Infectious bovine rhinotracheitis (IBR) is worldwide disease affecting both domesticated and wild ruminants However, it has a significant economic impact on animal industry. Bovine herpes virus 1 (BoHV-1) can infect different organs of an animal. The current work aims to investigate the prevalence of BoHV-l in cattle of Mosul city, Iraq. In this study, 184 animals 6 months – 2 years old, were sampled between October 2020 and March 2022. Plasma and nasal swabs were tested using of Sandwich ELISA test for the detection of BoHV-l antigens. Results indicated that the infection rate of BoHV-l in cows was 12/184 (6.5%). Results show that, there was no significant difference indicated among prevalence of the diseases concerning BoHV-1 in the respiratory, ocular, and reproductive forms, the sample type, whether plasma and nasal swabs, between native and imported breed (P≤0.05). Furthermore, a significant difference in the prevalence of BoHV-1 was detected in males more than in females 12/144 (8.3%), (P≤0.05). In addition, there was a significant difference in the prevalence of BoHV-1 10/100 (10%) at 6-8 months of age (P≤0.05).