Isolation, characterization, antibiogram and pathology of Pasteurella multocida isolated from pigs

Tigga, Mamta; Ghosh, R. C.; Malik, Praveen; Choudhary, B.K.; Tigga, Pratap; Nagar, Dinesh Kumar

doi:10.14202/vetworld.2014.363-368

Cited by 10 publications

(12 citation statements)

References 8 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The respiratory system of many animal species including pigs is inhabited with commensal organism Pasteurella multocida , which is considered opportunistic pathogens associated with diseases such as atrophic rhinitis [ 3 ] and pneumonic pasteurellosis [ 4 ] in pigs. The systemic immune response against such infection has been studied extensively.…”

Section: Introductionmentioning

confidence: 99%

Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine

George¹,

Barman²,

Nath³

et al. 2015

Vet World

View full text Add to dashboard Cite

Aim:The aim was to study the bronchoalveolar lavage (BAL) technique in evaluating the local immune response of pig immunized with Pasteurella multocida bacterin vaccine.Materials and Methods:Weaned piglets were immunized with formalin-inactivated P52 strain of P. multocida bacterin and evaluated for pulmonary immune response in BAL fluid. BAL was performed before vaccination and at different post vaccination days. The BAL fluid was assayed using enzyme-linked immunosorbent assay to study the development of P. multocida specific antibody isotypes and also evaluated for different cell populations using standard protocol.Results:The average recovery percentage of BAL fluid varies from 58.33 to 61.33 in vaccinated and control group of piglets. The BAL fluid of vaccinated pigs showed increase in antibody titer up to 60th days post vaccination (8.98±0.33), IgG being the predominant isotype reached maximum titer of 6.12±0.20 on 45th days post vaccination, followed by IgM and a meager concentration of IgA could be detected. An increased concentration of the lymphocyte population and induction of plasma cells was detected in the BAL fluid of vaccinated pigs.Conclusion:Though intranasal vaccination with P. multocida plain bacterin vaccine could not provoke a strong immune response, but is promising as lymphocyte population was increased and plasma cells were detected. BAL can be performed repeatedly up to 3/4 months of age in pigs to study pulmonary immune response without affecting their health.

show abstract

Section: Introductionmentioning

confidence: 99%

Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine

George¹,

Barman²,

Nath³

et al. 2015

Vet World

View full text Add to dashboard Cite

show abstract

“…Beside it, small amount of frothy exudate in trachea and hydrothorax ( Figure-2 ) was also noted as found by Tigga et al . [ 11 ] and Harish et al . [ 9 ].…”

Section: Resultsmentioning

confidence: 99%

“…Similar histological lesions in lungs were observed by Tigga et al . [ 11 ]. The lesions such as bronchioles filled with necrotic exudates containing inflammatory cells and multifocal hemorrhages in the interstitial spaces ( Figure-7 ) were in accordance with Harish et al .…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Histopathological and immunohistochemical approaches for the diagnosis of Pasteurellosis in swine population of Punjab

Bhat¹,

Singh²,

Leishangthem³

et al. 2016

Vet World

View full text Add to dashboard Cite

Aim:Infectious porcine bronchopneumonia, caused by Pasteurella multocida, is a widespread disease of major economic significance. Thus, the aim of the present study was to diagnose swine Pasteurellosis using gross, histopathological, and immunopathological approaches in the swine population of Punjab and to compare the efficacy of immunohistochemical (IHC) techniques with conventional diagnostic techniques.Materials and Methods:A total of 71 adult swine lung samples showing gross pneumonic changes were collected along with the associated lymph nodes to carry out the study. The collected samples were then processed for histopathological and IHC studies.Results:Out of the total 71 lung samples, 26 samples were found to be suspected for Pasteurellosis as per the microscopic changes observed, and out of these 26 samples, 16 cases were confirmed to be positive for Pasteurellosis by IHC. Varied macroscopic changes noted in lungs were pneumonic patches with consolidation of many lobes, congestion, and focal hemorrhages. Main lesions associated with lymph nodes were its enlargement and hemorrhages. Histologically, the lung showed fibrinous and suppurative bronchopneumonia, multifocal suppuration, thickening of septa with fibrin combined with cellular infiltration and edema. The higher IHC expression of P. multocida was seen in the bronchial epithelium besides in alveolar and bronchial exudate. Moreover, on comparing the histopathological and IHC scores which were calculated on the basis of characteristic microscopic lesions and number of antigen positive cells, respectively, a significant positive correlation (r=0.4234) was found.Conclusion:It was concluded that swine population of Punjab is having P. multocida infection. The gross and histopathological lesions can be helpful in the preliminary diagnosis of Pasteurellosis but needs to be supplemented by other immunodiagnostic tests. Moreover, IHC technique proved to be a specific, reliable, precise, and rapid technique to supplement these conventional methods of diagnosis for Pasteurellosis.

show abstract

“…Some authors agree that some of aforementioned pathogens (such as P. multocida) can be considered the primary respiratory pathogen, because of their ability to cause lesions in the lung tissue on their own (7).…”

Section: Introductionmentioning

confidence: 99%

The Prevalence of Pasteurella Multocida from Farm Pigs in Serbia

Radanović¹,

Žutić²,

Jakic-Dimic³

et al. 2016

Macedonian Veterinary Review

View full text Add to dashboard Cite

The investigations covered a total of 234 lungs from necropsied pigs with different pneumonic lesions, from 6 farrow-to-finish pig farms during 2013 and 2014. The samples were inoculated on selective culture media and aerobically incubated at 37 o C and in carbon dioxide condition. The isolated bacterial colonies were further characterised morphologically and biochemically. The identification was confirmed using the BBL Crystal, E/N, G/P ID Kit (Becton Dickinson). For determination of the type of Pasteurella multocida, the PCR method was used. The findings showed that bacteria were isolated from 202 (86%) out of 234 examined lung samples. The pure isolates of Pasteurella multocida were obtained from 71 (35 %) samples. Out of the remaining 29 (14% ) examined lung samples, 9, 8, 7 and 5 examined lung samples were shown as mixed cultures of Pasteurella multocida and Streptococcus spp., Arcanobacterium pyogenes, Actinobacillus pleuropneumoniae and Haemophilus parasuis, respectively. The PCR method confirmed that all 15 investigated strains of P. multocida belong to type A.

show abstract

Isolation, characterization, antibiogram and pathology of Pasteurella multocida isolated from pigs

Cited by 10 publications

References 8 publications

Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine

Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine

Histopathological and immunohistochemical approaches for the diagnosis of Pasteurellosis in swine population of Punjab

The Prevalence of Pasteurella Multocida from Farm Pigs in Serbia

Contact Info

Product

Resources

About