Isolation of novel bovine parainfluenza virus type 5 (bPIV5) and its incidence in Korean cattle

Yang, Dong Kun; Nah, Jin Ju; Kim, Ha Hyun; Choi, Sung Suk; Bae, You-Chan; Song, Jae Young

doi:10.14405/kjvr.2014.54.2.107

Cited by 9 publications

(9 citation statements)

References 16 publications

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“…Prior to infection, young adult ferrets (≤1 year of age) were divided into two groups ( n = 6/group): those in the infected group were placed in an isolator. Group I was inoculated with purified virus at a dose of 0.5 ml 10 7.0 TCID 50 /ml (0.2 ml for each nasal passage and 0.05 ml for each eye) of PIV5 as described previously (Chen et al., 2012; Lee et al., 2013; Yang et al., 2014). Tick/HLJ/2019 was diluted in PBS via the intranasal and ocular routes.…”

Section: Methodsmentioning

confidence: 99%

Isolation, identification and pathogenic characteristics of tick‐derived parainfluenza virus 5 in northeast China

Yang

Jiang

et al. 2022

Transbounding Emerging Dis

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The number of parainfluenza virus 5 (PIV5) infection cases has increased worldwide over the past six decades; however, factors underlying this increase remain unclear. PIV5 has been emerging or re‐emerging in humans and animal species. To date, no information is yet available regarding PIV5 infection in arthropod ticks. Here, we successfully isolated tick‐derived PIV5 from the Ixodes persulcatus species designated as HLJ/Tick/2019 in Heilongjiang, China. Phylogenetic analysis revealed that the tick‐derived PIV5 is closely related to subclade 2.2.6, which has become the dominant subtype prevalent in dogs, pigs and wildlife across China. Further experiments to understand the importance of this virus as an infectious vector revealed that a ferret animal model experimentally infected with Tick/HLJ/2019 via the oronasal and ocular inoculation routes developed moderate respiratory distress with pneumonia and neurologic tissue damage from inflammation for the first time. Further surveillance of PIV5 in vectors of viral transmission is necessary to enhance our knowledge of its ecology in reservoirs and facilitate the control of re‐emerging diseases.

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Section: Methodsmentioning

confidence: 99%

Isolation, identification and pathogenic characteristics of tick‐derived parainfluenza virus 5 in northeast China

Yang

Jiang

et al. 2022

Transbounding Emerging Dis

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“…The hemagglutination (HA) test was performed by preparing serial two-fold dilutions of CPIV-5 in 50 µL of PBS (pH 7.2) and adding 50 µL of 0.6% chicken erythrocytes. The HI test was performed in 96-well microplates as described previously [1,23]. The HI titer was expressed as the reciprocal of the highest dilution of serum showing complete inhibition of HA.…”

Section: Virus Neutralization Of Cadv-2mentioning

confidence: 99%

Detection of viral infections in wild Korean raccoon dogs (Nyctereutes procyonoides koreensis)

Yang¹,

Lee²,

Kim³

et al. 2017

Korean J Vet Res

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Wild raccoon dogs (Nyctereutes procyonoides koreensis) may play a role transmitting several pathogens to humans and pet animals. Information concerning the incidence of rabies, canine distemper virus (CDV), canine parvovirus (CPV), canine adenovirus type 2 (CAdV-2), canine parainfluenza virus type 5 (CPIV-5), and canine herpesvirus (CHV) is needed in wild raccoon dogs. In total, 62 brain samples of raccoon dogs were examined for rabies virus (RABV) and CDV, and 49 lung samples were screened for CDV, CAdV-2, CPIV-5, and CHV. No RABV, CAdV-2, CPIV-5, or CHV was identified, but nine CDV antigens (8.1%, 9/111) were detected. Moreover, 174 serum samples from wild raccoon dogs were screened for antibodies against the five major viral pathogens. The overall serosurveillance against CDV, CPV, CAdV-2, CPIV-5, and CHV in wild raccoon dogs was 60.3%, 52.9%, 59.8%, 23.6%, and 10.3%, respectively. Comparisons of the sero-surveillance of the five pathogens showed that raccoon dogs of Gyeonggi province have slightly higher sero-positive rates against CDV, CPV, and CHV than those of Gangwon province. These results indicate high incidences of CDV, CPV, and CAdV-2 in wild raccoon dogs of two Korean provinces and a latent risk of pathogen transmission to companion and domestic animals.

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“…Currently, diagnosis of PIV5 infection is generally based on the detection of nucleic acid by PCR and confirmed by virus isolation (Charoenkul et al., 2021; Yang et al., 2014). Although direct detection of the presence of viral RNA by PCR is sensitive, ELISA test can be more simple, less expensive, and applicable to wider field‐based applications.…”

Section: Introductionmentioning

confidence: 99%

Characterization of parainfluenza virus 5 from diarrheic piglet highlights its zoonotic potential

Ibrahim

Zhang

Werid

et al. 2022

Transbounding Emerging Dis

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Parainfluenza virus 5 (PIV5), a member of paramyxoviruses, causes respiratory and neurological infection in several animal species. Whereas information on PIV5 infection in digestive system is very scarce. Here, we successfully isolated one PIV5 strain from diarrheic piglets. After four times plaque purification and ultracentrifugation, the paramyxovirus‐like particles were observed by electron microscopy. The genome‐wide phylogenetic analysis showed that the isolated strain was closely related to the PIV5 strain from a lesser panda and pigs in China. Therefore, we characterized this isolated PIV5 and found that this virus could haemagglutinate red blood cells from both guinea pigs and chickens. Further, we observed that this PIV5 could infect cell lines from various host species including pig, human, monkey, bovine, dog, cat, rabbit, hamster and mouse, which was confirmed with the immunofluorescent assay. To evaluate the distribution of PIV5 in the field, we developed an indirect ELISA (iELISA) for the first time to detect the specific antibodies based on recombinant nucleocapsid protein. A total of 530 porcine serum samples were tested and the PIV5‐positive rate was 75.7%. To our knowledge, this is the first report describing the full characterization of PIV5 strain isolated from a diarrheic piglet. The ability of this PIV5 strain to infect a wide range of mammalian cell types indicates that PIV5 can transmit across different species, providing a remarkable insight into potential zoonosis. The virus strain and iELISA developed in this study can be used to investigate the pathogenesis, epidemiology, and zoonotic potential of PIV5.

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Isolation of novel bovine parainfluenza virus type 5 (bPIV5) and its incidence in Korean cattle

Cited by 9 publications

References 16 publications

Isolation, identification and pathogenic characteristics of tick‐derived parainfluenza virus 5 in northeast China

Isolation, identification and pathogenic characteristics of tick‐derived parainfluenza virus 5 in northeast China

Detection of viral infections in wild Korean raccoon dogs (Nyctereutes procyonoides koreensis)

Characterization of parainfluenza virus 5 from diarrheic piglet highlights its zoonotic potential

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