Isolation, structure, and potential biotechnological applications of the exopolysaccharide from Paenibacillus polymyxa 92

Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020

“…47.91 g L −1 respectively). A similar trend in EPS production was described by Liu et al [38] for the strain P. polymyxa EJS-3 and by Grinev et al [33] for the strain P. polymyxa 92. However, P. polymyxa 2020 produced larger amounts of EPS at the same substrate concentration in the culture medium, as compared with strain P. polymyxa EJS-3 and P. polymyxa 92.…”

“…With 15% sucrose, strain P. polymyxa 2020 produced the largest amounts of EPS (53.78 g L −1 ), whereas P. polymyxa EJS-3 strain produced much less EPS (22.82 g L −1 ) with 16% sucrose [38]. With 10% sucrose, strain P. polymyxa 2020 produced 42.02 g L −1 of EPS, whereas strain P. polymyxa 92 produced less EPS (38.4 g L −1 ) [33]. P. polymyxa 92 was grown in 0.5 L Erlenmeyer flasks, containing 150 mL of the medium with initial pH value of 7.2, at 30˚C for 3-7 days in an orbital shaker-incubator (180 rpm).…”

“…Interest in the biosynthesis and production of different EPSs has increased considerably in recent years, because they are potential candidates and precursors for many commercial applications in different industries, including but not limited to food, petroleum, pharmaceuticals and biomedicine [1]. Some of the most used EPSs are xanthan from Xanthomonas campestris, dextran produced by Leuconostoc mesenteroides, Streptococcus sp., Lactobacillus sp., alginate from Azotobacter sp., Pseudomonas sp., gellan derived from Pseudomonas elodea and Sphingomonas paucimobilis, curdlan from Alcaligenes faecalis, hyaluronan from Streptococcus equi, pullulan from Aureobasidium pullulans, bacterial cellulose from Komagataeibacter sp., and [32,33] and curdlan [34]. Paenibacillus polymyxa is a typical member of the Paenibacillus genus, that include about 200 different species of gram-positive spore-forming bacteria [31,35].…”

“…The enzyme breaks down sucrose into glucose and fructose, with the remainder of the latter transferred to the sucrose acceptor molecule [48]. It has been reported that levansucrase, with strong sucrose hydrolysing activity, is involved in many P. polymyxa strains and may be responsible for the high yield of EPS with sucrose as a carbon source [33,38]. Thus, the strain P. polymyxa 92, isolated from the roots of wheat forms 38.4 g/L of levan in 10% sucrose media [33].…”

“…It has been reported that levansucrase, with strong sucrose hydrolysing activity, is involved in many P. polymyxa strains and may be responsible for the high yield of EPS with sucrose as a carbon source [33,38]. Thus, the strain P. polymyxa 92, isolated from the roots of wheat forms 38.4 g/L of levan in 10% sucrose media [33]. Sugar-containing waste from food production, such as molasses, was used to reduce the cost of levan production [49].…”