Isolation, identification and pathogenic characteristics of tick‐derived parainfluenza virus 5 in northeast China

Yang, Mingfa; Ma, Yunyun; Jiang, Qian; Song, Mingxin; Kang, Huifang; Liu, Jiasen; Qu, Liandong

doi:10.1111/tbed.14681

Cited by 5 publications

(5 citation statements)

References 39 publications

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“…PIV5 of the family Paramyxoviridae could cause disease in humans and animals ( Wang et al, 2023 ). The detection of PIV5 in arthropods had not been documented until it was found in ticks from Jilin Province ( Yang et al, 2022 ). LCMV of the family Arenaviridae was a group of rodent-borne viruses that could cause neurological disorders in humans ( Barton et al, 2002 ).…”

Section: Resultsmentioning

confidence: 99%

Diversity of species and geographic distribution of tick-borne viruses in China

Wu,

Zhou,

Mao

et al. 2024

Front. Microbiol.

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IntroductionTick-borne pathogens especially viruses are continuously appearing worldwide, which have caused severe public health threats. Understanding the species, distribution and epidemiological trends of tick-borne viruses (TBVs) is essential for disease surveillance and control.MethodsIn this study, the data on TBVs and the distribution of ticks in China were collected from databases and literature. The geographic distribution of TBVs in China was mapped based on geographic locations of viruses where they were prevalent or they were detected in vector ticks. TBVs sequences were collected from The National Center for Biotechnology Information and used to structure the phylogenetic tree.ResultsEighteen TBVs from eight genera of five families were prevalent in China. Five genera of ticks played an important role in the transmission of TBVs in China. According to phylogenetic analysis, some new viral genotypes, such as the Dabieshan tick virus (DTV) strain detected in Liaoning Province and the JMTV strain detected in Heilongjiang Province existed in China.DiscussionTBVs were widely distributed but the specific ranges of viruses from different families still varied in China. Seven TBVs belonging to the genus Orthonairovirus of the family Nairoviridae such as Nairobi sheep disease virus (NSDV) clustered in the Xinjiang Uygur Autonomous Region (XUAR) and northeastern areas of China. All viruses of the family Phenuiviridae except Severe fever with thrombocytopenia syndrome virus (SFTSV) were novel viruses that appeared in the last few years, such as Guertu virus (GTV) and Tacheng tick virus 2 (TcTV-2). They were mainly distributed in the central plains of China. Jingmen tick virus (JMTV) was distributed in at least fourteen provinces and had been detected in more than ten species of tick such as Rhipicephalus microplus and Haemaphysalis longicornis, which had the widest distribution and the largest number of vector ticks among all TBVs. Parainfluenza virus 5 (PIV5) and Lymphatic choriomeningitis virus (LCMV) were two potential TBVs in Northeast China that could cause serious diseases in humans or animals. Ixodes persulcatus carried the highest number of TBVs, followed by Dermacentor nuttalli and H. longicornis. They could carry as many as ten TBVs. Three strains of Tick-borne encephalitis (TBEV) from Inner Mongolia Province clustered with ones from Russia, Japan and Heilongjiang Province, respectively. Several SFTSV strains from Zhejiang Province clustered with strains from Korea and Japan. Specific surveillance of dominant TBVs should be established in different areas in China.

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Section: Resultsmentioning

confidence: 99%

Diversity of species and geographic distribution of tick-borne viruses in China

Wu,

Zhou,

Mao

et al. 2024

Front. Microbiol.

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“…As no isolate of K. kristinae has been previously reported in marine fish previously, evaluation of whether this new bacterial isolate could cause infection or death of large yellow croakers was undertaken according to the method for animal regressive infection ( Sibinga and Marquis, 2021 ; Yang et al, 2022 ). First, we streaked the reserved bacterial solution onto a BHI agar plate to obtain a single colony for further cultivation in BHI liquid medium at 37°C for 12 h at 180 rpm; then, we performed an extra inoculation from the obtained bacterial solution, which was at the logarithmic growth phase with an OD600.0 value of 1.874, at a ratio of 1:100 to prepare the bacterial culture.…”

Section: Methodsmentioning

confidence: 99%

A new pathogenic isolate of Kocuria kristinae identified for the first time in the marine fish Larimichthys crocea

et al. 2023

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In recent years, new emerging pathogenic microorganisms have frequently appeared in animals, including marine fish, possibly due to climate change, anthropogenic activities, and even cross-species transmission of pathogenic microorganisms among animals or between animals and humans, which poses a serious issue for preventive medicine. In this study, a bacterium was clearly characterized among 64 isolates from the gills of diseased large yellow croaker Larimichthys crocea that were raised in marine aquaculture. This strain was identified as K. kristinae by biochemical tests with a VITEK 2.0 analysis system and 16S rRNA sequencing and named K. kristinae_LC. The potential genes that might encode virulence-factors were widely screened through sequence analysis of the whole genome of K. kristinae_LC. Many genes involved in the two-component system and drug-resistance were also annotated. In addition, 104 unique genes in K. kristinae_LC were identified by pan genome analysis with the genomes of this strain from five different origins (woodpecker, medical resource, environment, and marine sponge reef) and the analysis results demonstrated that their predicted functions might be associated with adaptation to living conditions such as higher salinity, complex marine biomes, and low temperature. A significant difference in genomic organization was found among the K. kristinae strains that might be related to their hosts living in different environments. The animal regression test for this new bacterial isolate was carried out using L. crocea, and the results showed that this bacterium could cause the death of L. crocea and that the fish mortality was dose-dependent within 5 days post infection, indicating the pathogenicity of K. kristinae_LC to marine fish. Since K. kristinae has been reported as a pathogen for humans and bovines, in our study, we revealed a new isolate of K. kristinae_LC from marine fish for the first time, suggesting the potentiality of cross-species transmission among animals or from marine animals to humans, from which we would gain insight to help in future public prevention strategies for new emerging pathogens.

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“…Particularly, PVs exhibit a wide range of common hosts, suggesting a heightened potential for interspecies transmission. The role of the tick as an intermediate host has been proposed, promoting the transmission of PVs among mammals ( 17 ). Nonetheless, the evolutionary dynamics of PVs during interspecies transmission remain inadequately studied.…”

Section: Introductionmentioning

confidence: 99%

Isolation, genome analysis and comparison of a novel parainfluenza virus 5 from a Siberian tiger (Panthera tigris)

Zhou,

Chen,

Wang

et al. 2024

Front. Vet. Sci.

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Paramyxoviruses are important pathogens affecting various animals, including mammals and humans. Parainfluenza virus 5 (PIV5)—a member of the family Paramyxoviridae—is a major threat to the health of mammals and humans. However, studies on terrestrial wild animals infected with PIV5 are scanty. In this study, we utilized reverse transcription PCR to detect PIV5 infection in the visceral organ tissues of a Siberian tiger (Panthera tigris ssp. altaica) with vomiting, diarrhea, and dyspnea before its death. A novel PIV5 (named SR strain) with a slowly progressive cytopathic effect was isolated in Vero cells and validated using a transmission electron microscope. Full-length sequencing and analysis revealed that the whole genome of the PIV5 SR strain contained 15,246 nucleotides (nt) and seven non-overlapping genes (3’-N-V/P-M-F-SH-HN-L-5′) encoding eight proteins. Phylogenetic analysis of three PIV5 strains identified in the same zoo confirmed that PIV5 strains SR and ZJQ-221 shared the closest genetic relationship as they were clustered in the same branch, while the recently found Siberian tiger strain SZ2 kept a certain distance and formed a relatively unique branch. Furthermore, mutations of nt and amino acids (aa) between strains ZJQ-221, SR, and SZ2 were identified. In summary, we report the identification and genomic characterization of a novel PIV5 strain SR isolated in a Siberian tiger, which may help future research on interspecific transmission mechanisms.

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Isolation, identification and pathogenic characteristics of tick‐derived parainfluenza virus 5 in northeast China

Cited by 5 publications

References 39 publications

Diversity of species and geographic distribution of tick-borne viruses in China

Diversity of species and geographic distribution of tick-borne viruses in China

A new pathogenic isolate of Kocuria kristinae identified for the first time in the marine fish Larimichthys crocea

Isolation, genome analysis and comparison of a novel parainfluenza virus 5 from a Siberian tiger (Panthera tigris)

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