Isolation of Antigenic Fractions from Bordetella pertussis by means of Lysozyme

Pusztai, Z; Joó, I.; Eckhardt, E.R.

doi:10.1038/196296a0

Cited by 4 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Soluble preparations of HSF have been obtained from culture supernatant fluids (74, 118,119,172), from sonically disrupted cells (51,119,239), from cells dissolved with sodium deoxycholate (17, 42,237), from cells treated with lysozyme (15,16,139,211), by extraction of cells with a mixture of thiourea-urea and formamide (76,118), by autolysis (119), and from acetone-dried cells extracted with alkaline saline (160,161). These soluble preparations have supplied materials for further purification of HSF.…”

Section: Nature Of the Histamine-sensitizing Factor (Hsf) From Bordetmentioning

confidence: 99%

“…The activity of HSF is destroyed by heat (80 C for 0.5 hr) (8,90,97,119,162,172), by Formalin (88, 91, 136, 162), and by some proteolytic enzymes such as trypsin [Sutherland (227) did not obtain destruction by trypsin], Pronase, and a protease from Bacillus subtilis (82,107,138,195). Deoxyribonuclease, ribonuclease (237), lysozyme (15,16,139,211), lipase, cellulase, and amylase (238) do not destroy it.…”

Section: General Properties Of Hsf From B Pert Ussismentioning

confidence: 99%

See 1 more Smart Citation

Histamine-sensitizing factors from microbial agents, with special reference to Bordetella pertussis.

Muñoz¹,

Bergman²

1968

Bacteriological Reviews

View full text Add to dashboard Cite

a Toxicity = LD5so of histamine given intraperitoneally, expressed as milligrams of histamine base per kilogram of body weight. b J. Munoz, unpublished data. toxin. This reaction was most probably an anaphylactic shock, although it was not recognized as such by Ospeck and Roberts. Parfentjev et al. (186-188) observed a similar phenomenon when they found that mice pretreated with pertussis vaccine died after receiving a denatured nucleoprotein isolated from B. pertussis. This sensitivity was considered to have an immunological basis, but it was not clearly recognized as being associated with anaphylactic shock until Malkiel and Hargis described their observations on the increased susceptibility of B. pertussis-treated mice and rats to actively induced anaphylaxis (121-124). These observations have been confirmed and extended by various workers employing a variety of antigens (100, 154, 205, 223). Furthermore, B. pertussis-treated mice were also seen to become more susceptible to passively induced anaphylaxis (

show abstract