Histamine-sensitizing factors from microbial agents, with special reference to Bordetella pertussis.

Muñoz, J.; Bergman, R. K.

doi:10.1128/mmbr.32.2.103-126.1968

Cited by 45 publications

(13 citation statements)

References 89 publications

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“…It was revealed that purified LPF consisted solely of uniform filamentous molecules of about 2 by 40 nm. (16), including leucocytosis. Recently, Morse et al (11,12,14,15) studied the mechanisms involved in development of leucocytosis in mice injected with pertussis cells.…”

Section: Resultsmentioning

confidence: 99%

Leucocytosis-Promoting Factor of Bordetella pertussis I. Purification and Characterization

Sato

Arai

1972

Infect Immun

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The leucocytosis-promoting factor was purified from the supernatant fluid of spent cultures of Bordetella pertussis on solid medium. After precipitation at 67% saturation of ammonium sulfate, the leucocytosis-promoting factor was extracted with a 1.0 M NaCl solution. Purification was accomplished by starch block electrophoresis and sucrose density gradient centrifugation. The purified preparation contained a high leucocytosis-promoting activity, and as small an amount as 0.04 ,ug of protein induced leucocytosis in mice. About 520-fold purification was attained, with a rerecovery of about 25 % on an activity basis. The leucocytosis-promoting factor was composed solely of filamentous molecules of about 2 by 40 nm in size, with a sedimentation coefficient of approximately 5.5S and a molecular weight of 108,000. It was insoluble in water but partially soluble in 1.0 M NaCl solution, and consisted mainly of protein, with some carbohydrate, lipid, and phosphorus.on August 8, 2020 by guest http://iai.asm.org/ Downloaded from

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Section: Resultsmentioning

confidence: 99%

Leucocytosis-Promoting Factor of Bordetella pertussis I. Purification and Characterization

Sato

Arai

1972

Infect Immun

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“…The injection of killed PO in humans and animals produces numerous biological phenomena. In mice, leukocytosis, splenomegaly, increase in sensitivity to agents such as histamine or serotonin, induction of nonspecific resistance to infection, potentiation of tumor defense mechanisms, and adjuvant activity are the most prominent features (8,22). Recent studies (H. Hof et al, submitted for publication) have shown that the time interval between the PO injection and the infection with L. monocytogenes is of crucial significance for increased resistance to L. monocytogenes.…”

Section: Infect Immunmentioning

confidence: 99%

Reversion of dextran sulfate-induced loss of antibacterial resistance by Bordetella pertussis

Finger¹,

B²,

Wirsing³

et al. 1978

Infect Immun

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Parenteral injection of dextran sulfate 500 (DS 500; 50 mg/kg of body weight) into mice caused a complete loss of resistance to a sublethal (2 x 103 to 5 x 103) infection with Listeria monocytogenes. Such loss could be prevented by pretreatment of animals with 3 x 109 heat-killed Bordetella pertussis organisms (PO) 5 to 30 days before the administration of DS 500. The increased phagocytic capacity induced by PO was only exhausted when a fourfold dose of DS 500, effecting complete loss of antibacterial resistance (50 mg/kg of body weight), was administered. Listeriosis in mice treated with DS 500 is characterized by rapid-progressive necro-purulent inflammation of liver and spleen, lack of mononuclear phagocyte response, and 100% lethality within 72 h after infection. In contrast, the time course, extent, and morphological characteristics of listeriosis in animals pretreated with PO before the DS 500 application were not significantly different from those of nonpretreated controls. Evidence is presented that the protective effect of PO is due to activation of the mononuclear phagocyte system, which without such treatment is blocked by the DS 500 administration. The data presented indicate that the protective effect of PO is due only in part to the endotoxic moiety of these bacteria. Differences in the course and morphology of listeriosis in animals with dysfunction of the mononuclear phagocyte system and in animals with deficiency of the cellular immune system are discussed.

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“…After injection ofB. pertussis, mice and rats which are normally resistant to the lethal effects of histamine become highly sensitive (4)(5)(6)(7). Histamine-sensitizing factor (HSF) activity was assayed in pertussis-treated mice by injecting the equivalent of 1 mg of histamine-free base, as histamine dihydrochloride (sehwarz/Mann Div., Becton, Dickinson & Co., Orangeburg, N.…”

Section: Biological Assaysmentioning

confidence: 99%

Isolation and properties of the leukocytosis- and lymphocytosis-promoting factor of Bordetella pertussis.

Morse¹,

Morse²

1976

The Journal of Experimental Medicine

166

105

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In a previous report on the effects of the leukocytosis-and lymphocytosispromoting factor (LPF) 1 ofBordetella pertussis on cyclic adenosine 3':5'-monophosphate (cAMP) metabolism, a method for isolation of highly active LPF was presented briefly (1). In the present communication, the results of further studies on the isolation and chemical, immunochemical, and biological properties of LPF are described. It has been found that a single moiety is responsible for the induction in mice of leukocytosis with both lymphocytosis and granulocytosis, histamine sensitization, and hypoglycemia and unresponsiveness to the hyperglycemic effects of epinephrine. In confirmation of our preliminary report (2), LPF has been found to be clearly distinct from the hemagglutinating pili of B. pertussis, the properties of which are also described. Materials and MethodsB. pertussis Cultures. Strain NIH 114 (3779B), which has been utilized in continuing studies in this laboratory was also employed in the present investigations. Lyophilizod samples, obtained through the courtesy of Dr. Charles Manclark, Bureau of Biologics, Bethesda, Md., were reconstituted in sterile distilled H~O and plated on Bordet-Gengou medium (Clinical Sciences Inc., Whippany, N. J.). The 2-day growth at 36°C on one Petri dish was harvested in 10 ml of 2% casamino acids (technical grade; Difco Laboratories, Detroit, Mich.), and 1 ml of the suspension was inoculated into 100 ml of liquid medium in i liter Blake bottles. After 2 days of incubation at 36°C, 10 ml of the seed culture (approximately 5 × 10 x° organJma) was inoculated into 1 liter of liquid medium in Povitsky bottles which were then incubated at 35-36°C. In order to provide maximum surface area for aeration, all bottles were placed in the horizontal position during incubation. In the case of the 100 ml cultures, the depth of the medium was 1.0 cm and the surface area 218 cm2; the values for the 1-1iter cultures were 2.5 cm and 580 cm 2, respectively.The liquid medium was that previously described (3) except for the omission of an anion exchange resin which was found to be unnecessary for either optimal growth, maintenance of the Phase I state, or maximal production of LPF. Culture density was assessed with the National Institutes of Health opacity standard, and the criteria utilized to determine whether the organisms were Phase I included: a homogenous population of small, gram-negative coccobacilli and

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Histamine-sensitizing factors from microbial agents, with special reference to Bordetella pertussis.

Cited by 45 publications

References 89 publications

Leucocytosis-Promoting Factor of Bordetella pertussis I. Purification and Characterization

Leucocytosis-Promoting Factor of Bordetella pertussis I. Purification and Characterization

Reversion of dextran sulfate-induced loss of antibacterial resistance by Bordetella pertussis

Isolation and properties of the leukocytosis- and lymphocytosis-promoting factor of Bordetella pertussis.

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