Isolation of cDNAs differentially expressed in response to drought stress and characterization of the Ca-LEAL1 gene encoding a new family of atypical LEA-like protein homologue in hot pepper (Capsicum annuum L. cv. Pukang)

Park, Jong A.; Cho, Seok Keun; Kim, Jee Eun; Chung, Hoo Sun; Hong, Jong Pil; Hwang, Baik; Hong, Choo Bong; Kim, Woo Taek

doi:10.1016/s0168-9452(03)00165-1

Cited by 39 publications

(39 citation statements)

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“…cDNAs With the aid of subtractive hybridization and differential display PCR analyses, we previously isolated a broad spectrum of partial cDNA clones from hot pepper seedlings, which were rapidly induced by water deficit [13][14][15]. Among the identified clones, three different cDNA clones, pCa-DI1, pCaDI2, and pCaDI3, encoded partial polypeptides homologous to XTHs in various plant species [14].…”

Section: Isolation and Identification Of The Full-length Caxthmentioning

confidence: 99%

Constitutive expression of abiotic stress‐inducible hot pepper CaXTH3, which encodes a xyloglucan endotransglucosylase/hydrolase homolog, improves drought and salt tolerance in transgenic Arabidopsis plants

et al. 2006

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Xyloglucan endotransglucosylase/hydrolase (XTH) has been recognized as a cell wall-modifying enzyme, participating in the diverse physiological roles. From water-stressed hot pepper plants, we isolated three different cDNA clones (pCaXTH1, pCaXTH2, and pCaXTH3) that encode XTH homologs. RT-PCR analysis showed that three CaXTH mRNAs were concomitantly induced by a broad spectrum of abiotic stresses, including drought, high salinity and cold temperature, and in response to stress hormone ethylene, suggesting their role in the early events in the abiotic-related defense response. Transgenic Arabidopsis plants that constitutively expressed the CaXTH3 gene under the control of the CaMV 35S promoter exhibited abnormal leaf morphology; the transgenic leaves showed variable degrees of twisting and bending along the edges, resulting in a severely wrinkled leaf shape. Microscopic analysis showed that 35S-CaXTH3 leaves had increased numbers of small-sized cells, resulting in disordered, highly populated mesophyll cells in each dorsoventral layer, and appeared to contain a limited amount of starch. In addition, the 35S-CaXTH3 transgenic plants displayed markedly improved tolerance to severe water deficit, and to lesser extent to high salinity in comparison with the wild-type plants. These results indicate that CaXTH3 is functional in heterologous Arabidopsis cells, thereby effectively altering cell growth and also the response to abiotic stresses. Although the physiological function of CaXTHs is not yet clear, there are several possibilities for their involvement in a subset of physiological responses to counteract dehydration and high salinity stresses in transgenic Arabidopsis plants.

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Section: Isolation and Identification Of The Full-length Caxthmentioning

confidence: 99%

Constitutive expression of abiotic stress‐inducible hot pepper CaXTH3, which encodes a xyloglucan endotransglucosylase/hydrolase homolog, improves drought and salt tolerance in transgenic Arabidopsis plants

et al. 2006

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“…Using differential display PCR and subtractive hybridization analysis, we previously identified a broad spectrum of partial cDNA clones from hot pepper seedlings, which were rapidly induced by dehydration (Choi et al, 2002;Park et al, 2003;Hong and Kim, 2005). One of the isolated clones, pCaDRS3 (GenBank accession no.…”

Section: Isolation and Identification Of Full-length Capub1 Cdnamentioning

confidence: 99%

“…Seedlings were grown in a mixture of soil and vermiculite or on Murashige and Skoog medium containing 1% Suc, vitamin B5 (12 mg L 21 ), and 0.8% agar (pH 5.8) in a 25°C growth chamber with a 16-h light/8-h dark photoperiod. Hot pepper plants were subjected to various biotic and abiotic stresses as described by Park et al (2003).…”

Section: Plant Materials and Stress Treatmentsmentioning

confidence: 99%

“…Hot pepper leaf genomic DNA was isolated as described previously (Park et al, 2003). DNA (10 mg/lane) was digested with appropriate enzymes, separated by electrophoresis on 0.7% agarose gel, and blotted onto a nylon membrane filter (Amersham).…”

Section: Dna and Rna Gel-blot Analysesmentioning

confidence: 99%

“…We are interested in elucidating the adaptive response of hot pepper plants against abiotic stresses, such as water deficit. Recently, we have isolated and characterized a broad spectrum of cDNAs from hot pepper plants whose expression is enhanced rapidly in response to drought (Choi et al, 2002;Park et al, 2003;Hong and Kim, 2005). Among the identified cDNAs, pCaDRS3 (GenBank accession no.…”

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Heterologous Expression and Molecular and Cellular Characterization of CaPUB1 Encoding a Hot Pepper U-Box E3 Ubiquitin Ligase Homolog

et al. 2006

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The U-box motif is a conserved domain found in the diverse isoforms of E3 ubiquitin ligase in eukaryotes. From water-stressed hot pepper (Capsicum annuum L. cv Pukang) plants, we isolated C. annuum putative U-box protein 1 (CaPUB1), which encodes a protein containing a single U-box motif in its N-terminal region. In vitro ubiquitination and site-directed mutagenesis assays revealed that CaPUB1 possessed E3 ubiquitin ligase activity and that the U-box motif was indeed essential for its enzyme activity. RNA gel-blot analysis showed that CaPUB1 mRNA was induced rapidly by a broad spectrum of abiotic stresses, including drought, high salinity, cold temperature, and mechanical wounding, but not in response to ethylene, abscisic acid, or a bacterial pathogen, suggesting its role in the early events in the abiotic-related defense response. Because transgenic work was extremely difficult in hot pepper, in this study we overexpressed CaPUB1 in Arabidopsis (Arabidopsis thaliana) to provide cellular information on the function of this gene in the development and plant responses to abiotic stresses. Transgenic Arabidopsis plants that constitutively expressed the CaPUB1 gene under the control of the cauliflower mosaic virus 35S promoter had markedly longer hypocotyls and roots and grew more rapidly than the wild type, leading to an early bolting phenotype. Microscopic analysis showed that 35STCaPUB1 roots had increased numbers of small-sized cells, resulting in disordered, highly populated cell layers in the cortex, endodermis, and stele. In addition, CaPUB1-overexpressing plants displayed increased sensitivity to water stress and mild salinity. These results indicate that CaPUB1 is functional in Arabidopsis cells, thereby effectively altering cell and tissue growth and also the response to abiotic stresses. Comparative proteomic analysis showed that the level of RPN6 protein, a non-ATPase subunit of the 26S proteasome complex, was significantly reduced in 35STCaPUB1 seedlings as compared to the wild type. Pull-down and ubiquitination assays demonstrated that RPN6 interacted physically with CaPUB1 and was ubiquitinated in a CaPUB1-dependent manner in vitro. Although the physiological function of CaPUB1 is not yet clear, there are several possibilities for its involvement in a subset of physiological responses to counteract dehydration and high-salinity stresses in transgenic Arabidopsis seedlings.

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LEA Proteins in Salt Stress Tolerance

Bhardwaj

Sharma

Kanwar

et al. 2013

Salt Stress in Plants

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Isolation of cDNAs differentially expressed in response to drought stress and characterization of the Ca-LEAL1 gene encoding a new family of atypical LEA-like protein homologue in hot pepper (Capsicum annuum L. cv. Pukang)

Cited by 39 publications

References 40 publications

Constitutive expression of abiotic stress‐inducible hot pepper CaXTH3, which encodes a xyloglucan endotransglucosylase/hydrolase homolog, improves drought and salt tolerance in transgenic Arabidopsis plants

Constitutive expression of abiotic stress‐inducible hot pepper CaXTH3, which encodes a xyloglucan endotransglucosylase/hydrolase homolog, improves drought and salt tolerance in transgenic Arabidopsis plants

Heterologous Expression and Molecular and Cellular Characterization of CaPUB1 Encoding a Hot Pepper U-Box E3 Ubiquitin Ligase Homolog

LEA Proteins in Salt Stress Tolerance

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