Isolation, Molecular Characterization and Antimicrobial Resistance Patterns of Four Different Vibrio Species Isolated from Fresh Water Fishes

Suresh, Y; Subhashini, N; Kiranmayi, Ch. Bindu; Srinivas, K.; Ram, Ved; Gottapu, Chaitanya; Vimala, B.; Rao, T. Srinivasa

doi:10.20546/ijcmas.2018.707.359

Cited by 9 publications

(6 citation statements)

References 26 publications

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“…Species specific genes used for confirmation and differentiation among Vibrio species. According to Tarr et al, 2007, V. cholera was detected by sodB gene that show light band at 248 bp and V. parahaemolyticus was identified by toxR gene that produced light band at 368 bp as mentioned by Kim et al, 1999. In our study, the occurrence of Vibrio species was found 93.3% compared to 98.67%, 82.87% and 27.7% as reported by Noorlis et al, 2011, Suresh et al, 2018and El-Hady et al, 2015 respectively, the occurrence of V. cholera was found 57.1% this result is higher than the result obtained by Asran et al, 2020 andArunagiri et al, 2016 who recorded it with percentage 3.45% and 22.4% respectively and the occurrence of V. parahaemolyticus was found `42.9% compared to 75.9%, 50% and 17.24% reported by Anjay et al, 2014, Asran et al, 2020and Arunagiri et al, 2016 This difference in prevalence percentages may be related to difference in area, fish species, change in the fish immune system and time and methods of sampling and water quality characters (Abdelaziz et al, 2017). The detection of virulence genes in each species is so important to detect the ability of the bacteria to produce the disease.…”

Section: Discussionsupporting

confidence: 67%

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Microbiological and molecular characterization of Vibrio cholera and Vibrio parahaemolyticus isolated from Tilapia fish (Oreochromis niloticus

Ali

Al-Azeem

Younis

2023

SVU-International Journal of Veterinary Sciences

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Vibrio species are zoonotic pathogens that can affect humans by different routes such as ingestion or contact causing gastrointestinal diseases and wound infection. This study aimed to detect V. cholera and V. parahaemolyticus in Nile Tilapia fish collected from Aswan Governorate, Egypt. A total of 52 muscle samples were collected from Tilapia fish and were subjected to microbiological and molecular characterization. Alkaline peptone water media was used for the enrichment of the samples then followed by inoculation onto thiosulfate citrate bile salt sucrose (TCBS) agar media for the isolation of Vibrio species, biochemical tests were performed to identify V. cholera and V. parahaemolyticus, and then they were confirmed by Vibrio genus-specific gene and virulence genes by PCR . Out of 52 fish muscle samples (Nile Tilapia) 32 appeared as yellow colonies, 2 samples showed green colonies and 18 samples showed mixed yellow and green on TCBS agar. Only 40 samples show biochemically positive for Vibrio species. 15 random samples were amplified to 16srRNA gene by PCR technique for more accurate identification resulting in 14 isolates being positive to 16srRNA, 8 isolates positive for sodB gene-specific of V. cholera, and 6 isolates positive for toxR gene-specific of V. parahaemolyticus. Out of 8 V. cholera isolates were found high resistance rate to amoxiclav (87.5%), gentamicin (75%), chloramphenicol (50 %) and ampicillin (50 %). Conversely, (50 %) sensitive to tetracycline, ciprofloxacin and, trimethoprimsulphamethoxazole, and the average MRA of V. cholera was 0.48. On the other hand, all 6 V. parahaemolyticus isolates were resistant to amoxiclav (100%), tetracycline (100%), gentamicin (100%) and chloramphenicol (100%) However, all isolates sensitive to trimethoprimsulphamethazole (100%) but half of them sensitive to ciprofloxacin (50%) and ampicillin (50%), and the average MRA of V. parahaemolyticus was 0.59. In conclusion, this study showed that V. cholera is the most dominant pathogenic one then followed by V. parahaemolyticus in Nile tilapia fish resulting in economic losses and causing public health problems.

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Section: Discussionsupporting

confidence: 67%

“…PCR products were subjected to gel electrophoresis with 1.5% agarose gel. Then the gel was submerged in ethidium bromide as fluorescent dye and visualized using Gel Documentation unit (BIORAD, USA) (Suresh et al, 2018).…”

Section: Undermentioning

confidence: 99%

Microbiological and molecular characterization of Vibrio cholera and Vibrio parahaemolyticus isolated from Tilapia fish (Oreochromis niloticus

Ali

Al-Azeem

Younis

2023

SVU-International Journal of Veterinary Sciences

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“…DNA was separated by boiling and chilling method (Suresh et al, ). Molecular confirmation of presumptive colonies was done by targeting the genus and species specific genes (Brisse & Verhoef, ; Chander, Ramakrishnan, Jindal, Hanson, & Goyal, ; Kovtunovych et al, ) (Table and Figure ).…”

Section: Methodsmentioning

confidence: 99%

Occurrence and genetic diversity of ESBL producing Klebsiella species isolated from livestock and livestock products

Bobbadi¹,

Chinnam²,

Nelapati³

et al. 2019

Journal of Food Safety

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Klebsiella species have been at the center of attention over the recent years due to its role in the evolution of antimicrobial resistance and is not only associated with nosocomial but also with food related infections worldwide. In this study, out of 336 samples of animal intestinal and foods of animal origin screened, 99 samples were found to harbor Klebsiella spp. Out of 99 isolates, 89 were Klebsiella pneumoniae and 10 were found to be Klebsiella oxytoca by mPCR. The isolates were subjected to antibiotic sensitivity test including ESBL detection by genotypic (PCR) and phenotypic (disc diffusion) methods. β-Lactamase genes were identified in 32 isolates of K. pneumoniae and an isolate of K. oxytoca, blaSHV being the predominant gene detected (31) followed by blaTEM, blaCTX-M1, and blaCTX-M9 (one each), whereas single K. oxytoca isolate harbored blaCTX-M2 gene. Genetic fingerprinting of β-lactamase producing K. pneumoniae using ERIC-PCR and REP-PCR differentiated all the 32 strains with discriminatory power of 1.

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“…DNA was extracted by boiling and snap chilling method (Suresh et al, 2018) from all the PCT positive P. mirabilis isolates and subjected to PCR assays for detection of ESBL genes (Table 1 and 2). PCR assays were optimized in 25 μl reaction mixture containing 2 μl of DNA template, 12.5 μl of 2x master mix (Go Taq Green Master Mix, Promega), 0.5 μl each of forward and reverse primers (10 pmol/μl) and the rest of the volume is made by adding nuclease free water, under standardized cycling conditions: initial denaturation…”

Section: Genotypic Detection Of β-Lactamase Genesmentioning

confidence: 99%

A Study on Antibiogram and Beta-lactam Resistance of Proteus mirabilis Isolated from Animals and Humans in Andhra Pradesh, India

Ram¹,

Rao²,

Rao³

et al. 2021

IJAR

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Background: Proteus mirabilis is one of the organisms which is often associated with urinary tract infections of humans as well as animals. As a member of Enterobacteriaceae family, the level of antimicrobial resistance tend to pose a significant public health risk. Hence, the present study was undertaken to study antibiogram profiles, multidrug resistance P. mirabilis isolates and detection of β-lactamase activity in them.Methods: A total of 175 P. mirabilis isolates from different sources were subjected to antibiotic sensitivity/ resistant test by Kirby Bauer disc diffusion method. Detection of ESBL production was done phenotypically by Phenotypic Screening Test and Phenotypic Confirmation Test as recommended by CLSI guidelines and genotypically using multiplex PCR assay to detect different classes of β-lactamase genes. This study was carried out from March 2017 to August 2018 in and around areas of Krishna District Andhra Pradesh.Result: Out of 175 P. mirabilis isolates screened, antibiogram revealed highest sensitivity towards gentamicin (76.57%), followed by ampicillin (64.57%), kanamycin (61.14%), amikacin (60.57%) and streptomycin (43.42%). Higher resistance was observed for erythromycin (71.42%), nalidixic acid (62.85%), ciprofloxacin (62.85%), tetracycline (60%), polymyxin-B (60%), cefoxitin (49.14%) and amikacin (36%). β-lactamase genes were detected in a total of 23 isolates (13.14%). Prevalence rates of β-lactamase genes among different samples was 23.6%, 11.1%, 10.8% and 42.8% from chicken, pork, poultry cloacal swabs and human urine samples, respectively with blaTEM being the predominant gene detected (69.56%) followed by blaOXA (26.08%), blaAmpC gene FOX (13.04%), blaCTX-M group I (4.34%), blaSHV (4.34%) and blaAmpC gene CIT (4.34%) among all the tested P. mirabilis isolates.

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Isolation, Molecular Characterization and Antimicrobial Resistance Patterns of Four Different Vibrio Species Isolated from Fresh Water Fishes

Cited by 9 publications

References 26 publications

Microbiological and molecular characterization of Vibrio cholera and Vibrio parahaemolyticus isolated from Tilapia fish (Oreochromis niloticus

Microbiological and molecular characterization of Vibrio cholera and Vibrio parahaemolyticus isolated from Tilapia fish (Oreochromis niloticus

Occurrence and genetic diversity of ESBL producing Klebsiella species isolated from livestock and livestock products

A Study on Antibiogram and Beta-lactam Resistance of Proteus mirabilis Isolated from Animals and Humans in Andhra Pradesh, India

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