Occurrence and genetic diversity of ESBL producing <i>Klebsiella</i> species isolated from livestock and livestock products

Bobbadi, Suresh; Chinnam, B. K.; Nelapati, Subhashini; Tumati, Srinivasa Rao; Kandhan, Srinivas; Gottapu, Chaitanya; Boddu, Swathi V.

doi:10.1111/jfs.12738

Cited by 10 publications

(5 citation statements)

References 19 publications

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“…3) by PCR. In a study conducted by Bobbadi et al (2020) in Andhra Pradesh, 90 per cent of K. pneumoniae isolates were confirmed by PCR targeting rpoB gene.…”

Section: Resultsmentioning

confidence: 99%

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Occurrence of Klebsiella pneumoniae and Salmonella spp. in environmental samples

Swetha¹,

Mathew²,

Latha³

et al. 2022

Journal of Veterinary and Animal Sciences

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The present study was undertaken for the isolation and identification of Klebsiella pneumoniae and Salmonella spp. from environmental samples. A total of 120 soil and 120 water samples were collected from ponds and rivers in Thrissur. The samples were subjected to conventional culture techniques and K. pneumoniae and Salmonella spp. isolates were detected in 57.50 and 14.58 per cent samples, respectively.There were no significant differences between the occurrence of K. pneumoniae and Salmonella spp. in different soil and water samples, (p≥0.05). The isolates were subjected to molecular confirmation targeting rpoB gene for K. pneumoniae and invA gene for Salmonella spp. Out of the 138 K. pneumoniae and 35 Salmonella spp. isolates analysed, 86.96 and 97.14 per cent isolates were found positive on PCR. The results of the study revealed that environment acts as an important reservoir of K. pneumoniae and Salmonella spp.

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“…3) by PCR. In a study conducted by Bobbadi et al (2020) in Andhra Pradesh, 90 per cent of K. pneumoniae isolates were confirmed by PCR targeting rpoB gene.…”

Section: Resultsmentioning

confidence: 99%

“…The isolates were further subjected to molecular confirmation by polymerase chain reaction (PCR) targeting rpoB gene for K. pneumoniae (Bobbadi et al, 2020) and invA gene for Salmonella spp. (Amini et al, 2010).…”

Section: Methodsmentioning

confidence: 99%

Occurrence of Klebsiella pneumoniae and Salmonella spp. in environmental samples

Swetha¹,

Mathew²,

Latha³

et al. 2022

Journal of Veterinary and Animal Sciences

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“…In the current study, we did not detect bla SHV . The dissemination of bla CTX-M-2 among K. oxytoca isolates collected from pigs has been reported [21,22], and bla CTX-M-15 and bla TEM-1 were detected in K. oxytoca isolates collected from six provinces in China [23]. K. oxytoca isolates harboring bla CTX-M were highly susceptible to cefotaxime, ceftazidime, and cefepime (93.1-98.3%) and 100% susceptible to ceftriaxone.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and molecular characterization of β-lactamase and plasmid-mediated quinolone resistance genes in Klebsiella oxytoca isolated from slaughtered pigs in Thailand

et al. 2022

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Background and Aim: Over recent years, antimicrobial-resistant Klebsiella species in humans, animals, food animals, food products, and agricultural environments have been the center of attention due to its role in the evolution of antimicrobial resistance. The emergence of resistance to fluoroquinolones and cephalosporins of third and higher generations in Klebsiella oxytoca has not received much attention in animal husbandry compared to that in Klebsiella pneumoniae. Reports on K. oxytoca are limited in the study area. Therefore, we investigated the antimicrobial susceptibility and resistance genes in K. oxytoca isolated from slaughtered pigs in Thailand. Materials and Methods: Microbiological examination was conducted on 384 Klebsiella spp. isolates recovered from slaughtered pigs in ten provinces of Thailand. Seventy-two K. oxytoca isolates (18.75%) were examined for antimicrobial-resistant genes (β-lactamase [blaTEM, blaCTX-M, and blaSHV]) and fluoroquinolone-resistant genes (qnrA, qnrB, qnrC, qnrD, qnrS, oqxAB, aac(6')-Ib-cr, and qepA). Results: The most common genotype was blaCTX-M (58/72, 80.55%), followed by blaTEM with blaCTX-M (7/72, 9.72%) and blaTEM (6/72, 8.33%). The most common blaCTX-M group was blaCTX-M-1 (19/58, 32.76%), followed by blaCTX-M-9 (1/58, 1.72%). Plasmid-mediated quinolone resistance genes were identified in 13 (18.05%) isolates: qnrS (16.70%) and qnrB (1.4%). All 13 isolates had qnrS transferable to an Escherichia coli recipient, whereas qnrB was not detected in any transconjugants. Either blaCTX-M or blaTEM harbored by one K. oxytoca strain was transferable to an E. coli recipient. Analysis of antimicrobial susceptibility revealed that more than 90% of the blaCTX-M-carrying K. oxytoca isolates were susceptible to chloramphenicol, trimethoprim, ceftazidime, cefepime, cefotaxime, amoxicillin-clavulanic acid, piperacillin–tazobactam, and fosfomycin. All K. oxytoca isolates (13) harboring qnr were susceptible to carbapenem and ceftriaxone; however, 43 (74.13%) of the K. oxytoca isolates harboring blaCTX-M exhibited extended-spectrum β-lactamase activity. Most of the K. oxytoca isolates from pigs were highly resistant to ampicillin, azithromycin, and gentamicin. Conclusion: To prevent further transmission of Klebsiella spp. between food animals and humans, strict control of antibiotic use in clinical and livestock settings is necessary along with routine disinfection of the livestock environment and efforts to increase awareness of antimicrobial resistance transmission.

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“…The ERIC-PCR-based fingerprinting method has been used for the relatedness or differentiation analysis of pathogenic bacteria, including K. pneumoniae [ 55 ]. For instance, Zhang et al [ 41 ] reported 60 ERIC genotypes among 61 K. pneumoniae strains from 1200 retail foods sampled from May 2013 to April 2014 in China [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

First Report of Potentially Pathogenic Klebsiella pneumoniae from Serotype K2 in Mollusk Tegillarca granosa and Genetic Diversity of Klebsiella pneumoniae in 14 Species of Edible Aquatic Animals

Guan

et al. 2022

Foods

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Klebsiella pneumoniae can cause serious pneumonitis in humans. The bacterium is also the common causative agent of hospital-acquired multidrug-resistant (MDR) infections. Here we for the first time reported the genetic diversity of K. pneumoniae strains in 14 species of edible aquatic animals sampled in the summer of 2018 and 2019 in Shanghai, China. Virulence-related genes were present in the K. pneumoniae strains (n = 94), including the entB (98.9%), mrkD (85.1%), fimH (50.0%), and ybtA (14.9%) strains. Resistance to sulfamethoxazole-trimethoprim was the most prevalent (52.1%), followed by chloramphenicol (31.9%), and tetracycline (27.7%), among the strains, wherein 34.0% had MDR phenotypes. Meanwhile, most strains were tolerant to heavy metals Cu2+ (96.8%), Cr3+ (96.8%), Zn2+ (91.5%), Pb2+ (89.4%), and Hg2+ (81.9%). Remarkably, a higher abundance of the bacterium was found in bottom-dwelling aquatic animals, among which mollusk Tegillarca granosa contained K. pneumoniae 8-2-5-4 isolate from serotype K2 (ST-2026). Genome features of the potentially pathogenic isolate were characterized. The enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR)–based genome fingerprinting classified the 94 K. pneumoniae strains into 76 ERIC genotypes with 63 singletons, demonstrating considerable genetic diversity in the strains. The findings of this study fill the gap in the risk assessment of K. pneumoniae in edible aquatic animals.

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Occurrence and genetic diversity of ESBL producing Klebsiella species isolated from livestock and livestock products

Cited by 10 publications

References 19 publications

Occurrence of Klebsiella pneumoniae and Salmonella spp. in environmental samples

Occurrence of Klebsiella pneumoniae and Salmonella spp. in environmental samples

Phenotypic and molecular characterization of β-lactamase and plasmid-mediated quinolone resistance genes in Klebsiella oxytoca isolated from slaughtered pigs in Thailand

First Report of Potentially Pathogenic Klebsiella pneumoniae from Serotype K2 in Mollusk Tegillarca granosa and Genetic Diversity of Klebsiella pneumoniae in 14 Species of Edible Aquatic Animals

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