2014
DOI: 10.1371/journal.pone.0107755
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Isolation of Dihydroflavonol 4-Reductase cDNA Clones from Angelonia x angustifolia and Heterologous Expression as GST Fusion Protein in Escherichia coli

Abstract: Blue Angelonia × angustifolia flowers can show spontaneous mutations resulting in white/blue and white flower colourations. In such a white line, a loss of dihydroflavonol 4-reductase (DFR) activity was observed whereas chalcone synthase and flavanone 3-hydroxylase activity remained unchanged. Thus, cloning and characterization of a DFR of Angelonia flowers was carried out for the first time. Two full length DFR cDNA clones, Ang.DFR1 and Ang.DFR2, were obtained from a diploid chimeral white/blue Angelonia × an… Show more

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Cited by 24 publications
(21 citation statements)
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“…Actually, several other plant species, such as Petunia hybrida, Cymbidium hybrida, Angelonia angustifolia, Agapanthus praecox ssp. orientalis (Leighton), have also been found unable to produce pelargonidin derivatives because of the DFR substrate specificities (Mol et al, 1998; Johnson et al, 1999; Gosch et al, 2014; Mori et al, 2014). Therefore, it can be concluded that substrate specificities of FhDFR proteins played crucial role in determination of anthocyanin aglycons in F. hybrdia .…”
Section: Discussionmentioning
confidence: 99%
“…Actually, several other plant species, such as Petunia hybrida, Cymbidium hybrida, Angelonia angustifolia, Agapanthus praecox ssp. orientalis (Leighton), have also been found unable to produce pelargonidin derivatives because of the DFR substrate specificities (Mol et al, 1998; Johnson et al, 1999; Gosch et al, 2014; Mori et al, 2014). Therefore, it can be concluded that substrate specificities of FhDFR proteins played crucial role in determination of anthocyanin aglycons in F. hybrdia .…”
Section: Discussionmentioning
confidence: 99%
“…The genus Angelonia originates in the Neotropical region, which covers the area from central Mexico to the southern tip of South America (BOFF et al, 2014). The emergence of different species is not yet fully understood (PLASCHIL and OLBRICHT, 2008;GOSCH et al, 2014), with report of approximately 50 species (TROPICOS, 2018), and differences related to the shape and color of the flower, ranging between blue, violet, white and pink (GOSCH et al, 2014). The genus can be found in all Brazilian biomes and in the most diverse types of vegetation, including Caatinga, Altitude Field, Clean Field, Rupestrian Field, Cerrado, Dryland Forest, Ombrophilous Forest and Restinga, being registered 18 species in Brazil (REFLORA, 2018).…”
Section: Introductionmentioning
confidence: 99%
“…To achieve this objective a comparison was made in retrieving sequences of two DFR genes from blue flower species and two from white including sequence alignment for homology and protein modeling. Further more the presence of proline rich region along with positions 12 and 26 was reported to be important in determining substrate specificity for DFR of A. angustifolia (Gosch et al, 2014 ). Other positions emphasizing the 26-amino acid region (132–157) were also mentioned in the literature which played a role in utilizing specific dihydroflovonols: DHK, DHQ, and DHM (Johnson et al, 2001 ; Xie et al, 2004 ).…”
Section: Introductionmentioning
confidence: 99%