Isolation, Characterization and Identification of Phosphate- and Potassium- Solubilizing Bacteria from Weathered Materials of Granite Rock Mountain, That Son, an Giang Province, Vietnam

Abstract

Abstract:Two hundred and thirty-seven bacterial isolates from 118 sample soils/weathered granite rock of That Son Mountain, An Giang province, Vietnam were isolated on Aleksandrov medium. Their colonies were round or irregular, white to yellow and their shape was rod, motile. Thirty four of two hundred and thirty-seven bacterial isolates were capable of dissolving both phosphate and potassium and seven strains had high phosphate and potassium dissolution capacity (>15 mg l -1 P 2 O 5 and >10 mg l -1 K 2 O). These thirty four strains were identified by using PCR technique with specific primers fd1 and rP and DNA sequencing. The results of DNAsequencing were compared with GenBank database of NCBI by BLAST N software. The sequences from selected phosphate and potassium isolates (34 isolates) showed high degrees of similarity to those of the GenBank references (between 99% and 100%). Among these sequenced isolates, 8 isolates belong to Bacilli (23.53%) and 26 isolates belong to Proteobacteria (76.47%) including 21 alpha-proteobacteria (61.76%) and 5 gamma-proteobacteria (14.71%). Based on Pi value (nucleotide diversity), Proteobacteria group had the highest Theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and AlphaProteobacteria group had the highest values in comparistion with two groups. These results showed that 3 strains including CA09 (Agrobacterium tumefaciens), CA29 (Rhizobium tropici) and K16B (Azotobacter tropicalis) proposed as potential microbial inoculants or biofertilizers for sustainable crop production in sandy acid soil in Vietnam because of their benefits and biosafety.

Effect of Biodive Fertilizer on N, P, and K Nutrition Status and Growth and Result of Two Gogo Rice Varieties in Coastal Area

“…Potassium solubilizing microbes can dissolve potassium from binding insoluble potassium to a medium through secretion organic acids and potassium solubilizing microbes can utilize dissolved potassium in a medium to formation of new cells, resulting in binding (immobilization) potassium by microbes. Several groups of K solubilizing bacteria are known to be able to dissolve K such as Pseudomonas, Burkholderia, Azotobacter, Rhizobium, Bacillus, and Paenibacillus (Singh et al, 2010;Don and Diep, 2014).…”

Perubahan Karakteristik Tanah Dan Penampilan Beberapa Varietas Padi Gogo Pada Kawasan Pesisir Dengan Penambahan Pupuk Hayati Dan Biokompos

“…Mikroba pelarut kalium dapat melarutkan kalium dari ikatan kalium tak larut pada suatu media melalui sekresi asam-asam organik dan mikroba pelarut kalium dapat memanfaatkan kalium terlarut pada suatu media untuk pembentukan sel-sel baru, sehingga terjadi pengikatan (immobilisasi) kalium oleh mikroba (Basak & Biswas, 2009). Beberapa kelompok bakteri pelarut K diketahui mampu melarutkan K seperti Pseudomonas, Burkholderia, Azotobacter, Rhizobium, Bacillus, dan Paenibacillus (Hu et al, 2006;Singh et al, 2010 ;Don & Diep, 2014).…”

Identification of Potential Isolate Phosphate Solubilizing Bacteria Used 16S rRNA Gene

“…In terms of the context, biofertilizer emerges as an essential component for sustaining healthy land because it consists of different types of microbes that live in the rhizosphere. They can promote plant growth by fixing nitrogen from the air, transforming macronutrients into micronutrients, and solubilizing phosphate, zinc, and potassium into available forms (Don & Diep, 2014;Mishra et al, 2013). In addition, the microbe inoculant is used as a biocontrol agent by producing an antagonist compound and interrupting pathogen quorum sensing.…”