The major control of pest stored Lasioderma serricorne for using synthetic pesticides, look like phosphine. Although quite effective, synthetic pesticides have a negative effect on the environment, such as pest resistance, deadly natural enemies, residues that are harmful to the environment and human health. The use of botanical pesticides as an alternative control be the focus this study. Botanical pesticides are selective to the target, safe for non-target insects and the environment. This research studies the repellent and fumigant activity of the leaf extract of Citrus hystrix against all L. serricorne life stage. The results of GC-MS analysis of leaf crude extracts of C. hystrix with N-hexane solvent showed the highest percentage in the citronellal compound (86.43%). C. hystrix leaf extract showed stronger fumigant against pupae and eggs, compared adults, and larvae. The toxicity of the leaf extract of C. hystrix increased with increasing concentration. At a concentration of 60 ppm, fumigant activity showed the toxicity of 98.75% (pupae), 93.75% (eggs), 86.25% (adults) and 76.25% (larvae). Sequentially the LC50 value of fumigant activity from the highest to the lowest as follows; larvae 47.56 ppm, adults 43.42 ppm, eggs 31.61 and pupae 29.63 ppm. Extract of leaves of C. hystrix, have character repellent against L. serricorne. At a concentration of 60 ppm the IR value of 66% including repellent class IV, which means strong repellent level. Based on the results of the research, extracts of leaves of C. hystrix has a fumigant activity and repellent for controlling L. serricorne.

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Sonokembang (Pterocarpus indicus Willd.) is native of Asia trees, and commonly planted in large numbers as shade trees along roads in Malang. Research was conducted on 76 roads to identify damage intensity of dying trees on sonokembang and clarify the causal agent of dying trees in Malang from May to December 2012. Seven variables was also investigated such as characteristic of tree`s damage, distribution of holes based on sunlight exposure, vertical position of stem, and stem diameter, morphological characteristic of beetle, the intensity of dying trees, and distribution of dying trees. Results showed that there were unique damaging characteristic such as dying and then fallen leaves, holes on the stem and branches, and frass production. Euplatypus parallelus Fabricius was identified as a causal agent of dying sonokembang. There were some indications shown such as beetles preferred to attack stem side with much sunlight exposure, upper stem and medium or bigger size of stem diameter. From 3,206 trees on 76 roads, 69.7% were found dying trees. In dry and rainy season, the intensity of dying trees increased from 8.14 to 9.76% and from 10.26 to 10.79%, respectively.

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Abstract. Tarno H, Octavia E, Himawan T, Setiawan Y. 2022. Detection of fruit flies (Diptera: Tephritidae) Using Cue-Lure and Methyl Eugenol in Steiner Traps in Depok City and Bogor District, West Java, Indonesia. Biodiversitas 23: 4202-4208. Fruit flies (Diptera: Tephritidae) are one of the most economically important insect pests worldwide. Two types of attractants are recommended for monitoring and controlling fruit flies: methyl eugenol (ME) and cue-lure (CL). The objective of this study was to investigate the species richness and abundance of fruit flies (Diptera: Tephritidae) using methyl eugenol (ME) and cue-lure (CL) in Depok City and Bogor District, West Java, Indonesia. The study was conducted in three sites of fruit orchards in Bogor District i.e., Malay apple (Syzygium malaccense), Lemon (Citrus limon), Papaya (Carica papaya) and two sites of fruit orchards in Depok District i.e., carambola (Averrhoa carambola) and guavas (Psidium guajava). Male fruit flies were trapped using Steiner traps baited with methyl eugenol (ME) and cue-lure (CL). There were 11 fruit fly species collected from this study i.e., Bactrocera albistrigata, B. carambolae, B. dorsalis, B. neocognata, B. umbrosa, B. verbascifoliae, Dacus conopsoides, D. longicornis, Zeugodacus calumniata, Z. caudatus, and Z. cucurbitae. In this study, B. was , D. conopsoides, D. longicornis, and Z. caudatus were only collected in Steiner traps baited with CL and B. dorsalis and B. umbrosa also only responded to ME. During sampling periods, 20,217 male adult fruit flies were collected. The highest relative abundance attracted to ME was B. carambolae and CL was Z. cucurbitae.  In this study, B. albistrigata, B. calumniate, D. longicornis, Z. caudatus and Z. cucurbitae were significantly more attracted to CL than ME. Whereas, B. carambolae, B. dorsalis, B. umbrosa, and B. verbascifoliae were significantly more attracted to ME than CL. Our study showed also did not find invasive species in Depok City and Bogor District, West Java, Indonesia. Our study can be used as a method for monitoring and controlling fruit flies, namely Steiner trap with CL for Z. cucurbitae and Steiner trap with ME for B. carambolae, B. dorsalis and B. umbrosa in Depok City and Bogor District, West Java, Indonesia.

This study was to determine the fruit maturity level of wild bitter gourd and the composition of water extract which is effective as antifeedant and appropriate for mortality test on armyworm larvae. Results obtained show that water extract of wild bitter gourd fruit contains phenolic compounds, flavonoids and triterpenoids. Terpenoid compounds contained in water extract of fruits at maturity level 4H, 8H and 12H were momordicoside L, momordicoside K, compound 3β, β7, 25-trihydroxycucurbita-5.23(E)-diena-19-al:R1=H, R2= H and momordicine 1. Fruit maturity level 4H 50% and 4H 60% resulted to the highest antifeedant index, ie 40.08% and 44.20%. LC 50 at fruit maturity level 4H observed on day 7 was 40%.

Natural enemy is an important factor in management of cacao pests. One way to increase its diversity and abundance is through agroecosystem management techniques that support. The study was conducted for one year from February 2014 to February 2015 in Banjarsari Plantation and a cocoa farm of Kaliwining Experimental Station, Jember, which applied different cropping patterns and use of insecticides. The purpose of this research was to study the effect of cropping pattern and use of insecticide on diversity and abundance of predators, parasitoids, abundance and intensity of Helopeltis antonii attack. Observation of pests and natural enemies was done visually, using sweep net, yellow pan traps, malaise trap and pifall trap. Intensity of the attack used Pedigo & Buntin equations. Diversity index used Shannon Diversity Index (H’) and Simpson’s dominance index (C). Differences in predator abundance, parasitoid, air temperature, relative humidity, light intensity and rainfall used Wilcoxon test. Results of this study showed that monoculture and intensive use of synthetic insecticides caused lower diversity and abundance of predators and parasitoids, while the abundance and intensity of H. antonii attacks were higher compared with polyculture without insecticide. Banjarsari cocoa plantation that applied monoculture and intensive use of synthetic insecticides, had lower diversity and abundance predators and parasitoids compared with cocoa plantation in Kaliwining that applied polyculture planting without using insecticide. Dominant predators in Banjarsari and in Kaliwining cocoa plantations were Dolichoderus thoracicus and Araneus diadematus, while dominant parasitoid in Banjarsari and in Kaliwining cocoa plantations was Araneus diadematus.

Abstract. Ikawati S, Himawan T, Abadi AL, Tarno H. 2020. Thermostability, photostability, and toxicity of clove oil nanoparticles against Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae). Biodiversitas 21: 4764-4771. Problems related to the essential oils as insecticides are their volatility, poor solubility in water and environmental degradation (such as caused by high temperatures and UV light) which can adversely affect the application. An ideal botanical insecticide formulation would maintain the level of the main compounds adequate for pest control. This study was to evaluate the stability of polyethylene glycol (PEG) coated nanoparticles loaded with clove (Syzygium aromaticum) essential oil to high temperature and UV light also their toxicity to C. ferrugineus. There is a reduction in nanoparticle size after heating from 257 to 173 nm and there is no difference in size distribution. After heating and UV-light treatment, eugenol content in free clove oil decreased significantly, while on nanoparticles there were not significantly changed, that indicated greater stability on the nanoformulation. The median lethal concentration (LC50) of CO-NPs to C. ferrugineus adults value for 72 and 120 hours was 0.734 and 0.676%.