Isolation of chitosan from Ganoderma lucidum mushroom for biomedical applications

Ospina, Natali Mesa; Álvarez, Sandra Patricia Ospina; Sierra, Diana; Vahos, Diego Fernando Rojas; Ocampo, Paola Andrea Zapata; Orozco, Claudia Patricia Ossa

doi:10.1007/s10856-015-5461-z

Cited by 52 publications

(53 citation statements)

References 28 publications

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“…Similarly, these two sharp peaks at 10° and 20° were recorded for the chitosans from mushroom species F. pinicola and G. lucidum [25,34]. Mesa Ospina et al [34] reported that these peaks are characteristic peaks of the pure chitosan. This indicated that the purity of L. vellereus and P. ribis chitosans are low.…”

Section: Xrdmentioning

confidence: 64%

“…In a study conducted on crustacean chitosan, two sharp peaks at around 10° and 20° were observed [38]. Similarly, these two sharp peaks at 10° and 20° were recorded for the chitosans from mushroom species F. pinicola and G. lucidum [25,34]. Mesa Ospina et al [34] reported that these peaks are characteristic peaks of the pure chitosan.…”

Section: Xrdmentioning

confidence: 80%

“…In the second step, the mass losses of L. vellereus and P. ribis chitosans were 38% and 54%, respectively owing to the degradation of carbohydrate structure. On the contrary, Mesa Ospina et al [34] reported that the mass loss of the mushroom Ganoderma lucidium chitosan was in three steps. The authors explained that the first mass loss was due to evaporation of water in the chitin and the mass losses in the last two steps were due to the degradation of sugar and secondly degradation of organic material.…”

Section: Tgamentioning

confidence: 96%

“…In this study, DTGmax values of chitosans obtained from two mushroom species were found to be 262°C for L. vellereus and 229 °C for P. ribis. The DTGmax values of chitosans were reported as 300°C for silkworm chrysalides [21], 290 °C for potato beetle [36], 265 °C for F. pinicola [25] and 273.2 °C for G. lucidium [34].…”

Section: Tgamentioning

confidence: 99%

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Chitin extraction and chitosan production from cell wall of two mushroom species (Lactarius vellereus and Phyllophora ribis)

Erdoğan

Kaya

Akata

2017

AIP Conference Proceedings

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Abstract.Chitin is an important polysaccharide found as supporting material in the cell wall of mushrooms. In this study, chitin and chitosan were obtained from the cell wall of two different mushroom species using chemical method and physicochemically characterized. The dry weight chitin contents of the mushroom species were determined as 11.4% for Lactarius vellereus and 7.9% for Phyllophora ribis. Chitosan yields of the chitins isolated from L. vellereus and P. ribis were 73.1% and 75.3%, respectively. While, the maximum degradation temperatures of L. vellereus and P. ribis chitins were found to be 354°C and 275°C by thermogravimetric analysis (TGA), the maximum degradation temperature of the chitosans obtained from these chitins were recorded as 262°C and 229°C, respectively. The crystalline index values of L. vellereus and P. ribis chitins were calculated as 64% and 49%, respectively according to the X-ray diffraction analysis (XRD) results. The scanning electron microscopy (SEM) indicated that there were no nanofiber or nanopores on the surface of the chitins and chitosans obtained from these two mushroom species. The results of this study revealed that L. vellereus and P. ribis had higher chitin contents than some other insects and mushroom species recorded in the literature and these species may be used as a potential chitin sources.

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Section: Xrdmentioning

confidence: 64%

Section: Xrdmentioning

confidence: 80%

Section: Tgamentioning

confidence: 96%

Section: Tgamentioning

confidence: 99%

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Chitin extraction and chitosan production from cell wall of two mushroom species (Lactarius vellereus and Phyllophora ribis)

Erdoğan

Kaya

Akata

2017

AIP Conference Proceedings

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“…The most common source of chitin and chitosan is the crustaceous shell; however, mushrooms are an alternative source for isolating these biopolymers because their cellular wall has a high content of chitin, which may be transformed into chitosan through a deacetylation reaction [7].…”

Section: Introductionmentioning

confidence: 99%

Microbial Extraction of Chitin and Chitosan From Pleurotus Spp, Its Characterization and Antimicrobial Activity

Johney¹,

Eagappan

Ragunathan³

2016

Int J Curr Pharm Sci

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Objective: The aim of the present work was to extract the chitin and chitosan from two species of Pleurotus, its characterization and its antimicrobial activity against bacteria and fungi. Methods:The fungi were grown in MGYP medium for 15 d. The chitin and chitosan were extracted using the alkaline method and various biochemical methods and compared with commercial chitin and chitosan. The FTIR and SEM were also performed. Antimicrobial activity was performed using well diffusion method. Results:The maximum yield of mycelia and the chitin and chitosan were obtained on 12 th Conclusion:In the present study the chitin and chitosan were extracted from the Pleurotus spp. (P. florida and P. eous). The yield was maximum in P. florida after 12 day of incubation. P. folrida gave maximum yield (201.3 mg/l of chitin and 65.61 mg/l of chitosan). Fourier Transform Infrared spectroscopy comparing the results obtained with results for commercial chitin and chitosan. Our results showed that chitin and chitosan obtained from Pleurotus spp. had a significant similitude with commercial. SEM image also carried out to study the surface morphology. The fungal chitin and chitosan had more antimicrobial activity against the gram+ve, gram-ve and the various fungus.th Keywords: Chitin, Chitosan, FTIR, SEM, Pleurotus spp., Antibacterial activity day of fermentation. The FTIR and SEM also confirmed the chitin and chitosan. The extracted chitin and chitosan were subjected to antimicrobial activity, and it was found the extracted chitin and chitosan were active against the bacteria and fungi. Our findings suggest that the Pleurotus spp. are the potential candidate to produce eco-friendly chitin and chitosan in the development of drugs, artificial bone and raw material for the food industries in the near future.

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Antioxidant, Cytotoxic and Antimicrobial Activity of Chitosan Preparations Extracted from Ganoderma Lucidum Mushroom

Savin

Crăciunescu

Oancea

et al. 2020

Chemistry & Biodiversity

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Two chitosan extracts were prepared by chemical and enzymatic treatment of Ganoderma lucidum mushroom, as an alternative source to crustacean shells. The molecular weight of the enzymatic extract was lower than that of the chemical one and of shrimp chitosan, as determined by viscosity measurements. Characteristic signals were identified in the 1H‐NMR spectra and high deacetylation degree indicated good physico‐chemical properties for both mushroom chitosan extracts. The scavenging capacity of mushroom chitosan extracts was moderate against the synthetic radicals of 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) and 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH), but higher values were observed for the enzymatic extract, compared to the chemical extract and shrimp chitosan. In vitro cytotoxicity was evaluated in L929 mouse fibroblast cell lines and the results of MTT assay showed good cytocompatibility in the tested range of concentrations. The growth of Gram‐positive bacteria was inhibited more than Gram‐negative bacteria in the presence of mushroom chitosan extracts, in particular by the chemical one, indicating their efficiency as antimicrobial agents. All these results strengthen the evidence of mushroom polysaccharide preparations availability for biomedical applications.

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Isolation of chitosan from Ganoderma lucidum mushroom for biomedical applications

Cited by 52 publications

References 28 publications

Chitin extraction and chitosan production from cell wall of two mushroom species (Lactarius vellereus and Phyllophora ribis)

Chitin extraction and chitosan production from cell wall of two mushroom species (Lactarius vellereus and Phyllophora ribis)

Microbial Extraction of Chitin and Chitosan From Pleurotus Spp, Its Characterization and Antimicrobial Activity

Antioxidant, Cytotoxic and Antimicrobial Activity of Chitosan Preparations Extracted from Ganoderma Lucidum Mushroom

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