The root bark of Calotropis procera (Family: Asclepiadaceae) was extracted with methanol. The methanolic extract was separated into hexane, chloroform, ethyl acetate, and water soluble fractions. The ethyl acetate fraction was subjected to bioassay-guided fractionation and final purification was achieved by column chromatography. The structure of the compound was elucidated by spectroscopic methods (ESI-MS, 1H and C NMR, COSY, HSQC, and HMBC) and the comparison of the data obtained with that reported in the literature. It was concluded that the compounds isolated was taxifolin 4’-O-β-D-glucopyranoside, a dihydroquercetin glycoside. The crude extracts of hexane, chloroform, ethylacetate, and water soluble fractions of methanol extract and the isolated compound were subjected to antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and brine shrimp lethality bioassay. The result shows that the maximum inhibitions in the DPPH assays were isolated compound (51.7 %), methanol (80.7 %), and water (29.2 %) fractions while ascorbic acid standard was (84.4 %). However, the results for hexane, chloroform and ethyl acetate fractions in the DPPH assays were poor and hence discarded. The results for the in vitro cytotoxicity activity shows that ethyl acetate and hexane fractions showed significant cytotoxicity with LC50 value of 1.0±0.2 and 2.4 ±0.1 respectively.
Green Chemistry is the plan of synthetic items and cycles that lessen or wipe out the utilization and age of substances risky to people, creatures, plants and the climate. It is an advanced science that arrangements with the use of natural agreeable synthetic mixtures and materials in the different parts of our life like modern uses and numerous others. The start of green science is considered as a reaction to the need to diminish the harm of the climate by man-made materials and the cycles used to deliver them. Green science could incorporate anything from diminishing waste to discarding waste in the right way. Science assumes a crucial part in deciding the personal satisfaction. The synthetic substances industry and other related ventures supply us a gigantic assortment of fundamental items, from plastics to drugs. Be that as it may, these ventures can possibly genuinely harm our current circumstance. Green science in this way serves to advance the plan and productive utilization of naturally harmless synthetics and substance processes. This load of focuses will be examined in this article.
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