Isolation of Campylobacter: what are we missing?

Bolton, F. J.; Hutchinson, D. N.; Parker, Gene

doi:10.1136/jcp.40.6.702-b

Cited by 12 publications

(10 citation statements)

References 4 publications

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“…Campylobacter spp. are fastidious organisms, and culture methods are biased toward the detection of C. jejuni and C. coli (24,25). The relatively high number of FilmArray GI Panel-positive culture-negative C. upsaliensis specimens identified in this study may reflect the fact that C. upsaliensis strains, which are known human pathogens (26,27), are susceptible to the antibiotics used in selective Campylobacter media and are often not recoverable in culture (27)(28)(29).…”

Section: Discussionmentioning

confidence: 82%

Multicenter Evaluation of the BioFire FilmArray Gastrointestinal Panel for Etiologic Diagnosis of Infectious Gastroenteritis

et al. 2015

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fThe appropriate treatment and control of infectious gastroenteritis depend on the ability to rapidly detect the wide range of etiologic agents associated with the disease. Clinical laboratories currently utilize an array of different methodologies to test for bacterial, parasitic, and viral causes of gastroenteritis, a strategy that suffers from poor sensitivity, potentially long turnaround times, and complicated ordering practices and workflows. Additionally, there are limited or no testing methods routinely available for most diarrheagenic Escherichia coli strains, astroviruses, and sapoviruses. This study assessed the performance of the FilmArray Gastrointestinal (GI) Panel for the simultaneous detection of 22 different enteric pathogens directly from stool specimens: Campylobacter spp., Clostridium difficile (toxin A/B), Plesiomonas shigelloides, Salmonella spp., Vibrio spp., Vibrio cholerae, Yersinia enterocolitica, enteroaggregative E. coli, enteropathogenic E. coli, enterotoxigenic E. coli, Shiga-like toxin-producing E. coli (stx 1 and stx 2 ) (including specific detection of E. coli O157), Shigella spp./enteroinvasive E. coli, Cryptosporidium spp., Cyclospora cayetanensis, Entamoeba histolytica, Giardia lamblia, adenovirus F 40/41, astrovirus, norovirus GI/GII, rotavirus A, and sapovirus. Prospectively collected stool specimens (n ‫؍‬ 1,556) were evaluated using the BioFire FilmArray GI Panel and tested with conventional stool culture and molecular methods for comparison. The FilmArray GI Panel sensitivity was 100% for 12/22 targets and >94.5% for an additional 7/22 targets. For the remaining three targets, sensitivity could not be calculated due to the low prevalences in this study. The FilmArray GI Panel specificity was >97.1% for all panel targets. The FilmArray GI Panel provides a comprehensive, rapid, and streamlined alternative to conventional methods for the etiologic diagnosis of infectious gastroenteritis in the laboratory setting. The potential advantages include improved performance parameters, a more extensive menu of pathogens, and a turnaround time of as short as 1 h. Infectious gastroenteritis (IGE) is a leading cause of global morbidity and mortality. It is estimated that IGE contributes to the death of 2,195 children each day (1). IGE also contributes to serious morbidities, such as malnutrition, stunting, and impaired cognitive function (2, 3). Diarrheal disease disproportionately affects developing nations, but IGE remains a significant problem in industrialized countries as well. For example, it is estimated that each year, approximately 178.8 million cases of gastrointestinal illness occur in the United States, resulting in 474,000 hospitalizations and 5,000 deaths (4). Although the etiologic agents responsible for about 80% of these illnesses are unidentified or otherwise unspecified (4), norovirus and Salmonella spp. are currently the most commonly identified pathogens associated with food-borne disease in the United States and account for 5.5 and 1.0 million cases each year, resp...

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Section: Discussionmentioning

confidence: 82%

Multicenter Evaluation of the BioFire FilmArray Gastrointestinal Panel for Etiologic Diagnosis of Infectious Gastroenteritis

et al. 2015

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“…This medium, however, isolated most of the "Campylobaeter cinaedi" and "Campylobacter fennelliae" strains (Table 4). In the past these strains have been isolated from homosexuals (3-6) and dogs (16), but in the present survey they were isolated from diarrhoeal stool samples submitted routinely to the laboratory. The pathogenicity of these organisms is still not clear, but they have been isolated from blood cultures in the USA (5, 6) and the UK (unpublished data).…”

Section: Discussionmentioning

confidence: 98%

Reassessment of selective agars and filtration techniques for isolation ofCampylobacter species from faeces

Bolton¹,

Hutchinson²,

Parker³

1988

Eur. J. Clin. Microbiol. Infect. Dis.

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Four different studies were conducted in order to re-evaluate conventional methods and assess the efficacy of new selective agars and a filtration method for the isolation of campylobacters. Skirrow's medium, Preston agar, modified CCD agar and Fennell's medium, incubated microaerobically at 37 degrees C for 48 h, gave similar Campylobacter isolation rates from 225 faecal samples, but the latter two media were more selective. Evaluation of modified CCD agar demonstrated that campylobacters could be isolated from that medium more successfully after incubation at 37 degrees C (173/177 positive samples) than at 42 degrees C (152/177 positive samples). In a larger study 1286 faecal specimens were cultured using modified CCD agar, Fennell's medium and a 0.45 micron membrane filtration technique, all incubated at 37 degrees C. Campylobacters were isolated from 89% (178), 86% (171) and 60% (130) of 199 positive samples respectively. Modified CCD agar was most successful in isolation of the majority of campylobacters, but Fennell's medium was essential for recovery of "Campylobacter cinaedi" and "Campylobacter fennelliae", whereas the 0.45 micron membrane technique was the only method to isolate all of the catalase-negative campylobacter strains. Further evaluation of the 0.45 micron and 0.65 micron pore size membranes showed that more strains of Campylobacter jejuni and Campylobacter coli were isolated using the larger pore size membranes.

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“…Therefore the pathogenic significance of C. jejrrni may be a reflection of the high specificit! of selective isolation protocols toward this species, under-estimating non-jqjunr genus members (Bolton et al 1987b). …”

Section: The Genus Campylobactermentioning

confidence: 99%

Campylobacter epidemiology: an aquatic perspective

Thomas¹,

Gibson²,

Hill³

et al. 1998

Journal of Applied Microbiology

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SUMMARY Members of the genus Campylobacter have established themselves as the most common human gastro‐enteric pathogens throughout much of the developed world. The ubiquitous distribution of Campylobacter spp. in animal reservoirs and food products derived thereof make such vehicles primary risk factors in contracting campylobacteriosis. The contamination rates, identification of common pathogenic serotypes and extended survival of Campylobacter in surface waters illustrates the potential, but yet to be quantified, campylobacteriosis risk associated with untreated water. The existence and potential pathogenicity of viable but nonculturable forms of Campylobacter remains a contentious subject. Furthermore, the role of such forms in the epidemiology of Campylobacter related disease and their involvement in the large number of waterborne gastroenteritis outbreaks from which a disease agent cannot be isolated remains to be fully clarified. This article presents a survey of current perspectives with regard to the survival and epidemiology of Campylobacter spp. in natural water systems.

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Isolation of Campylobacter: what are we missing?

Cited by 12 publications

References 4 publications

Multicenter Evaluation of the BioFire FilmArray Gastrointestinal Panel for Etiologic Diagnosis of Infectious Gastroenteritis

Multicenter Evaluation of the BioFire FilmArray Gastrointestinal Panel for Etiologic Diagnosis of Infectious Gastroenteritis

Reassessment of selective agars and filtration techniques for isolation ofCampylobacter species from faeces

Campylobacter epidemiology: an aquatic perspective

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