or abrogated when the tolerogen is given to the animal either prior or subsequent to the hapten in a form otherwise known to lead to formation of anti-hapten IgE antibodies. This has been shown with 2,4-dinitrophenyl-group (DNP) or benzylpenicilloyl-group (BPO) coupled onto homologous IgG (6,43); with BPO linked to copolymers of D-glutamic acid and D-lysine (D-GL) (11) and with trimellityl coupled onto polyvinylalcohol (PVA) (123,124), PEC-linked allergens. These results led to investigations in which experimental antigens and allergens were coupled to polyethylene glycol (PEG).…”

Nucleic acid-specific suppressor T cells.

“…The nucleosides adenosine (A), guanosine (G), cytosine (C), and thymine ribonucleoside (T) were purchased from Sigma. Keyhole limpet haemocyanin (KLH) (Pacific Biomarine, Venice, CA) was prepared as described (12). Murine IgG2.…”

“…Murine IgG2. was obtained through starch block electrophoresis from sera of BALB/c mice bearing the RPC5 tumor, as described (12). Aminoethylcarboxymethyl-Ficoll (AECM-Ficoll) was prepared according to Inman (13).…”