Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020

Abstract

Paenibacillus spp. exopolysaccharides (EPSs) have become a growing interest recently as a source of biomaterials. In this study, we characterized Paenibacillus polymyxa 2020 strain, which produces a large quantity of EPS (up to 68 g/L),and was isolated from wasp honeycombs. Here we report its complete genome sequence and full methylome analysis detected by Pacific Biosciences SMRT sequencing. Moreover, bioinformatic analysis identified a putative levan synthetic operon. SacC and sacB genes have been cloned and their products identified as glycoside hydrolase and levansucrase respectively. The Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra demonstrated that the EPS is a linear β-(2→6)-linked fructan (levan). The structure and properties of levan polymer produced from sucrose and molasses were analyzed by FT-IR, NMR, scanning electron microscopy (SEM), high performance size exclusion chromatography (HPSEC), thermogravimetric analysis (TGA), cytotoxicity tests and showed low toxicity and high biocompatibility. Thus, P. polymyxa 2020 could be an exceptional cost-effective source for the industrial production of levan-type EPSs and to obtain functional biomaterials based on it for a broad range of applications, including bioengineering.

Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties

“…Common to all polysaccharides, two bands are observed around the 3280–2930 cm −1 region ( Figure 4 , green): the strong broadband appearing at 3282 cm −1 represents the O-H stretching of hydroxyls’ vibrations, and the weak signal at 2923 cm −1 is due to the C-H stretching peak of CH 2 groups [ 13 , 41 ]. In addition, the IR absorption bands around 970-1145cm −1 ( Figure 4 , blue) are mainly due to C-C and C-O stretching in the pyranoid ring and C-O-C stretching of glycosidic bonds [ 42 , 43 , 44 ]. The bands observed at 1725 cm −1 ( Figure 4 , yellow) and at 1248 cm −1 may be attributed to the acyl substituents present in FucoPol’s structure, namely, the C=O stretching vibrations of carbonyls and the C-O-C vibrations, respectively [ 23 , 43 ].…”

“…The peak around 1603 cm −1 and the peaks in the region between 1400 and 1370 cm −1 ( Figure 4 , yellow and orange) may be assigned to the asymmetric and symmetric stretching of carboxylates from glucuronic acid [ 13 , 23 , 29 ]. The presence of bound water might be indicated by the bending vibration of O-H associated with a peak around 1603 cm −1 ( Figure 4 , yellow) [ 42 , 43 , 44 ].…”

Plant Growth-Promoting Activity of Bacillus sp. PG-8 Isolated From Fermented Panchagavya and Its Effect on the Growth of Arachis hypogea

“…Paenibacillus polymyxa isolated from wasp honeycombs was able to produce 2.4% (w/v) EPS with 5.0% (w/v) sucrose after 48 h (Liyaskina et al, 2021). The source and concentration of sugar in the medium affect EPS production.…”

Exopolysaccharides Producing Bacteria: A Review

“…Mamphogoro et al (2022) reported the whole-genome sequence of P. polymyxa SRT9.1, a plant growth-promoting bacterium consisting of 6,754,470 bp and 7878 coding sequences, with an average G + C content of 45% [250]. Revin and Liyaskina et al obtained a highly productive strain Paenibacillus polymyxa 2020, first isolated from wasp honeycombs capable of producing a greater amount of levan compared to the known P. polymyxa strains (Figure 3) [66]. The complete genome sequencing and methylome analysis of P. polymyxa 2020 were performed.…”

“…The GC content of Paenibacillus DNA ranges from 39 to 59% [255]. Complete genome sequencing and assembly has been done for strains such as P. polymyxa E681 [257], P. polymyxa SC2 [258], P. polymyxa ATCC 842T [259], P. polymyxa 2020 (isolated from wasp honeycombs) [66], P. polymyxa KF-1, soil isolated producer of antibiotics [260], and Paenibacillus sp. Y412MC10 [247], isolated from Obsidian Hot Spring in Yellowstone National Park, Montana, USA.…”