The Government of Indonesia has regulated the acceleration of food self-sufficiency for rice, corn, and soybean (Pajale) through Minister of Agriculture Regulation No.14 of 2015. It is highly suggested that before the implementation of this program. It is necessary to identify different foods potential from one region to another. Hence, this research aims to identify Pajale commodities that are seeded, analyze the specialization and localization of Pajale commodities, and identify priority of Pajale commodities that can be developed.
Plants always interact with microorganisms either negatively or positively during their life cycle. The Phosphate Solubilizing Bacteria (PSB) group is one example that has some beneficial effects on the plant. PSB can transform insoluble soil inorganic phosphates into soluble phosphorous so plants can absorb them. This research aimed to test the isolate's ability to solubilize phosphorous and use the 16S rRNA marker gene to identify it. Testing the ability of bacteria to solubilize phosphorous on Pikovskaya's agar medium used a qualitative method by observing the halo zone in the colony around it. Then the bacterial isolate was identified by analyzing the result of sequencing the 16S rRNA gene. The RZ02 bacterial isolate showed the highest performance in solubilizing phosphorous, with a 9.1 mm solubilization index. The gene of 16S rRNA was carried out by using primers 27F and 1492R with an amplicon size of 1500 base pairs. Sequencing analysis and construction of a phylogeny tree were conducted by the Mega X neighbor-joining method and showed 100% similarity with Pseudomonas aeruginosa OIS 481. The solubilization index indicated that the bacteria had a potential candidate to be one of the inoculant components for biofertilizer.
Fusarium wilt disease on banana has been known as panama disease one of the main diseases that cause huge losses for banana farmers. It is caused by the soil-borne fungal pathogen, Fusarium oxysporum f.sp cubense (Foc), which is very hard control because it is saprophytic in the soil. The mold infiltrates the root to vascular tissue that induces yellowing on the leaf, so this pathogen can attack the root, stem dan leaf. The research aimed to search bacteria from the banana rhizosphere that have an antifungal activity to inhibit Foc growth. Bacteria was isolated by serial dilution then was spread on King’s B agar medium incubation 28oC (room temperature). Four quadrants in vitro test on PDA medium used twenty bacterial from isolation, from the test was obtained six isolates have the potential to inhibit the growth of Foc. Based on percentage inhibition radial growth four isolates that have inhibition 50% over which TR2 was the highest at 79.07%. The in vitro test confirmed that bacteria from the banana rhizosphere have potential as biocontrol agent because it was able to inhibit the Foc growth.
This research aimed to determine the physicochemical characteristics of durian seed flour and to develop the utilization of durian seeds. The analysis was performed using a factorial complete randomized design consisting of two factors, namely the concentration of sodium metabisulphite (N) and the salt concentration (G). The concentration of sodium metabisulphite (N) gave a very significant effect on moisture content, ash content, and the value of L; and no significant effect on fat content and protein content. Furthermore, salt concentration (G) had a very significant effect on ash content, fat content and the value of L; a significant effect on the moisture content and protein content. The interaction between the two factors had a highly significant effect on ash content and the value of L; and no significant effect on moisture content, fat content and protein content. Durian seed flour with a concentration of 3000 ppm sodium metabisulphite and a salt concentration of 4% yield produced by best durian seed flour.
Plant biomass from waste agricultural can be transformed to useful like bioethanol and organic fertilizer in industry and agriculture using bacterial cellulase is one alternative to convert cellulose become organic fertilizer. This research had an objective to obtain potential bacterial cellulase isolated from the soil around of cowshed in Institut Pertanian Bogor. Bacteria were isolated from soil had used serial dilution that spread in 1% Carboxy Methyl Cellulose Medium (CMC 1%) and it's incubated at 37oC for 48 hours. Qualitative test for bacteria producing cellulose was carried out by picking the selected colony in the centered of CMC medium with adding congo red 1%. Based on the results were obtained three potential isolates could produce cellulose that were KS 0.1, KS 0.7 and KS 9.1. The highest cellulolytic index is 1.33 that produced by KS 0.1. The test quantitative activity of KS 0.1 using spectrophotometry method showed that the highest of enzyme activity at the first day and lowest eighth day during incubation period.
Key Words: bacteria, cellulose, soil around of cowshed
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