Isolation of Adipose-Derived Stem/Stromal Cells from Cryopreserved Fat Tissue and Transplantation into Rats with Spinal Cord Injury

Ohta, Yuki; Takenaga, Mitsuko; Hamaguchi, Akemi; Ootaki, Masanori; Takeba, Yuko; Kobayashi, Tsukasa; Watanabe, Minoru; Iiri, Taroh; Matsumoto, Naoki

doi:10.3390/ijms19071963

Cited by 23 publications

(32 citation statements)

References 25 publications

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“…Rat subcutaneous adipose tissue was obtained through surgery as previously described with modifications [27]. Briefly, rats were anesthetized by 2% sodium pentobarbital, and the skin was sterilized by 70% ethanol and opened by a pair of scissors.…”

Section: Preparation Of Rascsmentioning

confidence: 99%

Transplantation Site Affects the Outcomes of Adipose-Derived Stem Cell-Based Therapy for Retinal Degeneration

Wei

et al. 2020

Stem Cells International

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Adipose-derived stem cells (ASCs) have shown a strong protective effect on retinal degenerative diseases (RDD) after being transplanted into the subretinal space in an animal model. Recently, several clinical trials have been conducted to treat RDD with intravitreal transplantation of stem cells, including ASCs. However, the outcomes of the clinical trials were not satisfactory. To investigate if the transplantation site alters the outcome of stem cell-based therapy for RDD, we isolated rat ASCs (rASCs) and labeled them with green fluorescent protein. Autologous rASCs were grafted into the vitreous chamber or subretinal space in a rat RDD model induced by sodium iodate (SI). The electric response was recorded by ERG. The anatomic structure of the retina was observed in cryosections of rat eyes at posttransplantation weeks 1, 2, and 4. Neural retina apoptosis and epiretinal membrane-(ERM-) like structure formation were investigated by immunostaining. The intravitreal transplantation of rASCs resulted in an extinguished electric response, although the rosette formation and apoptosis of neural retina were reduced. However, the rASCs that grafted in the subretinal space protected the retina from the damage caused by SI, including a partial recovering of the electric response and a reduction in rosette formation. Intravitreally grafted rASCs formed a membrane, resulting in retina folding at the injection site. Müller cells, retinal pigment epithelial cells, and microglial cells migrated from the retina to the rASC-formed membrane and subsequently formed an ERM-like structure. Furthermore, vitreous fluid promoted rASC migration, and rASC-conditioned medium enhanced Müller cell migration as indicated by in vitro studies. These data suggested that the vitreous chamber is not a good transplantation site for ASC-based therapy for RDD and that a deliberate decision should be made before transplantation of stem cells into the vitreous chamber to treat RDD in clinical trials.

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Section: Preparation Of Rascsmentioning

confidence: 99%

Transplantation Site Affects the Outcomes of Adipose-Derived Stem Cell-Based Therapy for Retinal Degeneration

Wei

et al. 2020

Stem Cells International

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“…As adherent cells reached 80% confluence (passage 1: P1), hADSCs were detached with 0.25% trypsin/1mM ethylenediamine tetraacetic acid (EDTA) (Sigma) and plated in flasks at a density of 1,5×10 4 cells/75 cm 2 (passage 2: P2). The cellular density was determined by manually counting the number of cells at each division 36 . Identified as cell 1 (C1). Patient-derived hAT-MSCs: cells were obtained from the subcutaneous fat of a 30-year-old female, submitted to abdominal liposuction at the Hospital de Clínicas in Porto Alegre, Brazil.…”

Section: Methodsmentioning

confidence: 99%

“…The cellular density was determined by manually counting the number of cells at each division 36 Both cells C1 and C2 were expanded under the same conditions and used only from 4 th to 8 th passage 26,34,37 . An aliquot of 1x10 4 hAT-MSCs was placed on a slide and analyzed by immunofluorescence.…”

Section: Sources and Culture Of Hat-mscsmentioning

confidence: 99%

Extracellular vesicles from human adipose stem cells are neuroprotective after stroke in rats

Rohden

et al. 2020

Preprint

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Ischemic stroke is a prominent cause of death and disability, demanding innovative therapeutic strategies. Accordingly, extracellular vesicles (EVs) released from mesenchymal stem cells are promising tools for stroke treatment. In this study, we evaluated the potential neuroprotective properties of EVs released from human adipose tissue stem cells (hAT-MSC), which were obtained from a healthy individual submitted to liposuction. A single intranasal EVs administration was performed 24 h after the ischemic stroke in rats. The EVs brain penetration and the tropism to brain zone of ischemia was observed 18 h after administration. Thus, we measured EVs neuroprotection against the ischemic stroke-induced impairment on long-term motor and behavioral performance. Indeed, one single intranasal EVs administration reversed the stroke damages on: i) front paws symmetry; ii) working memory, short- and long-term memory; iii) anxiety-like behavior. These findings highlight hAT-MSC-derived EVs as a promising therapeutic strategy in stroke.

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“…This procedure allows accomplishing an archive of stem cells avoiding donor variables such as health status and age at the time of collection. With the same aim, the research attempt to develop safe and efficient long-term biobanks for human fat to be use as source for ASCs [ 100 ].…”

Section: Adipose Stem Cellsmentioning

confidence: 99%

Adipose Stem Cell Translational Applications: From Bench-to-Bedside

Argentati

Morena

Bazzucchi

et al. 2018

IJMS

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During the last five years, there has been a significantly increasing interest in adult adipose stem cells (ASCs) as a suitable tool for translational medicine applications. The abundant and renewable source of ASCs and the relatively simple procedure for cell isolation are only some of the reasons for this success. Here, we document the advances in the biology and in the innovative biotechnological applications of ASCs. We discuss how the multipotential property boosts ASCs toward mesenchymal and non-mesenchymal differentiation cell lineages and how their character is maintained even if they are combined with gene delivery systems and/or biomaterials, both in vitro and in vivo.

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Isolation of Adipose-Derived Stem/Stromal Cells from Cryopreserved Fat Tissue and Transplantation into Rats with Spinal Cord Injury

Cited by 23 publications

References 25 publications

Transplantation Site Affects the Outcomes of Adipose-Derived Stem Cell-Based Therapy for Retinal Degeneration

Transplantation Site Affects the Outcomes of Adipose-Derived Stem Cell-Based Therapy for Retinal Degeneration

Extracellular vesicles from human adipose stem cells are neuroprotective after stroke in rats

Adipose Stem Cell Translational Applications: From Bench-to-Bedside

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