Isolation, identification, characterization and antibiogram of Pasteurella multocida isolated from pigs in Mizoram with special reference to progressive atrophic rhinitis

Abstract

How to cite this article: Varte Z, Dutta TK, Roychoudhury P, Begum J and Chandra R (2014) Isolation, identification, characterization and antibiogram of Pasteurella multocida isolated from pigs in Mizoram with special reference to progressive atrophic rhinitis, Veterinary World 7(2): 95-99. Introductionfor induction of the experimental disease, development of improved diagnostics, development of effective Progressive atrophic rhinitis (PAR) is a widely therapeutic/ prophylactic pharmaceutical approaches prevalent, upper respiratory tract disease of swine and development of immunoprophylactic products for characterized by sneezing, snuffling, nasal discharge, effective control of the disease. epistaxis, growth retardation, reduction in feed There is paucity of information regarding PAR in utilization, degeneration and atrophy of the nasal pigs in India, particularly in North Eastern Region. turbinate bone leading to visible distortion and Therefore, the present study was conducted for shortening of the snout [1]. Moreover, it has been isolation and identification of P. multocida from nasal recognized as a contributor to debilitating and fatal cavities of pigs of Mizoram with or without clinical porcine pneumonia for at least 120 years and continues lesions of PAR followed by their detection, capsular to be sustained, unabated with high prevalence in typing and screening for toxA gene by PCR based different part of the world [2]. Toxigenic strains of P.assays. multocida (both capsular type A and D) alone, or pigs, 9 were showing respiratory distress as a suspected Amongst the livestock, pig is most important and case of atrophic rhinitis. Samples were collected from almost every family rears pig as backyard venture.local household pigs as well as from organized pig Since the pig rearing is an important sector in rural farms. economy in this region, a better understanding of PAR Detection of P. multocida by PCR assay: Each swab and its impact on animal husbandry and economy is was grown in Brain-Heart Infusion (BHI) broth essential. Studies on PAR should provide a foundation o overnight at 37 C. After incubation, 1 ml of bacterial suspension was centrifuged at 7084 g. The bacterial pellet was washed thrice with sterile normal saline solution (NSS) (0.85% w/v) and resuspended in 300 µl Materials and Methods:Four hundred nasal swabs sampled from pigs were collected from 6 different districts of Mizoram. Swabs were processed for detection and isolataion of P. multocida by PCR and traditional bacteriological assays. Isolates were subjected for multiplex PCR for capsular typing, detection of toxA gene and characterization by RAPD-PCR. Isolates were also tested for antimicrobial sensitivity profile by disc-diffusion method.Results: A total of 21 swabs were found to be positive by P. multocida species specific PCR (PM-PCR) with an amplicon of 460 bp, of which P. multocida could be isolated from 15 swabs (3.75%). All the isolates were grouped under capsular type A (n=9) and D (n=6) by multiplex PCR. All the isolates were ...

Use of molecular biology tools for rapid identification and characterization of Pasteurella spp.

“…Pasteurellosis confirmation seems to be difficult due to multiple clinical symptoms and time-consuming laboratory procedures. Furthermore, it is difficult to obtain a pure culture of PM from clinical isolates because of contaminants and/or death of organisms [ 26 ]. It has been observed that the detection of PM in the clinical material is greatly accelerated by the use of PCR technique and could yield isolation of the organism.…”

Virulence gene profiling of porcine Pasteurella multocida isolates of Assam

“…Pasteurellosis is a common disease of pigs worldwide with specific serotype and pathotype associated with the respiratory disease [ 16 - 18 ]. However, the distribution pattern of the serotypes and pathotypes can vary considerably from region to region and over time in a given region [ 12 , 19 ]. The present study results in the detection of more percentage of serotype A than serotype D. The isolates used in the present study were mainly from cases of the respiratory syndrome.…”

“…A higher percentage of serotype A than serotype D in P. multocida isolates from indigenous pigs in central Kalorey et al . [ 20 ] and Northeast India [ 19 , 21 ] have been reported.…”

Prevalence, the antibiogram and the frequency of virulence genes of the most predominant bacterial pathogens incriminated in calf pneumonia

“…Further, the toxA gene is a virulence gene associated with P. multocida, was identified by PCR in all isolates. A previous investigation reported that PCR targeting toxA gene is sensitive and essential for the detection of pathogenic P. multocida (Varte et al 2014).…”

“…The P. multocida strains were exposed to the pathogenicity test as the previously described protocol (Varte et al 2014). Briefly, six mice were I/P inoculated with 100 μl of the whole culture; in addition, three mice were inoculated with 100 μl of sterile H 2 O (control group).…”