Extracellular vesicles from human adipose stem cells are neuroprotective after stroke in rats

Abstract

Ischemic stroke is a prominent cause of death and disability, demanding innovative therapeutic strategies. Accordingly, extracellular vesicles (EVs) released from mesenchymal stem cells are promising tools for stroke treatment. In this study, we evaluated the potential neuroprotective properties of EVs released from human adipose tissue stem cells (hAT-MSC), which were obtained from a healthy individual submitted to liposuction. A single intranasal EVs administration was performed 24 h after the ischemic stroke in rats. The EVs brain penetration and the tropism to brain zone of ischemia was observed 18 h after administration. Thus, we measured EVs neuroprotection against the ischemic stroke-induced impairment on long-term motor and behavioral performance. Indeed, one single intranasal EVs administration reversed the stroke damages on: i) front paws symmetry; ii) working memory, short- and long-term memory; iii) anxiety-like behavior. These findings highlight hAT-MSC-derived EVs as a promising therapeutic strategy in stroke.

Exosomes as a therapeutic tool to promote neurorestoration and cognitive function in neurological conditions: Achieve two ends with a single effort

“…EVs derived from adipose tissue stem cells (hAT‐MSC) of the healthy individual, referred to as liposuction, reverse the destructive effect of cerebral stroke on motor function, working memory, short‐term memory, long‐term memory, and anxiety‐like behaviors. EVs‐delivered animals spent more time with the novel object in the NORT, had more spontaneous alternation in the Y‐Maze, and spent more time in the open arm of the EPM 126 . Furthermore, Zeb2/Axin2‐supplemented exosomes, harvested from BMSCs transfected plasmid, improve the endogenous neurogenesis and neurobehavioral recovery of ischemic animals, especially in spatial memory manner with decreasing the latency to find the platform in the probe trial of MWM test 26 …”

“…EVs‐delivered animals spent more time with the novel object in the NORT, had more spontaneous alternation in the Y‐Maze, and spent more time in the open arm of the EPM. 126 Furthermore, Zeb2/Axin2‐supplemented exosomes, harvested from BMSCs transfected plasmid, improve the endogenous neurogenesis and neurobehavioral recovery of ischemic animals, especially in spatial memory manner with decreasing the latency to find the platform in the probe trial of MWM test. 26 …”