Isolation, Identification and Characterization of Enteric Bacteria from Post Weaning Diarrheic Pigs and their Resistance to Multiple Antibiotics

Mittal, Dinesh; Grakh, Kushal; Prakash, Anand; Moudgil, Pallavi; Devi, Bhanita; Jadhav, Vijay J.

doi:10.20546/ijcmas.2018.712.269

Cited by 3 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In support of our study, Londhe (2010) [10] also reported the resistance of E. coli to cephalosporin but in contrast to our study, Londhe (2010) [10] and Lehreena (2008) [11] reported the sensitivity of E. coli to polymyxins and cephalosporin/β-lactamase inhibitors, respectively. More or less similar findings were reported by Dinesh et al (2018) [12] regarding the resistance of E. coli to penicillin+β-lactamase inhibitors and aminoglycosides, but in contrast to our study, they found that E. coli showed sensitivity towards fluoroquinolone. Brennan et al (2016) [13] , in their study in bovine, found that one isolate of E. coli was resistant to the β-lactamase and fluoroquinolone groups of antibiotics.…”

Section: Discussionsupporting

confidence: 88%

Detection of multidrug-resistant Enterobacterales isolated from buffalo species

Lather,

Kumar

et al. 2024

Int. J. Adv. Biochem. Res.

View full text Add to dashboard Cite

The study was conducted to investigate the detection of multidrug-resistant Enterobacterales isolated in buffalo species during the period of August, 2019 to January, 2020 at the Department of Veterinary Pathology, LUVAS, Hisar. For bacteriological isolation, the samples were collected aseptically from the heart blood, liver, and mesenteric lymph nodes of buffalo species suspected of gastrointestinal tract disorders. The bacterial species were identified through the Vitek-2 Compact system. Out of 31 Gram negative bacteria, a total 17 different bacterial species were identified. Most of the bacterial species showed multiple drug resistance patterns.

show abstract

Section: Discussionsupporting

confidence: 88%

Detection of multidrug-resistant Enterobacterales isolated from buffalo species

Lather,

Kumar

et al. 2024

Int. J. Adv. Biochem. Res.

View full text Add to dashboard Cite

show abstract

“…These E. coli isolates were con rmed using Vitek 2 Compact system. Gram negative (GN) reagent cards were used for identi cation of E. coli as per the recommendation of the manufacturer and the method described previously (Mittal et al 2018).…”

Section: Source Of Samplesmentioning

confidence: 99%

Characterization and antimicrobial sensitivity of biofilm producing Avian Pathogenic Escherichia coli from broiler chickens and their environment in India.

Grakh

Mittal

Prakash

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Avian pathogenic Escherichia coli (APEC) is responsible for colibacillosis in poultry. APEC remains a constant problem for the poultry industry, despite the use of antibiotics and disinfectants at farms. The endemicity of APEC in poultry farms is associated with its biofilm forming ability, which is further aggravated by various virulence factors harbored by this pathogen and resistant to multiple drugs which help bacteria to thrive under different environmental conditions. To characterize APEC from affected broiler chickens and their environments, samples (n=114) were collected and subjected to microbial isolation using MacConkey Lactose agar (MLA) and Eosin Methylene Blue agar (EMB), which led to the isolation of 62 E. coli isolates, confirmed using uspA gene amplification and Vitek 2 Compact system. These isolates were characterized using a set of five virulence genes (hlyF, ompT, iroN, iss, iutA) which yielded 47 (75.80%) isolates as APEC and the remaining as non-APEC. Furthermore, all the 62 isolates were subjected to microtiter plate assay for biofilm detection and the result showed that 36 (58.06%) isolates were able to form moderate to strong biofilms in Trypticase soy broth (TSB) at 72h of incubation. Of the 36-biofilm forming isolates, 30 were APEC. Biofilm related genes (crl, csgA, fimH, luxS, and papC) were also detected with higher prevalence among APEC isolates. Antimicrobial sensitivity test using Vitek2 system revealed 43 (91.48%) of 47 APEC isolates as multiple drug resistant (MDR) and 8 (17.02%) as ESBL positive. This study reveals that APEC with biofilm formation ability are present in poultry farms. Further studies are needed to understand the role of biofilms in the pathogenesis and antimicrobial resistance of APEC.

show abstract