Isolation of diosmin from plants of the genusVicia andHyssop us officinalis and its influence on blood coagulation

Ивашев, М. Н.; Андреева, О. А.; Bandyukova, V. A.; Dragaleva, T. D.

doi:10.1007/bf02219532

Cited by 8 publications

(5 citation statements)

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“…The obtained sediments were washed several times with ultrapure water and centrifuged at 3000 rpm for 15 min. The acquired sediments were dissolved in a mixture of DMSO-methanol (1:1, v/v) and applied for purification by a preparative HPLC system [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

Qualitative and quantitative analysis of diosmin content of hyssop (Hyssopus officinalis) in response to salinity stress

Soheilikhah

Modarresi

Karimi

et al. 2021

Heliyon

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Hyssop (Hyssopus officinalis L.) is a perennial subshrub, which is distributed across the eastern Mediterranean region to central Asia. One of the most important bioactive compounds of hyssop is diosmin, a flavone glycoside of diosmetin, with application in the field of cardiovascular therapy. Salinity as one of the most essential environmental stress factors is able to alter secondary metabolite content in plants. Therefore, we aimed to investigate the effect of salinity on the levels of total flavonoid content and diosmin in hyssop. Accordingly, salinity stress was imposed by watering plants with four different concentrations of sodium chloride (NaCl) (50, 100, 150 and 200 mM) for 4 weeks. High-performance liquid chromatography (HPLC) method was used for purification of diosmin from dried leaves and measurement of it in dried shoots. Nuclear magnetic resonance (NMR) spectroscopy was applied for determination of the structure of diosmin. The obtained results showed that high salinity levels lead to a higher amount of total flavonoid and diosmin content in treated plants. Although alteration in diosmin content was not significant in treatments up to 100 mM NaCl, higher amounts of diosmin were observed in 150 and 200 mM NaCl salinity levels. We concluded that the contents of total flavonoid and diosmin were significantly elevated after exposure of hyssop plants to salt stress conditions.

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Section: Methodsmentioning

confidence: 99%

Qualitative and quantitative analysis of diosmin content of hyssop (Hyssopus officinalis) in response to salinity stress

Soheilikhah

Modarresi

Karimi

et al. 2021

Heliyon

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“…The early work of Ivashev, Andreeva, Bandyukova, and Dragaleva (1995), proved that Asian vetch contained diosmin in sufficient amount suitable for industrial production and showed that diosmin in small doses (2 and 10 mg/kg) enhances the coagulation time and inhibits fibrinolysis.…”

Section: Pharmacological and Biological Activities Of Vicia Plantsmentioning

confidence: 99%

Vicia plants—A comprehensive review on chemical composition and phytopharmacology

Salehi

Abu‐Reidah

Sharopov

et al. 2020

Phytotherapy Research

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The plants belonging to the genus Vicia are of great interest as a source of many bioactive compounds and micronutrients. A snapshot of their cultivation, habitat, main components, from which essential oils can be obtained, is given. The traditional medicinal uses of Vicia plants are also reported, as well as the wide spectrum of the main biological activities attributed to Vicia plants is discussed regarding potential health beneficial properties, in particular anti‐Parkinson, anticholinesterase, antidepressant, anticonvulsant, antimicrobial, cytotoxic, antioxidant, antiinflammatory and antinociceptive, antidiabetic, antihemolytic, anticoagulant, estrogenic, diuretic, antihypoxic activities.

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“…This procedure makes it possible to separate the target compound rather easily from accompanying flavonoids. According to the method proposed by Ivashev et al [4] plant material (Hyssopus officinalis L., Lamiaceae), after defatting with chloroform, was treated with aqueous ethanol or water (and subsequently with 96% ethanol), and then diosmin was extracted from the plant material with dimethyl sulfoxide DMSO. The extracts obtained were combined and poured into a tenfold amount of water and a crystalline precipitate of diosmin was separated within 48 h. Purification by recrystallization from DMSO-water and DMSO-methanol mixtures and washing with ethanol was suggested to further purify diosmin from accompanying compounds [4].…”

Section: Determination In Plant Extracts and Pharmaceutical Formulatimentioning

confidence: 99%

“…The therapeutic action of diosmin is attributed to the inhibitory effect on capture and metabolism of noradrenalin by the venous walls. This results in an increase in the vascular tonus with favorable consequences, reduction of elasticity and permeability [4]. As a flavonoid, diosmin also exhibits antiinflammatory, free-radical scavenging, and antimutagenic properties [1a,3].…”

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confidence: 99%

Diosmin – Isolation Techniques, Determination in Plant Material and Pharmaceutical Formulations, and Clinical Use

Bogucka‐Kocka

Woźniak

Feldo

et al. 2013

Natural Product Communications

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Diosmin is a naturally occurring flavone glycoside used in the treatment of venous diseases. In this review, we present the clinical aspects of the use of diosmin preparations in venous stasis, hemorrheologic disorders and vein wall remodeling. Because of its multiple applications in biology and its many therapeutic activities, research on isolation and identification of diosmin is of high relevance. The aim of this review is to present an overview of techniques of isolation and separation of diosmin in plant material, pharmaceutical formulations such as Daflon ® , Diosed ® and Dioven ® tablets, and biological fluids.

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Isolation of diosmin from plants of the genusVicia andHyssop us officinalis and its influence on blood coagulation

Cited by 8 publications

References 1 publication

Qualitative and quantitative analysis of diosmin content of hyssop (Hyssopus officinalis) in response to salinity stress

Qualitative and quantitative analysis of diosmin content of hyssop (Hyssopus officinalis) in response to salinity stress

Vicia plants—A comprehensive review on chemical composition and phytopharmacology

Diosmin – Isolation Techniques, Determination in Plant Material and Pharmaceutical Formulations, and Clinical Use

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