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The compartment niche is the main reason behind the shifts in endophytic bacterial communities. Long‐term organic greenhouse exerted limited influence on the variations of endophytic bacterial communities. Organic greenhouse and root had more complex co‐occurrence networks than conventional greenhouse and stem, respectively. Cultivable method results found that Protecbacteria, Bacteriodes, and Actinobacteria are the dominant phyla in the endophytes.

Objective: The aim of study is to obtain a strain which can effectively degrade oil. Methods: By enrichment, domestication and separation of culture from the sample which comes from Qianjiang Guanghua Oilfield, the pure culture (G-40) was selected from the medium supplemented with oil as the sole source of carbon. The G-40 was preliminarily identified and classified by morphological observation, physiological and biochemical determination and sequence analyses of 16S rDNA. Effects of temperature, pH, oil concentration, salt concentration, inoculation amount, N and P source on the degradation of the oil were studied by single factor test. Meanwhile, the effects of temperature, pH, oil concentration and salt concentration were optimized by orthogonal design. Result: G-40 was isolated and identified as Brevibacillus laterosporus. The orthogonal design showed that the degradation rate of oil could reach 20.81%, 24.34% and 31.15% at 10 d, 20 d and 40 d under the optimal conditions which was 35˚C, initial pH 7.3, oil concentration 0.6%, salt concentration 0.5%, inoculum amount 8%, the optimal N and P was (NH 4 ) 2 SO 4 and K 2 HPO 4 . Conclusion: This study provides microbial resources for bioremediation of petroleum contaminated soil.

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