Isolation of high quality DNA: a protocol combining “rennet” and glass milk

Oliveira, Luiz Felipe Valter de; Wallau, Gabriel Luz; Loreto, Élgion Lúcio Silva

doi:10.2225/vol12-issue2-fulltext-4

Cited by 19 publications

(17 citation statements)

References 26 publications

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“…Table 1 also shows the species used as positive and negative controls for copia presence in the molecular assays. Genomic DNA was prepared from adult flies as previously described (Oliveira et al, 2009). PCR analyses were performed in 50 ml reactions using 50 ng of genomic DNA, 1 U of Taq DNA Polymerase (Invitrogen), 1rreaction buffer, 200 mM of NTPs, 20 pmol of each primer and 2 .…”

Section: Methodsmentioning

confidence: 99%

Thecopiaretrotransposon and horizontal transfer inDrosophila willistoni

et al. 2011

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SummaryThe copia element is a retrotransposon that is hypothesized to have been horizontally transferred from Drosophila melanogaster to some populations of Drosophila willistoni in Florida. Here we have used PCR and Southern blots to screen for sequences similar to copia element in South American populations of D. willistoni, as well as in strains previously shown to be carriers of the element. We have not found the canonical copia element in any of these populations. Unlike the P element, which invaded the D. melanogaster genome from D. willistoni and quickly spread worldwide, the canonical copia element appears to have transferred in the opposite direction and has not spread. This may be explained by differences in the requirements for transposition and in the host control of transposition.

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Section: Methodsmentioning

confidence: 99%

Thecopiaretrotransposon and horizontal transfer inDrosophila willistoni

et al. 2011

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“…Genomic DNA was isolated from approximately 100 worms of each strain following the method described by Oliveira et al (2009). As previously highlighted, the strains were made from a single worm.…”

Section: Dna Barcodingmentioning

confidence: 99%

Stenostomum Leucops Dugès, 1828 (Platyhelminthes, Catenulida): A Putative Species Complex With Phenotypic Plasticity

Rosa¹,

Pereira²,

Ragagnin³

et al. 2015

Pap. Avulsos Zool. (São Paulo)

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“…Genomic DNA from 21 Neotropical Drosophila species (Supplementary spreadsheet 1) was prepared following Oliveira et al (2009). P-like transposable element sequences were amplified by PCR using degenerated primers and the PCR conditions described by Lee et al (1999).…”

Section: Identification Of P-like Transposable Element Sequences In Nmentioning

confidence: 99%

New Drosophila P-like elements and reclassification of Drosophila P-elements subfamilies

et al. 2012

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Genomic searches for P-like transposable elements were performed (1) in silico in the 12 available Drosophila genomes and (2) by PCR using degenerate primers in 21 Neotropical Drosophila species. In silico searches revealed P-like sequences only in Drosophila persimilis and Drosophila willistoni. Sixteen new P-like elements were obtained by PCR. These sequences were added to sequences of previously described P-like elements, and a phylogenetic analysis was performed. The subfamilies of P-elements described in the literature (Canonical, M, O, T, and K) were included in the reconstructed tree, and all were monophyletic. However, we suggest that some subfamilies can be enlarged, other subdivided, and some new subfamilies may be proposed, totalizing eleven subfamilies, most of which contain new P-like sequences. Our analyses support the monophyly of P-like elements in Drosophilidae. We suggest that, once these elements need host-specific factors to be mobilizable, the horizontal transfer (HT) of P-like elements may be inhibited among more distant taxa. Nevertheless, HT among Drosophilidae species appears to be a common phenomenon.

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Isolation of high quality DNA: a protocol combining “rennet” and glass milk

Cited by 19 publications

References 26 publications

Thecopiaretrotransposon and horizontal transfer inDrosophila willistoni

Thecopiaretrotransposon and horizontal transfer inDrosophila willistoni

Stenostomum Leucops Dugès, 1828 (Platyhelminthes, Catenulida): A Putative Species Complex With Phenotypic Plasticity

New Drosophila P-like elements and reclassification of Drosophila P-elements subfamilies

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