Isolation, molecular identification and characterization of Aeromonas hydrophila from infected air-breathing catfish Magur (Clarias batrachus) cultured in Mymensingh, Bangladesh

Abstract

The study was carried out from November, 2014 to February, 2015 with the objective of isolating Aeromonas hydrophila an important fish pathogen from infected air-breathing catfish Magur (Clarias batrachus) in Mymensingh district. Quantitative study of the isolated bacteria from infected C. batrachus was found variation of number in different organs. Total bacterial load was found to be 1.16 x 105 to 3.15 x 106 cfu/g in lesions, 2.14 x 108 to 4.17 x 109 cfu/g in liver, 1.90 x 107 to 5.12 x 108 cfu/g in spleen and 2.32 x 106 to 5.24 x 108 cfu/g in kidney of infected C. batrachus in Mymensingh district. The isolates were found to produce acid from arabinose, whereas acid and gas from different sugar media such as maltose, sucrose, and dextrose. Morover, they were capable to ferment glucose but resistant to vibriostatic agent 0129 test. Further identification of A. hydrophila was accomplished using PCR. The PCR products of desired 760 bp were obtained for A. hydrophila. The isolated A. hydrophila were 96% sensitive to Enrofloxacin followed by 88% to Ciprofloxacin and 76% to Levofloxacin. On the other way, 100% were resistant to the Ampicillin followed by 96% to Penicillin and 92% to Novobiocin. So far, this is the first molecular identification of A. hydrophila from farmed C. batrachus in Bangladesh. The present study will provide future research scopes on identification of pathogenicity island in chromosome and serotyping of all A. hydrophila isolates.
 Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 17-24

Water as a Source of Indoor Air Contamination with Potentially Pathogenic Aeromonas hydrophila in Aquaculture

“…In a study of A. hydrophila isolated from fish ponds in semi-intensive aquaculture in the late stage of the carp fattening cycle, 95%, 65%, and 15% of the isolates were resistant to trimethoprim, tetracycline, and chloramphenicol, respectively. Monir et al [44] isolated A. hydrophila strains from air-breathing magur catfish (Clarias batrachus) in aquaculture and found that 100% of the isolates were resistant to ampicillin, 96% were resistant to penicillin, and 92% were resistant to novobiocin. Aeromonas hydrophila strains isolated from intensive aquaculture in the Southeast Region of Brazil were moderately resistant to amoxicillin, ampicillin, lincomycin, novobiocin, oxacillin, penicillin, and tetracycline [45].…”

Aeromonas hydrophila infection induces Toll-like receptor 2 (tlr2) and associated downstream signaling in Indian catfish, Clarias magur (Hamilton, 1822)

“…The freshwater air-breathing Indian catfish, C. magur (genetically distinct from C. batrachus ) ( Devassy et al, 2015 ) is a promising candidate species for freshwater aquaculture in India next to carp ( Sahoo et al, 2016 ). Magur is prone to bacterial and fungal infection in all phase of farming due to scale-less body and many Gram-negative bacterial pathogens have been reported from magur includes, A. hydrophila , A. veronii, Flavobacterium spp, Edwardsiella tarda, E. ictaluri, Pseudomonas spp ( Monir et al, 2017 ; Sharma et al, 2017 ; Sharma et al, 2018 ). There are no reports on Gram-positive bacterium causing disease in Indian magur.…”

Pharmacokinetics and physio‐metabolic response of single and multiple dose of fenbendazole inLabeo rohita(Hamilton, 1822) fingerlings

“…Parasitic infection interferes the metabolism, and secretary functions of the alimentary canal, which affects the nervous system and causes the nutritional deficiency in the fish (Markov, 1961). Among IMC, L. rohita is found to be more susceptible to parasitic diseases than C. catla and C. mrigala (Monir et al., 2015).…”

“…High stocking density in intensive systems poses the stress on fish to facilitate the opportunistic pathogens to cause diseases. In intensive aquaculture system, 15%–20% diseases are reported due to parasite infection (Monir et al., 2015). Parasitic infection interferes the metabolism, and secretary functions of the alimentary canal, which affects the nervous system and causes the nutritional deficiency in the fish (Markov, 1961).…”

“…aquaculture system, 15%-20% diseases are reported due to parasite infection (Monir et al, 2015). Parasitic infection interferes the metabolism, and secretary functions of the alimentary canal, which affects the nervous system and causes the nutritional deficiency in the fish (Markov, 1961).…”