Isolation of Dihydroquercetin Glycoside From the Root Bark of Calotropis Procera and Antioxidant and Cytotoxic Screening of the Crude Extracts.

Usman, A.; Mohammad, Rashid; Abdullahi, Aliyu; Zakari, Abdu; Usman, N.L.

doi:10.46602/jcsn.v46i1.576

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on the NMR (1D and 2D) and in comparison with the previously published data, the isolated compounds were identified as yukovanol (1) [34,35], 5,7,4′-trihydroxy-3′-(2-hydroxy-3-methyl-3-butenyl)isoflavone (2) [36], cajanin (3) [37], taxifolin (4) [38], protocatechuic acid (5) [39], saccharose (6) [40], and stigmasterol (7) [41], (Fig. 2).…”

Section: Resultsmentioning

confidence: 90%

Chemical Constituents from the Stem Bark of Ficus thonningii and their Chemotaxonomic Significance

Ibrahim

Omosa

Onyari

et al. 2022

EJMP

View full text Add to dashboard Cite

Background of the Study: Tropical plants of the Ficus genus (Moraceae) are among the earliest fruit trees that humans have cultivated. Since ancient times, many folk medicines have used species of this genus to treat a variety of ailments. Evidence from earlier investigations has shown these plants contain abundant secondary metabolites with a variety of structural properties and biological functions. Place and Duration of Study: The research was carried out at the University of Nairobi (Faculty of Science and Technology, Department of Chemistry) from January to June 2022. Aim: The study focuses on isolating and identifying secondary metabolites from the stem bark of Ficus thonningii Blume found in Kenya and their chemotaxonomic significance. Methodology: Dried powdered stem bark of Ficus thonningii was extracted by maceration at room temperature using CH2Cl2/CH3OH (1:1) to yield a crude extract which was fractionated in a chromatographic column (CC) using silica gel (60 – 120 mesh) as an adsorbent eluting with EtOAc/n-hexane followed by CH3OH/EtOAc. The fractions were purified using silica gel (70 – 230, 230 – 400 mesh) CC and chromatotron eluting with solvents of different polarity, as well as a crystallization technique. Structures of the isolated compounds were elucidated and identified using the spectroscopic method (NMR (1D and 2D)) and by comparison with reported literature data. Results: Phytochemical investigation of the stem bark of Ficus thonningii afforded seven compounds, including yukovanol (1), 5,7,4′-trihydroxy-3′-(2-hydroxy-3-methyl-3-butenyl)isoflavone (2), cajanin (3), taxifolin (4), protocatechuic acid (5), saccharose (6), and stigmasterol (7). Compounds 1 - 3, 5 and 7 were not reported from F. thonningii until now. Further, compound 6 is being isolated from the genus Ficus for the first time. Conclusion: The chemotaxonomic significance of the isolated phytochemicals demonstrates the taxonomic position of F. thonningii in the genus Ficus and explains its multiple ethnomedicinal applications.

show abstract

Section: Resultsmentioning

confidence: 90%

Chemical Constituents from the Stem Bark of Ficus thonningii and their Chemotaxonomic Significance

Ibrahim

Omosa

Onyari

et al. 2022

EJMP

View full text Add to dashboard Cite

show abstract

“…Te antioxidant activity of these compounds depends on the molecular structure, in particular on the number and position of hydroxyl groups and the nature of substitutions on the aromatic rings [49]. Some favonoids such as rutin, isorhamnetin, and dihydroquercetin have been reported from C. procera root bark [50,51] and have efcient antioxidant activity [52][53][54]. Terefore, the observed DPPH + scavenging activity may probably be due to the hydrogendonating capability of the polyphenolic compounds present in EAF and DMF [55].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Analysis, In Vitro Free Radical Scavenging, and LDL Protective Effects of Different Solvent Fractions of Calotropis procera (R.) Br. Root Bark Extract

Kyada,

Rahamathulla,

Santani

et al. 2023

Journal of Food Biochemistry

View full text Add to dashboard Cite

In the present study, the methanolic extract of Calotropis procera root bark was subjected to solvent-solvent partitioning using n-hexane fraction (HF), dichloromethane fraction (DMF), ethyl acetate fraction (EAF), and methanol fraction (MF). The resultant fractions were tested for antioxidant activity using in vitro radical scavenging assay. Preliminary phytochemical investigation revealed the presence of varying proportions of secondary metabolites in solvent fractions such as glycosides, flavonoids, triterpenoids, sterols, and polyphenolic compounds. The total phenolic content of EAF was 25.7 ± 3.12 mg TAE/g followed by 19.05 ± 3.29 mg TAE/g in DMF. The total flavonoid content was 13.69 ± 1.74 mg QUE/g in DMF and 11.4 ± 1.88 mg QUE/g in EAF. The EAF showed significant radical-scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH, IC50 = 369.87 μg/mL), nitric oxide (IC50 = 317.46 μg/mL), hydrogen peroxide (H2O2 , IC50 = 396.85 μg/mL), and hydroxyl radicals (IC50 = 195.39 μg/mL). DMF was most effective in scavenging superoxide radicals (IC50 = 679.60 μg/mL), while greater metal chelating activity was exhibited by MF (IC50 = 614.73 μg/mL). Moreover, the total antioxidant activity for EAF was found to be 94.14 ± 9.114 mg AAE followed by DMF (68.10 ± 8.78 mg AAE). EAF also significantly reduced the formation of thiobarbituric acid reactive substances in a dose-dependent manner in CuSO4. The observed antioxidant effect might be attributed to the presence of secondary metabolites. Subsequent GC-MS analysis of EAF confirmed the presence of lupeol, α-amyrin, β-amyrin, and ursolic acid. The current investigation reveals that the high polyphenolic and antioxidant pentacyclic triterpenes in EAF of C. procera root bark methanol extract correlates with its good antioxidant activity and can provide protection against free radicals-induced damage in a variety of chronic health conditions.

show abstract

A systematic review on the biological evaluation of Calotropis procera (Aiton) Dryand

Abdulrahman

2023

Futur J Pharm Sci

View full text Add to dashboard Cite

Background Humans have used plants as a safe and effective medicine for a wide range of ailments ever since the earliest days of civilization. Calotropis procera potential as a treatment for a variety of ailments has been known for quite some time. This xerophytic, upright shrub grows to a height of about 6 m and can be found in the tropics of Africa and Asia. Its parts have been used to cure a variety of ailments, including rheumatism, fever, dysentery, diabetes, malaria, asthma, and many more. Here, we provide a synopsis of the available biological data and discuss the possible ways in which Calotropis procera could be used as a novel platform for the treatment of a wide range of diseases. Main body High antioxidant, antiinflammatory, antianalgesic, antimicrobial, antimalaria, antidiabetic, wound-healing, hepato-protective, nerve-recovery, antiulcer, insecticidal, and anticancer effects have been observed in the latex. The research also found that excessive intake has negative health effects. Conclusion The review discovered that the biological evaluation of C. procera in vitro and in vivo animal models was well documented. Human safety and efficacy, however, have yet to be thoroughly tested, and additional well-designed clinical trials are required to confirm preclinical findings. It is essential to establish a standard dose and assure its safety.

show abstract

Isolation of Dihydroquercetin Glycoside From the Root Bark of Calotropis Procera and Antioxidant and Cytotoxic Screening of the Crude Extracts.

Cited by 3 publications

References 0 publications

Chemical Constituents from the Stem Bark of Ficus thonningii and their Chemotaxonomic Significance

Chemical Constituents from the Stem Bark of Ficus thonningii and their Chemotaxonomic Significance

Phytochemical Analysis, In Vitro Free Radical Scavenging, and LDL Protective Effects of Different Solvent Fractions of Calotropis procera (R.) Br. Root Bark Extract

A systematic review on the biological evaluation of Calotropis procera (Aiton) Dryand

Contact Info

Product

Resources

About