Isolation of<i>Borrelia burgdorferi</i>from the Myocardium of a Patient with Long-Standing Cardiomyopathy

Stanek, Gerold; Klein, Jutta; Bittner, Reginald E.; Glogar, Dietmar

doi:10.1056/nejm199001253220407

Cited by 250 publications

(116 citation statements)

References 13 publications

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“…At 2 wk after inoculation, 1,4,6,8,10, and 12 wk from a site in proximity to the injection site. At 4, 6, 8, 10, and 12 wk, groups of three rabbits were killed for the determination of visceral infection.…”

Section: Resultsmentioning

confidence: 99%

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Rabbit model of Lyme borreliosis: erythema migrans, infection-derived immunity, and identification of Borrelia burgdorferi proteins associated with virulence and protective immunity.

et al. 1995

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Erythema migrans (EM), persistent skin infection, and visceral dissemination can be induced reproducibly in the adult male New Zealand White rabbit by intradermal injection of as few as 103 Borrelia burgdorferL EM was found to persist for 7±3 d. Skin culture positivity (infection) cleared within a mean of 6.7±1.4 wk after infection and similarly visceral infection was not demonstrated after 8 wk; infection-derived immunit to intradermal challenge was evident 5 mo after initial infection. The extent of the protection against EM and dermal infection induced by untreated infection was directly related to

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Section: Resultsmentioning

confidence: 99%

“…Early Lyme disease may also be characterized by spirochetal dissemination with subsequent involvement of joints, central and peripheral nervous systems, lymphoid tissue, and the heart. (2,(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Rabbit model of Lyme borreliosis: erythema migrans, infection-derived immunity, and identification of Borrelia burgdorferi proteins associated with virulence and protective immunity.

et al. 1995

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“…The Borrelia-specific probes described here are FISH on Borrelia-infected Ixodes ricinus ticks well suited to study borreliae in whole ticks and clinical specimens, for example on biopsies of patients with questionable LB as was reported by Stanek et al (1990) on an endomyocardial biopsy specimen. Moreover, because of their good performance in PCR, the newly designed primers could be helpful in establishing a diagnostic assay for LB patients.…”

Section: Discussionmentioning

confidence: 99%

Visualization of Borrelia burgdorferi sensu lato by fluorescence in situ hybridization (FISH) on whole-body sections of Ixodes ricinus ticks and gerbil skin biopsies

et al. 2001

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The objective of this study was to visualize borreliae directly in whole-body sections of Ixodes ricinus by fluorescence in situ hybridization (FISH). Borrelia afzelii mono-infected or Borrelia burgdorferi sensu stricto (ss)/B. afzelii doubleinfected nymphs were fixed, embedded in cold polymerizing resin and sectioned. The same sample processing was applied to skin biopsies taken from a Mongolian gerbil after an infectious tick-bite. FISH was carried out using 16S-rRNA-directed, fluorescence-labelled oligonucleotide probes specific for the genus Borrelia and specific within the group of Lyme borreliosisassociated genospecies B. afzelii, B. burgdorferi ss, Borrelia garinii and Borrelia valaisiana. Sensitivity and specificity of the newly designed probes were evaluated using PCR, dot-blot hybridizations and FISH. Despite significant autofluorescence of certain tick tissues (which allowed good histological orientation within the sections), borreliae showing the typical spirochaetal morphotype were clearly visible in five out of six putatively infected ticks. These findings were confirmed by electron microscopy of ticks from the same infected batch as used for FISH. Attempts to produce ticks infected by two different Borrelia genospecies were not successful. FISH on whole-body sections of resin-embedded ticks offers the possibility of visualizing and identifying borreliae within tick tissues. This technique has great potential for the investigation of the transmission of bacteria or other micro-organisms by arthropod vectors. Furthermore, clear visualization of single spirochaetes distributed along subcutaneous fat cell membranes in gerbil skin biopsies suggests that FISH might also be suitable for the detection of borreliae in clinical tissue specimens.

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“…Lyme carditis can progress to complete heart block and cause sudden death (35). In Europe, Borrelial burgdorferi has been isolated from endomyocardial biopsy samples from several patients with chronic dilated cardiomyopathy (36,37).…”

Section: Extracutaneous Manifestations Of Lyme Diseasementioning

confidence: 99%

Ticks, Borrelia Burgdorferi and Lyme Disease

Bağcı¹,

Ruzicka²

2016

tdd

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Lyme disease, which is caused by spirochete Borrelia burgdorferi sensu lato, is a ticktransmitted, multisystem infectious disease. It occurs in stages, with different clinical manifestations at each stage. Erythema migrans is the most frequent manifestation which occurs at the site of the tick bite. Borrelial lymphocytoma and acrodermatitis chronica atrophicans are late-stage cutaneous manifestations. Extracutaneous signs of infection most often involve the musculoskeletal, cardiovascular, and nervous systems. Serologic assays remain the mainstay of diagnosis. All stages of the disease are curable with appropriate antibiotic therapy. Keywords: Lyme disease, Borrelia burgdorferi, erythema migrans, acrodermatitis chronica atrophicans, ticks, Ixodes Işın Sinem Bağcı, Thomas Ruzicka* Ticks, Borrelia Burgdorferi and Lyme DiseaseKeneler, Borrelia Burgdorferi ve Lyme Hastalığı Öz AbstractLyme hastalığı Borrelia burgdorferi sensu lato grubu spiroketlerin sebep olduğu multisistemik bir enfeksiyon hastalığıdır. Her hastalık evresi değişik klinik bulgularla kendini gösterir. En sık klinik bulgusu kene ısırık yerinde gelişen eritema migranstır. Borrelial lenfositoma ve akrodermatitis kronika atrofikans geç dönemde gelişen deri bulgularıdır. Deri bulguları dışında kas-iskelet, kardiyovasküler ve sinir sistemi tutulumu da görülebilir. Tanı serolojik testler ile konulur. Hastalığın her evresi uygun antibiyotiklerle tedavi edilebilir.

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Isolation ofBorrelia burgdorferifrom the Myocardium of a Patient with Long-Standing Cardiomyopathy

Cited by 250 publications

References 13 publications

Rabbit model of Lyme borreliosis: erythema migrans, infection-derived immunity, and identification of Borrelia burgdorferi proteins associated with virulence and protective immunity.

Rabbit model of Lyme borreliosis: erythema migrans, infection-derived immunity, and identification of Borrelia burgdorferi proteins associated with virulence and protective immunity.

Visualization of Borrelia burgdorferi sensu lato by fluorescence in situ hybridization (FISH) on whole-body sections of Ixodes ricinus ticks and gerbil skin biopsies

Ticks, Borrelia Burgdorferi and Lyme Disease

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