No abstract

MicroRNAs (miRNAs) are tiny non-coding regulatory molecules that modulate plant's gene expression either by cleaving or repressing their mRNA targets. To unravel the plant actions in response to various environmental factors, identification of stress related miRNAs is essential. For understanding the regulatory behaviour of various abiotic stresses and miRNAs in wheat genotype C-306, we examined expression profile of selected conserved miRNAs viz. miR159, miR164, miR168, miR172, miR393, miR397, miR529 and miR1029 tangled in adapting osmotic, salt and cold stresses. The investigation revealed that two miRNAs (miR168, miR397) were down-regulated and miR172 was up-regulated under all the stress conditions. However, miR164 and miR1029 were up-regulated under cold and osmotic stresses in contrast to salt stress. miR529 responded to cold alone and does not change under osmotic and salt stress. miR393 showed up-regulation under osmotic and salt, and down-regulation under cold stress indicating auxin based differential cold response. Variation in expression level of studied miRNAs in presence of target genes delivers a likely elucidation of miRNAs based abiotic stress regulation. In addition, we reported new stress induced miRNAs Ta-miR855 using computational approach. Results revealed first documentation that miR855 is regulated by salinity stress in wheat. These findings indicate that diverse miRNAs were responsive to osmotic, salt and cold stress and could function in wheat response to abiotic stresses.

Journal articleIFPRI3; ISIEPTDP

To accelerate genetic gains in breeding, physiological trait (PT) characterization of candidate parents can help make more strategic crosses, increasing the probability of accumulating favorable alleles compared to crossing relatively uncharacterized lines. In this study, crosses were designed to complement

The human population is increasing at an alarming rate, whereas heavy metals (HMs) pollution is mounting serious environmental problem, which could lead to serious concern about the future sufficiency of global food production. Some HMs such as Mn, Cu, and Fe, at lower concentration serves as an essential vital component of plant cell as they are crucial in various enzyme catalyzed biochemical reactions. At higher concentration, a vast variety of HMs such as Mn, Cu, Cd, Fe, Hg, Al and As, impose toxic reaction in the plant system which greatly affect the crop yield. Recently, microRNAs (miRNAs) that are small class of non-coding riboregulator have emerged as central regulator of numerous abiotic stresses including HMs. Increasing reports indicate that plants have evolved specialized inbuilt mechanism viz. signal transduction, translocation and sequestration to counteract the toxic response of HMs. Combining computational and wet laboratory approaches have produced sufficient evidences concerning active involvement of miRNAs during HMs toxicity response by regulating various transcription factors and protein coding genes involved in plant growth and development. However, the direct role of miRNA in controlling various signaling molecules, transporters and chelating agents of HM metabolism is poorly understood. This review focuses on the latest progress made in the area of direct involvement of miRNAs in signaling, translocation and sequestration as well as recently added miRNAs in response to different HMs in plants.