Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance

Rajasekar, Periyannan; Palanisamy, Subramanian; Anjali, Ravichandran; Vinosha, Manoharan; Elakkiya, Meyyanathan; Marudhupandi, Thangapandi; Tabarsa, Mehdi; You, SangGuan; Prabhu, Nagesh

doi:10.1016/j.ijbiomac.2019.09.024

Cited by 84 publications

(36 citation statements)

References 62 publications

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“…Chen et al showed that the monosaccharides of PAP were mainly composed of rhamnose, fucose, arabinose, xylose, mannose, and glucose with the molar ratio of 3.42:0.76:0.34:0.53:0.43:0.59 [ 5 ]. The results of Pr et al showed that PAP contained a relatively large proportion of galactose [ 9 ]. Ma et al found that PAP was mainly composed of rhamnose, glucose, and galactose [ 6 ].…”

Section: Resultsmentioning

confidence: 99%

“…At present, researchers on PAP have made progress in the separation, purification, and determination of chemical structure and biological activity, but there are still many problems to be solved. Most studies have been based on crude polysaccharides, but crude polysaccharides may contain multiple mixed sugar components and small molecular impurities, so it is difficult to obtain a definitive conclusion from these studies [ 6 , 7 , 8 ] In addition, there have been some studies on the structure of isolated and purified PAP by FT-IR, NMR, and GC-MS, but research on the structure has not been thorough enough [ 5 , 9 , 10 ]. The detailed structure of polysaccharides was the foundation for studying their potential mechanisms and structure-activity relationships.…”

Section: Introductionmentioning

confidence: 99%

“…With the continuous development of sequencing technology, RNA-Seq has become an effective tool for excavating and identifying new transcripts. The above studies indicate that PAP has antiviral and antioxidant effects [ 5 , 8 , 9 ], and PRV infection RAW264.7 cells may cause the differential expression of circRNA, miRNA, and mRNA [ 29 , 30 , 31 ]. In this study, the non-coding RNA sequencing data of PRV-infected RAW264.7 cells and PAP-1 acting on PRV-infected RAW264.7 cells from the GEO database were analyzed, and the circRNA-miRNA-mRNA network was successfully constructed, which has the characteristics of highlighting specific molecular functions and mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from Arthrospira platensis

et al. 2021

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To investigate the structure of Arthrospira platensis polysaccharide (PAP) (intracellular polysaccharide) and the antioxidant activity of the first component of PAP (PAP-1) on pseudorabies virus (PRV) -infected RAW264.7 cells. The PAP was separated and purified by the Cellulose DE-52 chromatography column and Sephacryl S-200 high-resolution gel column to obtain PAP-1. The antioxidant activity and regulation of PAP-1 on PRV-infected RAW264.7 cells of circRNA-miRNA-mRNA network were investigated by chemical kit, Q-PCR, and ce-RNA seq. The results indicated that the molecular weight (Mw) of PAP-1, which was mainly composed of glucose and eight other monosaccharides, was 1.48 × 106 Da. The main glycosidic bond structure of PAP-1 was →4)-α-D-Glcp-(1→. PAP-1 may be increased the antioxidant capacity by regulating the circRNA-miRNA-mRNA network in PRV-infected RAW264.7 cells. This study provided a scientific foundation for further exploring the antioxidant activity of PAP-1 based on its structure.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from Arthrospira platensis

et al. 2021

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“…[Jayaram, et al, 2015;Ahmed, et al, 2015]. Various investigators optimised the extraction conditions and partially characterized the sulphated Sp [Rajasekar, et al, 2019] and explored for the potential antioxidant properties in in vitro models [Kurd, et al, 2015]. It has been shown that sulphated spirulina polysaccharides exhibited the greater antibacterial activity and improved the reproductive performance in zebra fish [Rajasekar, et al, 2019].…”

Section: Discussionmentioning

confidence: 99%

Water soluble polysaccharides from Spirulina platensis exhibited cyto/DNA protection and suppress the growth of gastric cancer cells via modulation of galectin-3

Uppin

Dharmesh

Ravi

2021

Preprint

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Polysaccharides from natural sources play a significant role in the management of different cancer types including gastric cancer. In this study, we reported the effect of spirulina polysaccharide (Sp) on galectin-3 modulatory activity on gastric cancer cells. The polysaccharide was isolated from the spirulina biomass, characterized, and the in silico, in vitro studies are carried out to assess the bioactivities. The isolated Sp possessed average molecular weight of 1457 kDa, and galactose (42%) as major sugar along with Rhamnose, Arabinose, Xylose, and Mannose. Further, characterization of Sp by FT-IR and NMR spectrum indicated the presence of (β1-4D) galactose sugar with galactoarabinorhamnoglycan backbone. Among the monosaccharides, galactose showed highest binding affinity with galectin-3 protein as evidenced by the in silico interaction study. The obtained Sp, inhibited the proliferation of AGS gastric cancer cells by 48 % without affecting normal NIH/3T3 cells as opposed to doxorubicin, a known anticancer drug. Also, Sp exhibited galectin-3 mediated haemagglutination inhibition with MIC of 9.37 μg/mL compared to galactose 6.25 μg/mL, sugar specific to galectin-3. The Sp treatment significantly (p<0.05) lowered the expression of galectin-3 by 32 % compared to untreated control cells. In addition, Sp exhibited the potent cytoprotection in RBCs, Buccal cells, and DNA exposed to oxidants. Thus, the findings suggest that the polysaccharide from spirulina offer a promising therapeutic strategy in the management of gastric cancer in addition to its currently known nutritional and pharmaceutical applications.

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“…Polysaccharides extracted from of S. platensis are water-soluble; it has been proven that they have antitumor, antioxidation, antiaging, and antivirus properties [58]. Rajasekar et al investigated the structural characterization and bioactive potential of sulfated polysaccharides from S. platensis and isolated a fraction containing glucose, rhamnose, xylose, fucose, mannose, and galactose as main sugars with Mw of 1016 kDa [59].…”

Section: Bacterial Polysaccharidesmentioning

confidence: 99%

Selenium-Containing Polysaccharides—Structural Diversity, Biosynthesis, Chemical Modifications and Biological Activity

2021

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Selenosugars are a group of sugar derivatives of great structural diversity (e.g., molar masses, selenium oxidation state, and selenium binding), obtained as a result of biosynthesis, chemical modification of natural compounds, or chemical synthesis. Seleno-monosaccharides and disaccharides are known to be non-toxic products of the natural metabolism of selenium compounds in mammals. In the case of the selenium-containing polysaccharides of natural origin, their formation is also postulated as a form of detoxification of excess selenium in microorganisms, mushroom, and plants. The valency of selenium in selenium-containing polysaccharides can be: 0 (encapsulated nano-selenium), IV (selenites of polysaccharides), or II (selenoglycosides or selenium built into the sugar ring to replace oxygen). The great interest in Se-polysaccharides results from the expected synergy between selenium and polysaccharides. Several plant- and mushroom-derived polysaccharides are potent macromolecules with antitumor, immunomodulatory, antioxidant, and other biological properties. Selenium, a trace element of fundamental importance to human health, has been shown to possess several analogous functions. The mechanism by which selenium exerts anticancer and immunomodulatory activity differs from that of polysaccharide fractions, but a similar pharmacological effect suggests a possible synergy of these two agents. Various functions of Se-polysaccharides have been explored, including antitumor, immune-enhancement, antioxidant, antidiabetic, anti-inflammatory, hepatoprotective, and neuroprotective activities. Due to being non-toxic or much less toxic than inorganic selenium compounds, Se-polysaccharides are potential dietary supplements that could be used, e.g., in chemoprevention.

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Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance

Cited by 84 publications

References 62 publications

Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from Arthrospira platensis

Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from Arthrospira platensis

Water soluble polysaccharides from Spirulina platensis exhibited cyto/DNA protection and suppress the growth of gastric cancer cells via modulation of galectin-3

Selenium-Containing Polysaccharides—Structural Diversity, Biosynthesis, Chemical Modifications and Biological Activity

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