Isolation, Growth and Identification of Chlorpyrifos Degrading Bacteria from Agricultural Soil in Anambra State, Nigeria

Ifediegwu, M C; Agu, Kingsley Chukwuebuka; Awah, N. S.; Mbachu, A. E.; Okeke, CU; Anaukwu, Chikodili Gladys; Uba, P.O.; Ngenegbo, U.C.; Nwankwo, C.M.

doi:10.13189/ujmr.2015.030402

Cited by 10 publications

(5 citation statements)

References 11 publications

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“…and especially P. aeruginosa reduce the concentration of chlorpyrifos concentration by 42%. Ifediegwu et al (2015) also stated that the reduction in chlorpyrifos concentration by P. aeruginosa was 60%. Xia et al (2013) showed that Serratia sp.…”

Section: Discussionmentioning

confidence: 99%

The role of bacterial symbionts in the biodegradation of chlorpyrifos in the digestive tract of Plutella xylostella larvae

et al. 2021

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Abstract. Hadi MS, Abadi AL, Himawan T, MAsruri, Lestari SR, Rahardjo BT, Aini LQ, Setiawan Y, Tarno H. 2021. The role of bacterial symbionts in the biodegradation of chlorpyrifos in the digestive tract of Plutella xylostella larvae. Biodiversitas 22: 702-712. Several species in the order Lepidoptera act as plant pests, one of which is Plutella xylostella. Plutella xylostella is one of the most destructive pests of cabbage and other horticultural crops. The use of chemical insecticides as pest control for P. xylostella causes many problems, such as the increased pest resistance to pesticides. The objectives of this study are: (i) to obtain and characterize symbiont bacteria in the digestive tract of P. xylostella collected from organic and conventional agriculture soils; (ii) to evaluate the potential of bacterial symbionts in the digestive tract of P. xylostella from organic and conventional soils in degrading the active ingredient of chlorpyrifos insecticide; (iii) To determine the biodegradation process of chlorpyrifos insecticide by symbiont bacteria in the digestive tract of P. xylostella; and (iv) to identify the derivative compounds from the biodegradation of chlorpyrifos insecticide. The results showed 30 symbiont bacteria isolated from the digestive tract of P. xylostella collected from organic soil and 36 symbiont bacteria isolated from the digestive tract of P. xylostella from conventional farming soil. There are 15 species of symbiont bacteria in 5 genera from the digestive tract of P. xylostella from organic and conventional farming capable of degrading the chlorpyrifos insecticide. They are identified as Providencia sp., Pseudomonas sp., Serratia sp., Proteus sp., and Aeromonas sp. Chlorpyrifos-derived compounds from the biodegradation of symbiont bacteria are less toxic than chlorpyrifos compounds.

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Section: Discussionmentioning

confidence: 99%

The role of bacterial symbionts in the biodegradation of chlorpyrifos in the digestive tract of Plutella xylostella larvae

et al. 2021

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“…The flasks were incubated on a rotary shaker at 150rpm at 30 0 C for 28 days. The optical density of the isolates was determined at intervals of 4 days for 28 days using Spectrophotometer (OD 600nm) as described by [2].…”

Section: Molecular Identification Of Isolatesmentioning

confidence: 99%

“…, used on grain, cotton, fruit, and vegetable crops, as well as, lawns and ornamental plants [2], against common pests like moths, hoppers, midges, worms and spiders [3]. The use of Pesticide has greatly improved crop productivity and reduced reduction of crop yield.…”

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confidence: 99%

Isolation, Identification and Characterization of Bacteria Capable of Degrading Chlorpyrifos from Agricultural Soil at Amansea, Anambra State Nigeria.

Chiemeka Elochi,

Samuel Chinedu,

Chinonyelum Dilys

2024

IDOSR JBCP

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Chlorpyrifos (O, O-diethyl O-(3,5,6-trichloro-2-pyridyl phosphorothioate) a broad spectrum moderately toxic organophosphorous insecticide is commonly used as pest control on grain, cotton, fruits, and vegetable crops against pest. Although it is important, its use has become one of the major causes of soil and water pollution. The present technique for removing chloryrifos insecticide from the environment which is biodegradation has shown to be more effective than any other physical or chemical methods like chemical hydrolysis. Therefore the aim of this research was to evaluate the biodegradation of chlorpyrifos insecticide by bacteria isolated from agricultural soil in Amansea, Anambra State, Nigeria. In the present study, two bacteria capable of degrading chlorpyrifos were isolated from Agricultural soil using mineral salts medium and characterized based on their physiological, biochemical, morphological and 16S rRNA sequencing as strains of Bacillus cereus ST06 and Chryseobacterium sp. 6024. Their growth response in mineral salts medium supplemented with 20mg/l concentration of chlorpyrifos was determined by monitoring the optical density at 600nm and at optimium condition of pH 6.5 and 300C for 28 days. The residual chlorpyrifos concentration after 28 days was also determined using Gas Chromatography- Electron Cathode Detector (GC-ECD). The result from one way ANOVA indicated that there were significant differences (P < 0.05) in the Growth responses to the insecticides by the test organisms. The result showed that Bacillus cereus ST06 and Chryseobacterium sp. 6024 reached maximum growth on 20mg/l chlorpyrifos with an arithmetic mean difference of 0.23±0.20 and 0.42±0.02 respectively on day 16 .There were also significant (<0.05) difference in concentration of residual chlorpyrifos degraded by the isolates obtained from the GC-ECD curves. Bacillus cereus ST06 and Chryseobacterium sp. 6024 degraded 63% and 57% of 20mg/l chlorpyrifos respectively. Bacillus cereus ST06 showed better results and possess potential to be used in biodegradation of 20mg/l Chlorpyrifos than Chryseobacterium sp. 6024. It is therefore recommended that further studies on the effect of varying Chlorpyrifos concentration on the bacteria singly and as a consortium be studied to better understand their potential for degrading chlorpyrifos efficiently.Keywords: Isolation, Identification, Characterization, Bacteria, Biodegradation, Chlorpyrifos and Insecticides

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“…Organophosphate pesticides have been linked to various health effects, including cancer, nausea, and headaches, and are associated with endocrine disruption, reproductive health concerns, and neurotoxic effects with long-term exposure (Geed et al, 2017). Organophosphate residues in food are linked to various cases of food poisoning recorded in different parts of the world (Ifediegwu et al, 2015;Uniyal et al, 2021;Ambreen & Yasmin, 2021). Milk is an ideal food for dissolving organic pollutants, including pesticides, due to its lipophilic properties (Nasef et al, 2019;Rêgo et al, 2019).…”

Section: Impact Of Organophosphate Residues On Healthmentioning

confidence: 99%

Organophosphate Pesticide Residues in Food Products in Kenya and their Chromatographic Detection: A Systematic Review

Asamba

Wepukhulu

2023

East Afr. j. agric. biotechnol.

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Organophosphate pesticides are used worldwide to control several pests and meet food demand. These chemicals harm non-target animals and people when misused. Thus, they are a health and environmental concern. The purpose of the systematic review was to synthesise the amount, breadth, and quality of evidence from empirical studies concerning the presence, type, and quantity of OPs in food products in Kenya. A systematic review was done by following the PRISMA protocol. For the identification of studies, the following databases were used: Google Scholar, Web of Science, Scopus, and PubMed. The study focused on peer-reviewed articles published between January 2001 and August 2022. Twelve studies met the inclusion criteria for the scoping review. The main methods used in detecting and quantifying organophosphates in the studies were High-Performance Liquid Chromatography (HPLC) and Gas Chromatography-Mass Spectrometry (GC-MS). Most studies (86.67%) reported significant levels of OPs in food products, exceeding the MRLs and the Acceptable Daily Intake (ADIs) set by the World Health Organization. Six of the studies (50%) reported the presence of OPs in plant products (vegetables, cereals, and fruits), while three (25%) found residues in animal products (milk and meat). The most notable OP compounds detected were chlorpyrifos, acephate, profenofos, diazinon, omethoate, and dimethoate. Most of the food samples in the reviewed studies presented contamination, making them a significant risk to human health due to bioaccumulation. Studies done in Kenya for the last 20 years continue to report high levels of organophosphate residues and their metabolites in food products, both from plant and animal origin. Although the residues are below MLRs in some samples, they can accumulate at higher levels in humans, becoming a severe health risk

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Isolation, Growth and Identification of Chlorpyrifos Degrading Bacteria from Agricultural Soil in Anambra State, Nigeria

Cited by 10 publications

References 11 publications

The role of bacterial symbionts in the biodegradation of chlorpyrifos in the digestive tract of Plutella xylostella larvae

The role of bacterial symbionts in the biodegradation of chlorpyrifos in the digestive tract of Plutella xylostella larvae

Isolation, Identification and Characterization of Bacteria Capable of Degrading Chlorpyrifos from Agricultural Soil at Amansea, Anambra State Nigeria.

Organophosphate Pesticide Residues in Food Products in Kenya and their Chromatographic Detection: A Systematic Review

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