Isolation of Rickettsia rickettsii and Rickettsia bellii in Cell Culture from the Tick Amblyomma aureolatum in Brazil

Pintér, Adriano; Labruna, Marcelo Bahia

doi:10.1196/annals.1374.103

Cited by 180 publications

(185 citation statements)

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“…A. aureolatum is another involved tick in the epidemiology of BSF, feeding on wild animals during it immature stages and parasitizing dogs during the adult stage. R. rickettsii was identified in this tick, collected from dogs at an endemic region of São Paulo state, suggesting be an important vector in Brazil (Pinter & Labruna 2006). Dogs from rural areas move freely in pastures, forests and stables around the houses, most of them being pets, mainly for children.…”

Section: Resultsmentioning

confidence: 89%

First identification of natural infection of Rickettsia rickettsii in the Rhipicephalus sanguineus tick, in the State of Rio de Janeiro

et al. 2009

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The Brazilian Spotted Fever (BSF) is a zoonotic disease caused by Rickettsia rickettsii and transmitted by ticks of the genus Amblyomma, more frequently, Amblyomma cajennense. The aim of this paper was to report the first molecular detection of R. rickettsii on R. sanguineus naturally infected in Rio de Janeiro, Brazil. Ticks were collected from dogs in a rural region of Resende municipality, Rio de Janeiro State, Brazil (22º30'9.46"S, 44º42'44.29"WO), where occurred five human cases of BSF in 2006. The ticks were identified under a stereoscopic microscope and separated in pools by stages, species and sex. DNA extraction was carried out using QIAamp DNA Mini Kit (QIAGEN®). The DNA was submitted to PCR amplification using 04 set of primers: Rr190.70p/Rr190.602n (OmpA, 532bp), BG1-21/BG2-20 (OmpB, 650bp), Tz15/Tz16 (17 kDa protein-encoding gene, 246bp) and RpCS.877p/RpCS.1258n (gltA, 381bp). PCR products were separated by electrophoresis on 1% agarose gels and visualized under ultraviolet light with ethidium bromide. PCR products of the expected sizes were purified by QIAquick® and sequenced by ABI PRISM®. The generated nucleotide sequences were edited with using Bioedit® software and compared with the corresponding homologous sequences available through GenBank, using Discontiguous Mega Blast (http://www.ncbi.nlm.nih.gov). It was confirmed R. rickettsii by sequencing of the material (GenBank FJ356230). The molecular characterization of R. rickettsii in the tick R. sanguineus emphasizes the role of dogs as carriers of ticks from the environment to home. Moreover, this result suggests that there is a considerable chance for active participation of R. sanguineus as one of tick species in the transmission of R. ricketsii to human being in the Brazilian territory.

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Section: Resultsmentioning

confidence: 89%

First identification of natural infection of Rickettsia rickettsii in the Rhipicephalus sanguineus tick, in the State of Rio de Janeiro

et al. 2009

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“…Já quanto a R. bellii, atualmente a riquétsia mais prevalente em carrapatos no Brasil (15,16) , não há evidência que comprove a patogenicidade dessa bactéria para humanos ou animais (18) . Das 30 amostras de soro colhidas de humanos, residentes em meio urbano do município de Quirinópolis, 50% (15/30) reagiram para algum tipo de antígeno riquétsial (Tabela 4).…”

Section: Resultsunclassified

“…Essas cepas foram isoladas de carrapatos do Brasil, conforme trabalhos de Horta et al (14) , Labruna et al (15) e Pinter e Labruna (16) . Para obtenção dos antígenos, foram utilizados cultivos de células Vero infectadas com Rickettsia spp.…”

Section: Todas As Amostras De Sangue Obtidas Foram Centrifugadas No Lunclassified

Inquérito Epidemiológico De Suposto Foco De Febre Maculosa

et al. 2016

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Resumo O presente estudo objetivou avaliar a suspeita de ocorrência da febre maculosa (FM) em Quirinópolis - GO, por meio de inquérito sorológico em amostras de humanos, cães e equinos e identificação de carrapatos vetores. Em 2007, foram realizadas reações de imunofluorescência indireta (RIFI) para Rickettsia spp. em amostras de sangue colhidas de 28 pessoas do grupo de convívio de um caso suspeito (uma criança de três anos que apresentou sinais de riquetsiose, após exposição a carrapatos). Também foram colhidas amostras de sangue em cinco cães e 42 equinos, havendo soro reagentes somente entre os equinos (28,6%). Não foi constatada a presença do carrapato Amblyomma cajennense. Sete indivíduos foram reagentes para Rickettsia spp., a maioria desses eram parentes paternos do caso suspeito. Em 2008, na mesma região, foram realizadas RIFI em amostras de 30 humanos sem sinais clínicos de FM e constatou-se 50% de soro reagentes para Rickettsia spp. Adicionalmente, colheram-se amostras de sangue de 24 cães errantes, havendo um cão soropositivo. A evidência sorológica do contato entre bactérias do gênero Rickettsia com habitantes humanos e animais de Quirinópolis indica que a FM ou outra riquetsiose possa estar ocorrendo no Estado de Goiás.

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“…In one case, R. rickettsii was isolated by inoculation of clinical samples in guinea pigs (Melles et al 1992). Both R. rickettsii and R. bellii have been isolated from ticks, and A. aureolatum was incriminated as the vector of BSF (Pinter & Labruna 2006). In addition, R. felis has been reported in fleas C. felis felis ).…”

Section: Methodsmentioning

confidence: 99%

“…Capybaras, horses, as well as A. cajennense and A. dubitatum are rarely found in this area (Adriano Pinter et al unpublished observations). Samples were collected in the Taiaçupeba district, the same area sampled by Pinter and Labruna (2006). Endemic area 3: Piracicaba (22 o 42´22.8"S, 47 o 37´39.4"W).…”

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confidence: 99%

Rickettsia infection in five areas of the state of São Paulo, Brazil

Horta

Labruna

Pintér

et al. 2007

Mem. Inst. Oswaldo Cruz

178

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This study investigated rickettsial infection in animals, humans, ticks, and fleas collected in five areas of the state of São Paulo. Eight flea species (Adoratopsylla antiquorum antiquorum, Ctenocephalides felis felis, Polygenis atopus, Polygenis rimatus, Polygenis roberti roberti, Polygenis tripus, Rhopalopsyllus lugubris, and Rhopalopsyllus lutzi lutzi), and five tick species (Amblyomma aureolatum, Amblyomma cajennense, Amblyomma dubitatum, Ixodes loricatus, and Rhipicephalus sanguineus) were collected from dogs, cats, and opossums. Rickettsia felis was the only rickettsia found infecting fleas, whereas Rickettsia bellii was the only agent infecting ticks, but no animal or human blood was shown to contain rickettsial DNA. Testing animal and human sera by indirect immunofluorescence assay against four rickettsia antigens (R. rickettsii, R. parkeri, R. felis, and R. bellii), some opossum, dog, horse, and human sera reacted to R. rickettsii with titers at least four-fold higher than to the other three rickettsial antigens. These sera were considered to have a predominant antibody response to R. rickettsii. Using the same criteria, opossum, dog, and horse sera showed predominant antibody response to R. parkeri or a very closely related genotype. Our serological results suggest that both R. rickettsii and R. parkeri infected animals and/or humans in the studied areas.Key words: Rickettsia -spotted fever -fleas -ticks -opossum -domestic animals Rickettsiae are obligate intracellular bacteria that have been classically divided into two groups: the typhus group (TG), composed of Rickettsia prowazekii and Rickettsia typhi, which are associated with lice and fleas, respectively; and the spotted fever group (SFG), which includes more than 20 valid species, mostly associated with ticks (e.g., Rickettsia rickettsii, Rickettsia parkeri), and at least one species associated with fleas (Rickettsia felis). Other species, such as Rickettsia bellii and Rickettsia canadensis, both associated with ticks, have been considered a distinct group sharing antigenic, genetic and ecological characters with both TG and SFG rickettsiae (Parola et al. 2005b). Since all SFG Rickettsia share common outer membrane antigens, conventional serological assays employing a single Rickettsia antigen (e.g., R. rickettsii) allow detecting a SFG infection, but they do not identify the Rickettsia species responsible for the infection, even in the presence of high titers (La Scola & Raoult 1997). For a more specific serological diagnosis, it is recommended techniques such as testing serum against all known Rickettsia species present in a given area, or serum cross-absorption tests (La Scola & Raoult 1997, Parola et al. 2005b).For many years, SFG rickettsiosis in the Americas was thought to be caused only by R. rickettsii, transmitted by ticks (Weiss & Moulder 1984). This rickettsia has been reported in several countries from North, Central, and South America causing an acute, highly lethal febrile disease receiving different names according to the country...

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Isolation of Rickettsia rickettsii and Rickettsia bellii in Cell Culture from the Tick Amblyomma aureolatum in Brazil

Cited by 180 publications

References 13 publications

First identification of natural infection of Rickettsia rickettsii in the Rhipicephalus sanguineus tick, in the State of Rio de Janeiro

First identification of natural infection of Rickettsia rickettsii in the Rhipicephalus sanguineus tick, in the State of Rio de Janeiro

Inquérito Epidemiológico De Suposto Foco De Febre Maculosa

Rickettsia infection in five areas of the state of São Paulo, Brazil

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