Isolation, Purification and Structural Characterestics of Chondroitin Sulfate from Smooth hound Cartilage: In vitro Anticoagulant and Antiproliferative Properties

Krichen, Fatma; Bougatef, Hajer; Sayari, Nadhem; Capitani, Federica; Amor, Ikram Ben; Koubaa, Imed; Maccari, Francesca; Mantovani, Veronica; Galeotti, Fabio; Volpi, Nicola; Bougatef, Ali

doi:10.1016/j.carbpol.2018.06.040

Cited by 46 publications

(19 citation statements)

References 47 publications

(65 reference statements)

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“…Overall, 85% ethanol-extracted method can be used to obtain ethanol-soluble component without chondroitin sulfate. It has been reported that chondroitin sulfate extracted from the cartilage of smooth-hound, corb (Sciaena umbra) and sturgeon (Acipenser sinensis) had the characteristic absorption peaks of polysaccharides at 200, 210-215, and 206 nm in UV spectrum, respectively (46,48,49). However, ESCH did not show an absorption peak at 200 nm (Figure 1A), which confirmed the electrophoresis result (Figure 1B).…”

Section: Discussionsupporting

confidence: 77%

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Anti-inflammatory and Antioxidant Activity of Peptides From Ethanol-Soluble Hydrolysates of Sturgeon (Acipenser schrenckii) Cartilage

Chu

et al. 2021

Front. Nutr.

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Research has shown that cartilage containing chondroitin sulfate and protein presents versatile bioactivities. Chondroitin sulfate in cartilage is beneficial to activate the immune system while the protein/peptide has not been fully understood. The current study investigated the antioxidant and anti-inflammatory properties of ethanol-soluble hydrolysates of sturgeon cartilage (ESCH) prepared through hot-pressure, enzymatic hydrolysis and ethanol extraction. UV spectrum, IR and agarose gel electrophoresis results suggested the successful exclusion of chondroitin sulfate from peptides. Nitric oxide (NO) floods in cells activated by inflammation. It was inhibited when administrated with ESCH. To further explain the observed anti-inflammatory activity, ESCH was separated with Sephadex G-15 into 3 components, among which F3 showed a higher NO inhibition rate and significantly reduced the production of the proinflammatory cytokine IL-6. In addition, the yield of IL-10 increased. Western blotting suggested that F3 downregulated the NO content and IL-6 level by suppressing Mitogen-activated protein kinases (MAPK) channels. Moreover, both ESCH and F3 showed DPPH and ABTS free radical scavenging abilities which was possibly related to the anti-inflammatory property. These results indicated that ESCH behaved anti-inflammatory and antioxidant activities. Cartilage may be a good source to produce anti-inflammatory peptides.

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Section: Discussionsupporting

confidence: 77%

“…It implied that there may be no free acid group. Values of 1,240-925 cm −1 are the characteristic absorption of D-glucopyranose ring and sulfate groups (31,40,46,48), which descripts the exitance of carbohydrates in ESCH. The characteristic stretching vibration peak of the axial coordination of C-O-S in ESCH appears at 867.34 cm −1 .…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory and Antioxidant Activity of Peptides From Ethanol-Soluble Hydrolysates of Sturgeon (Acipenser schrenckii) Cartilage

Chu

et al. 2021

Front. Nutr.

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“…2). A CS from the smooth hound shark (mustelus) composed mainly of monosulfated disaccharides in position 6 and 4 of the N-acetyl β-D-galactosamine (Krichen et. al., 2018), showed six times lower anticoagulant activity compared to sCS.…”

Section: Effect On Coagulation Time and Mediatorsmentioning

confidence: 99%

“…The variety in the position and degree of sulfation, size, number and disaccharide sequences that are inserted in the CSPGs, make CS a heterogeneous molecules. The many biological effects of CS so far reported include inflammation, cell proliferation, differentiation, migration, tissue morphogenesis and wound repair (Krichen et. al., 2018;Mou et.…”

Section: Introductionmentioning

confidence: 99%

A further unique chondroitin sulfate from the shrimp Litopenaeus vannamei with antithrombin activity that modulates acute inflammation

Palhares

Brito

Lima

et al. 2019

Carbohydrate Polymers

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The detailed structure of a further Chondroitin Sulfate from Litopenaeus vannamei shrimp (sCS) is described. The backbone structure was established by 1 H/ 13 C NMR, which identified 3-O-sulfated GlcA, 4-O-sulfated GalNAc, 6-O-sulfated GalNAc, and 4,6-di-O-sulfated GalNAc residues. GlcA is linked to GalNAc 4,6 di S and GlcA 3S is linked to GalNAc 4S, GalNAc 4,6 di-S and GalNAc6S residues. The anticoagulant properties of this sCS were evaluated by activated partial thromboplastin time, anti-IIa, anti-Xa and anti-heparin cofactor II-mediated activities, and sCS failed to stabilise antithrombin in a fluoresence shift assay. The anti-inflammatory effect of sCS was explored using a model of acute peritonitis, followed by leukocyte count and measurement of the cytokines, IL-1β, IL-6 and TNF-α. The compound showed low clotting effects, but high anti-IIa activity and HCII-mediated thrombin inhibition. Its anti-inflammatory effect was shown by leukocyte recruitment inhibition and a decrease in pro-inflammatory cytokine levels. Although the biological role of sCS remains unknown, its properties indicate that it is suitable for studies of multi-potent molecules obtained from natural sources.

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“…The separation of ChS from cartilage usually includes the steps of hydr cartilage, breakdown of the proteoglycan core, protein elimination, and ChS recov hydrolysis of cartilage is usually performed using alkali or enzyme methods. Alc been used to hydrolyze the smooth hound cartilage at 50 °C for 24 h to obtain 2 [20]. Although commercial papain has been used to degrade the proteoglyca cartilage of buffalo [21], lesser spotted dogfish [22], crocodile, and ray [23 temperature of 50-65 °C, enzymatic-assisted extraction requires a long time.…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic-Assisted Extraction and Structural Characterization of Chondroitin Sulfate Derived from Jumbo Squid Cartilage

Yang

Tsai

Shieh

et al. 2021

Foods

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Chondroitin sulfate (ChS) is usually used as an oral nutraceutical supplement, and has been popular in Asia, Europe, and United States for many years. In this study, a potential and sustainable source of ChS from jumbo squid (Dosidicus gigas) cartilage was explored; ultrasound-assisted extraction (UAE) was used to extract ChS from jumbo squid cartilage. The result of mass transfer coefficients based on Fick’s law showed that UAE had higher mass transfer efficacy. The response surface methodology (RSM) combined with Box–Behnken design (BBD) was employed to evaluate the effects of the extraction parameters. The optimal conditions were extraction temperature of 52 °C, extraction time of 46 min, and NaOH concentration of 4.15%. The crude extract was precipitated by 50% ethanol, which obtained a purified ChS with 23.7% yield and 82.3% purity. The purified ChS measured by energy-dispersive X-ray spectroscopy (EDX) had a carbon to sulfur molar ratio of approximately 14:1. The FTIR, 1H, and 13C NMR confirmed jumbo squid ChS were present in the form of chondroitin-4-sulfate and chondroitin-6-sulfate, with a 4S/6S ratio of 1.62. The results of this study provide an efficient process for production and purification of ChS, and are signiﬁcant for the development and utilization of ChS from jumbo squid cartilage in the nutrient food or pharmaceutical industries.

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Isolation, Purification and Structural Characterestics of Chondroitin Sulfate from Smooth hound Cartilage: In vitro Anticoagulant and Antiproliferative Properties

Cited by 46 publications

References 47 publications

Anti-inflammatory and Antioxidant Activity of Peptides From Ethanol-Soluble Hydrolysates of Sturgeon (Acipenser schrenckii) Cartilage

Anti-inflammatory and Antioxidant Activity of Peptides From Ethanol-Soluble Hydrolysates of Sturgeon (Acipenser schrenckii) Cartilage

A further unique chondroitin sulfate from the shrimp Litopenaeus vannamei with antithrombin activity that modulates acute inflammation

Ultrasonic-Assisted Extraction and Structural Characterization of Chondroitin Sulfate Derived from Jumbo Squid Cartilage

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