Isolation and Selection of Fungi for Degrading Saturated Hydrocarbons, Aromatic Hydrocarbons and NSO Compounds

Abstract

Petroleum hydrocarbons are recalcitrant contaminants that have various impacts on the natural ecosystem. Microorganisms have been widely used in recent years to remove the hydrocarbons that are present in crude oil. The objective of this research was to isolate and select microorganisms that have the potential to degrade hydrocarbons present in mangrove sediments. In present study, we have isolated fungi from clean sediment contaminated with different fractions (i.e., saturated hydrocarbons, aromatic, and no hydrocarbon compounds (NSO)) of two types of oil. There were two types of oxidation tests were performed: one in multi well plates and the other in stirring and temperature control. Screening tests were conducted to detect the biodegradation of petroleum fractions with 72 fungal isolates over a period between 12 and 48 hours. With the two oxidation tests, the 2,6-dichlorophenol-indo phenol (DCPIP) indicator made it possible to select fungi with the potential to degrade the three main fractions of oil in the Reconcavo and Campos Basins. It was observed that the fungal isolates in the cleaned sediment were able to oxidize the three fractions of both types of oil. It was also concluded that some isolated strains oxidize oil faster and more efficiently than others. The formation of a consortium with the isolated consortium was a potential for the increase of the degradation of oil in the environment.

Degradation Characteristics of Oil Degrading Candida tropicalis

“…A strain of G-94 with petroleum as the sole carbon source was isolated from Oil degradation is limited by many factors in the soil [14] [15]. The development and utilization of the genetic resources of G-94 for petroleum degradation and their application in the remediation of petroleum contaminated soils should be further studied.…”

Bioprospection of ligninolytic enzymes from marine origin filamentous fungi

“…(N89). These microorganisms were selected for their ability to tolerate / degrade petroleum hydrocarbons (Lima et al 2017(Lima et al , 2018 and therefore constitute a mixed microbial consortium protected by the patent with deposit number BR 10 2021 002341 4 (Lima et al 2021), at the Instituto Nacional de Propriedade Industrial (INPI) and endorsed at the National Biotechnology Information Center (NCBI) under access numbers MW855899, MW855902, MW855901, MW855900, MW855903, respectively. They were grown on Sabouraud Dextrose Agar (SDA) (Kasvi®, Brazil) and maintained at 4 ° C for further analysis.…”

Biotechnology Potential of Marine Fungi Degrading Plant and Algae Polymeric Substrates

“…The redox indicator DCPIP (redox potential +0.217 V) is used for the rapid and simple colorimetric determination of the different types of oil biodegradation profiles for the hydrocarbon-degrading microorganisms based on the decoloration of reduced molecules of the substrate ( Bidoia et al, 2010 ). The oxidation tests with the use of DCPIP allowed selecting the fungi degrading three main fractions of oil in the Reconcavo and Campos Basins: saturated hydrocarbons, aromatic, and non-hydrocarbon compounds ( Lima et al, 2017 ). The isolates of A. niger , Penicillium documbens and Cochliobolus lunatus collected from Pensacola beach (Gulf of Mexico) had the ability to degrade crude oil in the presence of redox indicator, decreasing the hydrocarbon weight approximately by up to 10 % during 7 days ( Al-Nasrawi, 2012 ).…”