Isolation of multi-drug resistant Klebsiella sp. from bovine mastitis samples in Rangpur, Bangladesh

Abstract

Objective:The objective of this study was to identify the multi-drug resistance (MDR) Klebsiella sp. from mastitis milk samples.Materials and Methods:In the current research, 48 clinical mastitis milk samples were collected from Rangpur division, Bangladesh. Confirmation of bovine mastitis (BM) was done by the California Mastitis Test (CMT). All the CMT positive isolates were subjected for the identification of Klebsiella sp. using through a series of cultural and biochemical tests. MDR Klebsiella sp. isolates were determined using the disk diffusion method, and minimum inhibitory zones were measured by following Clinical and Laboratory Standards Institute. MDR patterns of the isolates were also subjected to study by using housefly (Musca domestica).Results:Among the isolates, 62.5% (n = 30/48) revealed the presence of Klebsiella sp. Eight antimicrobial agents including Amoxicillin, Novobiocin, Erythromycin, Vancomycin, Cephradine, Tetracycline, Bacitracin, Methicillin, and housefly (M. domestica) showed complete resistance to Klebsiella sp. On the other hand, Chloramphenicol, Gentamicin, Ciprofloxacin, Azithromycin, Norfloxacin, Levofloxacin, and Nalidixic acid showed sensitivity.Conclusion:This study helps to treat BM with effective antibiotics and helps in an epidemiological study in Rangpur division as well as helps to create public health awareness.

Multidrug resistant Klebsiella Pneumoniae reservoir and their capsular resistance genes in cow farms of district Peshawar, Pakistan

“…pneumoniae was found at 44.4% with a MAR range from 0.21–0.92. These results are in agreement with previous studies of Salauddin et al [ 19 ], who reported a 62.5% incidence of MDR K . pneumoniae and Osman et al [ 18 ] reported 40%, while in contrast to our study, the Yang et al [ 25 ] reported 9.78% MDR K .…”

“…In the current study, MDR K. pneumoniae was found at 44.4% with a MAR range from 0.21-0.92. These results are in agreement with previous studies of Salauddin et al [19], who reported a 62.5% incidence of MDR K. pneumoniae and Osman et al [18] reported 40%, while in contrast to our study, the Yang et al [25] reported 9.78% MDR K. pneumoniae. The present study antibiogram analysis of cow milk isolated MDR K. pneumoniae showed high resistance…”

“…In Bangladesh, Klebsiella spp. was found in 62.5% of mastitic milk [ 19 ] while in India found 20% to 24% in different studies [ 20 , 21 ]. In the present study, among the total 700 collected milk samples of cows, K .…”

“…The pace of MDR Klebsiella spp., growth is accelerating daily. Globally, concerns including the rise of MDR bacteria, the use of antibiotics to treat them, and zoonotic diseases are considered critical [ 19 ]. The MAR index is determined by dividing the number of antibiotic isolates that are resistant by the total number of antibiotics the organism has been to expose [ 17 ].…”

Incidence, Etiology, and Risk Factors of Clinical Mastitis in Dairy Cows under Semi-Tropical Circumstances in Chattogram, Bangladesh

“…dysgalactiae have been found to be either contagious or environmental [ 18 , 23 , 24 , 25 ]. As far as the authors are aware, bacterial agent results from previous studies in Bangladesh have only been reported from subclinical mastitis (SCM) [ 26 , 27 ].…”