Constitutive expression of abiotic stress‐inducible hot pepper <i>CaXTH3</i>, which encodes a xyloglucan endotransglucosylase/hydrolase homolog, improves drought and salt tolerance in transgenic <i>Arabidopsis</i> plants

Cho, Seok Keun; Kim, Jee Eun; Park, Jong A.; Eom, Tae Jin; Kim, Woo Taek

doi:10.1016/j.febslet.2006.04.062

Cited by 214 publications

(159 citation statements)

References 48 publications

(79 reference statements)

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“…Accordingly, most XTHs possess either one or both of these activities (Rose et al, 2002). XTH protein activity and transcript levels have also been correlated with various aspects of plant growth and development (Redgwell and Fry, 1993;Bourquin et al, 2002;Hyodo et al, 2003;Matsui et al, 2005;Van Sandt et al, 2007a) in which cellular expansion is required, and this functionality has been confirmed using transgenic approaches (Cho et al, 2006;Osato et al, 2006). Here too, there are instances where this correlation does not hold (Pritchard et al, 1993;Palmer and Davies, 1996).…”

mentioning

confidence: 96%

“…Nevertheless, both expansin and XTH action on the cell wall network probably results in a cell wall that can stretch more easily in response to increased turgor pressure within the cell. Like expansins, XTHs are also regulated in response to environmental changes such as drought and salinity (Cho et al, 2006), wind (Antosiewicz et al, 1997), gravity (Zenko et al, 2004), and pathogen attack (Albert et al, 2004).…”

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confidence: 99%

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The Regulation of Cell Wall Extensibility during Shade Avoidance: A Study Using Two Contrasting Ecotypes of Stellaria longipes

Sasidharan¹,

Chinnappa²,

Voesenek³

et al. 2008

Plant Physiol.

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Shade avoidance in plants involves rapid shoot elongation to grow toward the light. Cell wall-modifying mechanisms are vital regulatory points for control of these elongation responses. Two protein families involved in cell wall modification are expansins and xyloglucan endotransglucosylase/hydrolases. We used an alpine and a prairie ecotype of Stellaria longipes differing in their response to shade to study the regulation of cell wall extensibility in response to low red to far-red ratio (R/FR), an early neighbor detection signal, and dense canopy shade (green shade: low R/FR, blue, and total light intensity). Alpine plants were nonresponsive to low R/FR, while prairie plants elongated rapidly. These responses reflect adaptation to the dense vegetation of the prairie habitat, unlike the alpine plants, which almost never encounter shade. Under green shade, both ecotypes rapidly elongate, showing that alpine plants can react only to a deep shade treatment. Xyloglucan endotransglucosylase/hydrolase activity was strongly regulated by green shade and low blue light conditions but not by low R/FR. Expansin activity, expressed as acid-induced extension, correlated with growth responses to all light changes. Expansin genes cloned from the internodes of the two ecotypes showed differential regulation in response to the light manipulations. This regulation was ecotype and light signal specific and correlated with the growth responses. Our results imply that elongation responses to shade require the regulation of cell wall extensibility via the control of expansin gene expression. Ecotypic differences demonstrate how responses to environmental stimuli are differently regulated to survive a particular habitat.

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mentioning

confidence: 96%

mentioning

confidence: 99%

The Regulation of Cell Wall Extensibility during Shade Avoidance: A Study Using Two Contrasting Ecotypes of Stellaria longipes

Sasidharan¹,

Chinnappa²,

Voesenek³

et al. 2008

Plant Physiol.

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“…Previous reports indicate that hot pepper genes are fully functional in heterologous Arabidopsis cells (Cho et al, 2006a(Cho et al, , 2006bSeo et al, 2008). Numerous independent T4 transgenic lines that exhibited markedly enhanced levels of the Rma1H1 transcript under normal growth conditions were chosen for further analysis ( Figure 4A).…”

Section: Overexpression Of Rma1h1 Confers Drought Tolerance In Arabidmentioning

confidence: 99%

Drought Stress-Induced Rma1H1, a RING Membrane-Anchor E3 Ubiquitin Ligase Homolog, Regulates Aquaporin Levels via Ubiquitination in TransgenicArabidopsisPlants

et al. 2009

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Ubiquitination is involved in a variety of biological processes, but the exact role of ubiquitination in abiotic responses is not clearly understood in higher plants. Here, we investigated Rma1H1, a hot pepper (Capsicum annuum) homolog of a human RING membrane-anchor 1 E3 ubiquitin (Ub) ligase. Bacterially expressed Rma1H1 displayed E3 Ub ligase activity in vitro. Rma1H1 was rapidly induced by various abiotic stresses, including dehydration, and its overexpression in transgenic Arabidopsis thaliana plants conferred strongly enhanced tolerance to drought stress. Colocalization experiments with marker proteins revealed that Rma1H1 resides in the endoplasmic reticulum (ER) membrane. Overexpression of Rma1H1 in Arabidopsis inhibited trafficking of an aquaporin isoform PIP2;1 from the ER to the plasma membrane and reduced PIP2;1 levels in protoplasts and transgenic plants. This Rma1H1-induced reduction of PIP2;1 was inhibited by MG132, an inhibitor of the 26S proteasome. Furthermore, Rma1H1 interacted with PIP2;1 in vitro and ubiquitinated it in vivo. Similar to Rma1H1, Rma1, an Arabidopsis homolog of Rma1H1, localized to the ER, and its overexpression reduced the PIP2;1 protein level and inhibited trafficking of PIP2;1 from the ER to the plasma membrane in protoplasts. In addition, reduced expression of Rma homologs resulted in the increased level of PIP2;1 in protoplasts. We propose that Rma1H1 and Rma1 play a critical role in the downregulation of plasma membrane aquaporin levels by inhibiting aquaporin trafficking to the plasma membrane and subsequent proteasomal degradation as a response to dehydration in transgenic Arabidopsis plants.

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“…Assay of hypocotyl and root growth under salt stress was performed as described by Cho et al (2006). Briefly, wild-type and transgenic Arabidopsis seeds were surface sterilized and plated on 0.8% agar plates (select agar; Life Technologies) containing 0.5 3 Murashige and Skoog salts, 0.5 mM MES, pH 5.7, 1% Suc, and 1 3 vitamin B5.…”

Section: Measurement Of Hypocotyl and Root Lengths Of Wild-type And Tmentioning

confidence: 99%

“…The germination ratio was monitored in the absence or presence of various concentrations of NaCl (10-100 mM) or ABA (0.01-1 mM; Cho et al, 2006).…”

Section: Germination Assaymentioning

confidence: 99%

Heterologous Expression and Molecular and Cellular Characterization of CaPUB1 Encoding a Hot Pepper U-Box E3 Ubiquitin Ligase Homolog

et al. 2006

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The U-box motif is a conserved domain found in the diverse isoforms of E3 ubiquitin ligase in eukaryotes. From water-stressed hot pepper (Capsicum annuum L. cv Pukang) plants, we isolated C. annuum putative U-box protein 1 (CaPUB1), which encodes a protein containing a single U-box motif in its N-terminal region. In vitro ubiquitination and site-directed mutagenesis assays revealed that CaPUB1 possessed E3 ubiquitin ligase activity and that the U-box motif was indeed essential for its enzyme activity. RNA gel-blot analysis showed that CaPUB1 mRNA was induced rapidly by a broad spectrum of abiotic stresses, including drought, high salinity, cold temperature, and mechanical wounding, but not in response to ethylene, abscisic acid, or a bacterial pathogen, suggesting its role in the early events in the abiotic-related defense response. Because transgenic work was extremely difficult in hot pepper, in this study we overexpressed CaPUB1 in Arabidopsis (Arabidopsis thaliana) to provide cellular information on the function of this gene in the development and plant responses to abiotic stresses. Transgenic Arabidopsis plants that constitutively expressed the CaPUB1 gene under the control of the cauliflower mosaic virus 35S promoter had markedly longer hypocotyls and roots and grew more rapidly than the wild type, leading to an early bolting phenotype. Microscopic analysis showed that 35STCaPUB1 roots had increased numbers of small-sized cells, resulting in disordered, highly populated cell layers in the cortex, endodermis, and stele. In addition, CaPUB1-overexpressing plants displayed increased sensitivity to water stress and mild salinity. These results indicate that CaPUB1 is functional in Arabidopsis cells, thereby effectively altering cell and tissue growth and also the response to abiotic stresses. Comparative proteomic analysis showed that the level of RPN6 protein, a non-ATPase subunit of the 26S proteasome complex, was significantly reduced in 35STCaPUB1 seedlings as compared to the wild type. Pull-down and ubiquitination assays demonstrated that RPN6 interacted physically with CaPUB1 and was ubiquitinated in a CaPUB1-dependent manner in vitro. Although the physiological function of CaPUB1 is not yet clear, there are several possibilities for its involvement in a subset of physiological responses to counteract dehydration and high-salinity stresses in transgenic Arabidopsis seedlings.

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Constitutive expression of abiotic stress‐inducible hot pepper CaXTH3, which encodes a xyloglucan endotransglucosylase/hydrolase homolog, improves drought and salt tolerance in transgenic Arabidopsis plants

Cited by 214 publications

References 48 publications

The Regulation of Cell Wall Extensibility during Shade Avoidance: A Study Using Two Contrasting Ecotypes of Stellaria longipes

The Regulation of Cell Wall Extensibility during Shade Avoidance: A Study Using Two Contrasting Ecotypes of Stellaria longipes

Drought Stress-Induced Rma1H1, a RING Membrane-Anchor E3 Ubiquitin Ligase Homolog, Regulates Aquaporin Levels via Ubiquitination in TransgenicArabidopsisPlants

Heterologous Expression and Molecular and Cellular Characterization of CaPUB1 Encoding a Hot Pepper U-Box E3 Ubiquitin Ligase Homolog

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