Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine

George, Shiney; Barman, Nagendra Nath; Nath, Anjan Jyoti; Sarma, B. J.

doi:10.14202/vetworld.2015.438-442

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…The precision of BAL in the diagnosis of pneumonia in swine was reported in this study and correlated with findings of Ganter et al (1993) and Delventhal et al (1992). Bronchoalveolar lavage was used to study experimental respiratory infections with Pasteurella multocida (Müller et al, 2003;George et al, 2015) and Influenza virus (Charley et al, 1980), and also to study induction of lymphocyte migration within the local immune system of the lung (Pabst & Gehrke, 1990;Huang et al, 1990;Pabst et al, 1995;Pabst, 1996). However, experience is needed in the measurement and interpretation of acellular components in BAL fluid in this specie.…”

Section: Discussionsupporting

confidence: 87%

Haematology, bronchoalveolar cellular changes and pathology of swine pneumonia in Nigeria

Emikpe¹,

Jarikre²,

Adediran³

et al. 2018

Sokoto J. Vet. Sc.

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Respiratory diseases are of considerable economic importance in pigs, and less emphasis is on the diagnostic approaches in porcine health in our environment. The aim of this study was to compare the diagnostic accuracy of haematology, gross, bronchoalveolar lavage (BAL) and histopathological changes of pneumonia in pigs. The study was conducted at a municipal abattoir for over three months. A total of 146 finished pigs were clinically examined. Blood samples were taken, while the plucks were examined for lung lesions. Lavage samples and lung sections were taken for BAL and histological examinations. Six breeds were slaughtered comprising Large White 112 (76.7%), Mixed Breed 15 (10.3%), Duroc 7 (4.8%), Local 6 (4.1%), Hampshire 4 (2.7%), and Large Black 2 (1.4%). Based on sex, 71 (48.6%) were males while, 75 (51.4%) were female. Grossly, there was pneumonia in 92 (63.0%) of the pigs and the mean pulmonary consolidation score was 9.6±1.2. The large white breed had the highest consolidation score. Also, the right and left caudal lobes had the highest consolidation scores. Clinically, there was mild anaemia and leukocytosis, and BAL fluid cellular differential showed increased lymphocytic and neutrophilic counts in the pigs with pneumonia (p<0.05). Histologically, 91 (62.2%) of samples were normal, bronchointerstitial pneumonia was 31 (21.4%), bronchopneumonia was 18 (12.4%), granulomatous pneumonia was 1 (0.7%), bronchiolitis was 4 (2.7%), and verminous pneumonia was 1 (0.7%). Bronchointerstitial pneumonia was the most prevalent pattern. Porcine pneumonia is still important in our environment; the roles of different causal agents need to be elucidated for production of vaccines and control in Nigeria and other parts of West Africa.

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Section: Discussionsupporting

confidence: 87%

Haematology, bronchoalveolar cellular changes and pathology of swine pneumonia in Nigeria

Emikpe¹,

Jarikre²,

Adediran³

et al. 2018

Sokoto J. Vet. Sc.

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Comparison of gene expression of Toll-like receptors and cytokines between Piau and Commercial line (Landrace × Large White crossbred) pigs vaccinated against Pasteurella multocida type D

Sousa

Ribeiro

Dantas

et al. 2017

Research in Veterinary Science

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We aimed to compare Toll-like receptors (TLR) and cytokines expression in local Piau breed and a Commercial line (Landrace×Large White crossbred) pigs in response to vaccination against Pasteurella multocida type D. Seronegative gilts for Pasteurella multocida type D and Mycoplasma hyopneumoniae were used, from which peripheral blood mononuclear cells (PBMC) were collected in four time points (T0, T1, T2 and T3; before and after each vaccination dose). For bronchoalveolar lavage fluid cells (BALF), we set groups of vaccinated and unvaccinated animals for both genetic groups. Gene expression was evaluated on PBMC and BALF. In PBMC, when we analyzed time points within breeds, significant differences in expression for TLRs and cytokines, except TGFβ, were observed for Commercial animals. For the Piau pigs, only TGFβ showed differential expression. Comparing the expression among genetic groups, the Commercial pigs showed higher expression for TLRs after first vaccination dose, while for IL2, IL6, IL12 and IL13, higher expression was also observed in T3 and IL8 and IL10, in T1 and T3. Still comparing the breeds, the crossbred animals showed higher expression for TNFα in T1 and T2, while for TGFβ only in T2. For gene expression in BALF, vaccinated Commercial pigs showed higher expression of TLR6, TLR10, IL6, IL8, IL10, TNFα and TGFβ genes than vaccinated Piau pigs. The Commercial line pigs showed higher sensitivity to vaccination, while in local Piau breed lower responsiveness, which may partly explain genetic variability in immune response and will let us better understand the tolerance/susceptibility for pasteurellosis.

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Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine

Cited by 2 publications

References 17 publications

Haematology, bronchoalveolar cellular changes and pathology of swine pneumonia in Nigeria

Haematology, bronchoalveolar cellular changes and pathology of swine pneumonia in Nigeria

Comparison of gene expression of Toll-like receptors and cytokines between Piau and Commercial line (Landrace × Large White crossbred) pigs vaccinated against Pasteurella multocida type D

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