Biosynthesis and prebiotic activity of a linear levan from a new Paenibacillus isolate

Cheng, Rui; Cheng, Long; Zhao, Yang; Wang, Lei; Wang, Shiming; Zhang, Jianfa

doi:10.1007/s00253-020-11088-8

Cited by 12 publications

(6 citation statements)

References 85 publications

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“…Recently, it has been reported that Paenibacillus sp. strain FP01 produced levan with high yield (89.5 g/l) and was outstanding for its exceptionally high conversion rate of sucrose to levan (49.7%) [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Bioactive Levan-Type Exopolysaccharide Produced by Pantoea agglomerans ZMR7: Characterization and Optimization for Enhanced Production

Al-Qaysi

Al-Haideri

AL-Shimmary

et al. 2021

J. Microbiol. Biotechnol.

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Levan is an industrially important, functional biopolymer with considerable applications in the food and pharmaceutical fields owing to its safety and biocompatibility. Here, levan-type exopolysaccharide produced by Pantoea agglomerans ZMR7 was purified by cold ethanol precipitation and characterized using TLC, FTIR, 1 H, and 13 C NMR spectroscopy. The maximum production of levan (28.4 g/l) was achieved when sucrose and ammonium chloride were used as carbon and nitrogen sources, respectively, at 35°C and an initial pH of 8.0. Some biomedical applications of levan like antitumor, antiparasitic, and antioxidant activities were investigated in vitro. The results revealed the ability of levan at different concentrations to decrease the viability of rhabdomyosarcoma and breast cancer cells compared with untreated cancer cells. Levan appeared also to have high antiparasitic activity against the promastigote of Leishmania tropica. Furthermore, levan had strong DPPH radical scavenging (antioxidant) activity. These findings suggest that levan produced by P. agglomerans ZMR7 can serve as a natural biopolymer candidate for the pharmaceutical and medical fields.

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Section: Discussionmentioning

confidence: 99%

Bioactive Levan-Type Exopolysaccharide Produced by Pantoea agglomerans ZMR7: Characterization and Optimization for Enhanced Production

Al-Qaysi

Al-Haideri

AL-Shimmary

et al. 2021

J. Microbiol. Biotechnol.

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“…On the other hand, G and K levan did not affect B. lichniformis (88%), whereas M had a relatively comparable effect to the control. The superiority of M in comparison to G and K could be attributed to the strong and wide hydroxyl group of M. Cheng et al 59 reported a relative abundance of several beneficial bacteria, such as Eubacterium, and a significant increase in Anaerostipes, as well as a decrease in harmful bacteria like Rumini Clostridium , while no detectable change was recorded for Lactobacillus as a result of feed supplementation with levan produced by Paenibacillus sp. strain FP01.…”

Section: Resultsmentioning

confidence: 99%

Coculture of bacterial levans and evaluation of its anti-cancer activity against hepatocellular carcinoma cell lines

Wahab,

Shafey,

Mahrous

et al. 2024

Sci Rep

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This research represents a novel study to assess how coculture affects levan yield, structure, bioactivities, and molecular weight. Among the 16 honey isolates, four bacterial strains recorded the highest levan yield. The Plackett–Burman design showed that the coculture (M) of isolates G2 and K2 had the maximum levan yield (52 g/L) and the effective factors were sucrose, incubation time, and sugarcane bagasse. The CCD showed that the most proper concentrations for maximum levan yield (81 g/L): were 130 g/L of sucrose and 6 g/f of sugarcane bagasse. Levan’s backbone was characterized, and the molecular weight was determined. G2 and K2 isolates were identified based on 16 sRNA as Bacillus megaterium strain YM1C10 and Rhizobium sp. G6-1. M levan had promising antioxidant activity (99.66%), slowed the migration activity to a great extent, and recorded 70.70% inhibition against the hepatoblastoma cell line (HepG2) at 1000 µg/mL. Gene expression analysis in liver cancer cell lines (HePG2) revealed that M levan decreased the expression of CCL20), 2GRB2, and CCR6) genes and was superior to Doxo. While increasing the expression of the IL4R and IL-10 genes. The DNA damage values were significantly increased (P < 0.01) in treated liver cancer cell lines with levan M and Doxo. The results referred to the importance of each of the hydroxyl and carboxyl groups and the molecular weight in levans bioactivities.

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“…Studies have shown that gut bacteria ferment non-digestible carbohydrates to produce SCFA, conferring several health benefits ( Gibson et al., 2017 ; Ojo et al., 2021 ). Clostridiales vadin BB60_group are potential SCFA-producing bacteria ( Cheng et al., 2021 ). SCFA affect gut epithelial integrity, which may regulate exposure of the mucosal immune system to bacteria or innate signals that affect immune tolerance ( Macia et al., 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Integrated multi-omics reveals the beneficial role of chlorogenic acid in improving the growth performance and immune function of immunologically stressed broilers

Liu

Zhang

et al. 2023

Animal Nutrition

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Biosynthesis and prebiotic activity of a linear levan from a new Paenibacillus isolate

Cited by 12 publications

References 85 publications

Bioactive Levan-Type Exopolysaccharide Produced by Pantoea agglomerans ZMR7: Characterization and Optimization for Enhanced Production

Bioactive Levan-Type Exopolysaccharide Produced by Pantoea agglomerans ZMR7: Characterization and Optimization for Enhanced Production

Coculture of bacterial levans and evaluation of its anti-cancer activity against hepatocellular carcinoma cell lines

Integrated multi-omics reveals the beneficial role of chlorogenic acid in improving the growth performance and immune function of immunologically stressed broilers

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